Several strains of TRIC agent produced agglutinins for mouse and rat erythrocytes during growth in embryonated hens’ eggs. The agglutinins appeared irregularly and in a low percentage of inoculated eggs. A small inoculum dose was more likely to give a good yield, and a lethal inoculum failed to produce detectable amounts of haemagglutinin. The haemagglutinins were separable from the infectious particles and, with the exception of those associated with the T’ang strain, were unstable on storage. The agglutinin for rat erythrocytes was more often present in the allantoic fluid whilst the mouse cell agglutinin was more cell-associated. The T’ang strain also produced a haemolysin.
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