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A comparison of Hoyle’s medium and Billings’ modification of Tinsdale’s medium was made. Hoyle’s medium was significantly better for the primary isolation of Corynebacterium diphtheriae from swabs. The only advantage of Tinsdale’s medium was that it could be used by untrained people, whereas the use of Hoyle’s medium required experience. The formation of a halo on Tinsdale’s medium is recommended as a specific confirmatory test for the identification of C. diphtheriae and C. ulcerans.
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