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A new medium is described for the selection and identification of Pseudomonas aeruginosa. It contains glycerol, mannitol, acetamide, cetrimide, a trace of peptone and phenol red. On this medium incubated at 42°C, nearly all cultures of Ps. aeruginosa, but only very few other bacteria, form colonies or areas of growth surrounded by a red zone. The medium may be used either for the direct enumeration of Ps. aeruginosa or to establish the presence of this organism in liquid enrichment cultures.
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