1887

Abstract

SUMMARY

A chloroform-soluble, extracellular product of was obtained from sloppy-agar cultures. Pyrogallol 1-monomethyl ether, 1-methoxyphenazine, 1-hydroxyphenazine and pyocyanin were chemically synthesised and also tested for an inhibitory effect on mitochondrial respiration. It was found that the substance and 1-hydroxyphenazine inhibited the uptake of oxygen by mouse liver mitochondria utilising sodium succinate.

In a infection this substance would be released into the bloodstream, accumulate in the liver and cause cytolysis, thus contributing to the complex mechanism of pathogenicity.

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1971-05-01
2024-04-25
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References

  1. Asay L. D., Koch R. 1960; Pseudomonas infections in infants and children. New Engl. J. Med 262:1062
    [Google Scholar]
  2. Bouchard C. 1889; Influence qu’exerce sur la maladie charbonneuse 1’inoculation du bacille pyocyanique. C. r. hebd. Seanc. Acad. Sci., Paris 108:713
    [Google Scholar]
  3. Budzikiewicz H., Djerassi C., Williams D. H. 1967; Mass spectrometry of organic compounds. London p 332
    [Google Scholar]
  4. Colebrook L., Francis A. E. 1941; Elimination of streptococci from superficial wounds by sulphanilamide powder. Lancet 1:271
    [Google Scholar]
  5. Corbett J. F. 1964; The infrared and ultraviolet spectra of hydroxyphenazines. Spectro-chim. Acta 20:1665
    [Google Scholar]
  6. Hendry E. B. 1948; The preparation of iso-osmotic phosphate buffer solutions. Edinb. Med. J 55:142
    [Google Scholar]
  7. Hettche O. H. 1933; Untersuchungen ủber die Natur der bactericiden und hamolytischen Bestandteile der Pyocyaneuslipoide. I Mitteilung. Klin. Wschr 12:1804
    [Google Scholar]
  8. Hewitt L. F. 1950; Oxidation-reduction potentials in bacteriology and biochemistry. 6th ed Edinburgh:
    [Google Scholar]
  9. Hoffman M. A., Finberg L. 1955; Pseudomonas infections in infants associated with high humidity environments. J. Pediat 46:626
    [Google Scholar]
  10. Holliman F. G., Johnstone R. A. W., Millard B. J. 1967; Aspects of mass spectra of organic compounds. Part V Some phenazines. J. Chem. Soc (C) 2351
    [Google Scholar]
  11. Jackson D. M., Lowbury E. J. L., Topley Elizabeth. 1951; Pseudomonaspyocyanea in burns. Its role as a pathogen, and the value of local polymyxin therapy. Lancet 2:137
    [Google Scholar]
  12. Jones R. J., Jackson D. M., Lowbury E. J. L. 1966; Antiserum and antibiotic in the prophylaxis of burns against Pseudomonas aeruginosa . Br. J. Plast. Surg 19:43
    [Google Scholar]
  13. King Elizabeth O., Ward Martha K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. J. Lab. Clin. Med 44:301
    [Google Scholar]
  14. Kramer H. 1935; Neue Untersuchungen ủber die antagonistische Wirkung des Bacillus pyocyaneus . Z. ImmunForsch. exp. Ther 84:505
    [Google Scholar]
  15. KuCera M., Lysenko O. 1968; The mechanism of pathogenicity of Pseudomonas aeruginosa. V. Isolation and properties of the proteinases toxic for larvae of the greater wax moth Galleria mellonella L . Folia microbiol 13:288
    [Google Scholar]
  16. Lilley A. B., Bearup A. J. 1928; Generalized infections due to Pseudomonas aerugin-osa (Bacillus pyocyaneus) with study of characteristics of local strains of organism. Med. J. Austral 1:362
    [Google Scholar]
  17. Liu P. V. 1957; Survey of hemolysin production among species of pseudomonads. J. Bad 74:718
    [Google Scholar]
  18. Liu P. V. 1966a; The roles of various fractions of Pseudomonas aeruginosa in its patho-genesis. II. Effects of lecithinase and protease. J. Infect. Dis 116:112
    [Google Scholar]
  19. Liu P. V. 1966b; The roles of various fractions of Pseudomonas aeruginosa in its patho-genesis. III. Identity of the lethal toxins produced in vitro and in vivo . J. Infect. Dis 116:481
    [Google Scholar]
  20. Liu P. V., Abe Y., Bates Janice L. 1961; The roles of various fractions of Pseudomonas aeruginosa in its pathogenesis. J. Infect. Dis 108:218
    [Google Scholar]
  21. Lowbury E. J. L. 1954; Air-conditioning with filtered air for dressing burns. Lancet 1:292
    [Google Scholar]
  22. Lowbury E. J. L., Fox Jean. 1954; The epidemiology of infection with Pseudomonas pyocyanea in a burns unit. J. Hyg., Camb 52:403
    [Google Scholar]
  23. Morita Y. 1966; Studies on phenazines XXIX. Mass spectrometric and chromatographic studies. Chem. Pharm. Bull., Tokyo 14:426
    [Google Scholar]
  24. Nelson E. L., Berk R. S. 1960; Irradiation, immunization and sensitivity to toxic substances: considerations in a concept of cellular immunity. Ann. N,. Y. Acad. Sci 88:1246
    [Google Scholar]
  25. Neter E., Weintraub D. H. 1955; An epidemiological study on Pseudomonas aeruginosa (Bacillus pyocyaneus) in premature infants in the presence and absence of infection. J. Pediat 46:280
    [Google Scholar]
  26. Pons R. 1927; Existence de la melioidose en Cochinchine. Etude de l’agent etiologique: Bacillus pseudo-mallei (Whitmore, 1913); Bacillus Whitmori (Stanton et Fletcher, 1923). Annls Inst. Pasteur, Paris 41:1338
    [Google Scholar]
  27. Rabin E. R., Graber C. D., Vogel E. H. Jr, Finkelstein R. A., Tumbusch W. A. 1961; Fatal Pseudomonas infection in burned patients. A clinical, bacteriologic and anatomic study. New Engl. J. Med 265:1225
    [Google Scholar]
  28. Rogers K. B. 1960; Pseudomonas infections in a children’s hospital. J. Apph Bad 23:533
    [Google Scholar]
  29. Rugstad H. E. 1966; Kininase production by some microbes. Br. J. Pharmac. Chemother 28:315
    [Google Scholar]
  30. Rugstad H. E. 1967a; Purification of a kinin-inactivating enzyme from cultures of Pseudomonas aeruginosa . Br. J. Pharmac. Chemother 30:134
    [Google Scholar]
  31. Rugstad H. E. 1967b; Characterization of a plasma kinin-inactivating enzyme produced by Pseudomonas aeruginosa . Br. J. Pharmac. Chemother 30:425
    [Google Scholar]
  32. Salvin S. B., Lewis M. L. 1946; External otitis with additional studies on the genus Pseudomonas . J. Bad 51:495
    [Google Scholar]
  33. Schaffer A. J., Oppenheimer E. H. 1948; Pseudomonas (pyocyaneus) infection of gastro-intestinal tract in infants and children. Sth. Med. J., Bham, Ala 41:460
    [Google Scholar]
  34. Schneider W. C. 1948; Intracellular distribution of enzymes. Ill The oxidation of octanoic acid by rat liver fractions. J. Biol. Chem 176:259
    [Google Scholar]
  35. Schoental Regina. 1941; The nature of the antibacterial agents present in Pseudomonas pyocyanea cultures. Br. J. Exp. Path 22:137
    [Google Scholar]
  36. Stewart-tull D. E. S., White R. G. 1967; The influence of age of culture on the production of adjuvant-active peptido-glycolipids by saprophytic mycobacteria. Immunology 12:349
    [Google Scholar]
  37. Surrey A. R. 1946a; Pyocyanine. Org. Synth 26:86
    [Google Scholar]
  38. Surrey A. R. 1946b; Pyrogallol 1-monomethylether. Org. Synth 26:90
    [Google Scholar]
  39. Wrede F., Strack E. 1924; Über das Pyocyanin, den blauen Farbstoff des Bacillus pyocyaneus I . Z. physiol. Chem 140:1
    [Google Scholar]
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