1887

Abstract

Summary

The survival of two colicine-sensitive strains of when grown in mixed culture with each of three colicine-producing strains was investigated. The universal colicine indicator strain, B/TKM, was completely eliminated during mixed culture with each of the colicinogenic strains, whereas colicine-resistant mutants of it showed normal growth. The second colicine-sensitive strain, no. C600/21, was also eliminated by the colicine-B-producing strain, but not by either the colicine-E- or E-producing strains. The bacteria that were refractory to colicine E or E remained viable even in high concentrations of the colicine yet were unable to multiply; they regained full colicine sensitivity after growth in peptone water or when subcultured with a non-colicine producing strain of , but not when grown with a strain producing the same colicine. The refractory cells were non-colicinogenic and free colicine was present in the supernatant fluids of the mixed cultures. After treatment with trypsin to destroy the absorbed colicine the refractory cells regained their colicine sensitivity.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-4-1-63
1971-02-01
2019-12-08
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-4-1-63
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error