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Several extracts prepared by the action of surface active agents on live cells of the rough Br. abortus strain 45/20 were found to protect mice and guinea-pigs against challenge with virulent Br. abortus. Incorporation in oily emulsions made little difference to the degree of immunity elicited.
An extract prepared by treatment of living cells of strain 45/20 with sodium dodecy1 sulphate followed by ethanol precipitation induced substantial immunity in cattle, despite the fact that no adjuvant was used. The extract was devoid of the smooth O somatic antigen and probably free from lipopoly-saccharide. The high protein and carbohydrate content suggested that the immunogen could be a glycoprotein.
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