1887

Abstract

SUMMARY

Sucrose density-gradient centrifugation was used for the study of serum IgM and IgG antibodies in rubella infection in early pregnancy. Seventeen women with proven rubella in the first trimester of pregnancy were tested and all were found to have an IgM antibody response to the virus. IgM antibody was present for 1 mth following the onset of illness but not later, and it was absent in women who did not have acute rubella. With one exception, IgM antibodies were not found in women who had recently given birth to an infant with congenital rubella.

The technique involved the collection of six fractions from the density gradient. The second fraction, which contained IgM but was free from IgG, was tested for rubella antibody by means of an HI test. Examination of this one fraction from a single serum collected within 1 mth of the onset of illness was sufficient to confirm or exclude a diagnosis of rubella.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-4-1-107
1971-02-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/4/1/medmicro-4-1-107.html?itemId=/content/journal/jmm/10.1099/00222615-4-1-107&mimeType=html&fmt=ahah

References

  1. Banatvala J. E., Best Jennifer M., Kennedy Elizabeth A., Smith Elizabeth E., Spence Marjorie E. 1967; A serological method for demonstrating recent infection by rubella virus. Br. Med. J 3:285
    [Google Scholar]
  2. Baublis J. V., Brown G. C. 1968; Specific response of the immunoglobulins to rubella infection. Proc. Soc. Exp. Biol. Med 128:206
    [Google Scholar]
  3. Best Jennifer M., Banatvala J. E. 1969; Serological tests for rubella. Lancet 2:546
    [Google Scholar]
  4. Best Jennifer M., Banatvala J. E., Watson D. 1969; Serum IgM and IgG responses in postnatally acquired rubella. Lancet 2:65
    [Google Scholar]
  5. Cooper L. Z., Matters Barbara, Rosenblum Jean K., Krugman S. 1969; Experience with a modified rubella hemagglutination inhibition antibody test. J. Amer. Med. Assoc 207:89
    [Google Scholar]
  6. Fudenberg H. H., Kunkel H. G. 1957; Physical properties of the red cell agglutinins in acquired hemolytic anemia. J. Exp. Med 106:689
    [Google Scholar]
  7. Lehrich J. R., Kasel J. A., Rossen R. D. 1966; Immunoglobulin classes of neutralizing antibody formed after human inoculation with soluble adenoviral antigens. J. lmmun 97:654
    [Google Scholar]
  8. Macrae A. D. 1969; Serological tests for rubella. Lancet 2:220
    [Google Scholar]
  9. Newman S., Horta-Barbosa L., Sever J. L. 1969; Serological tests for rubella. Lancet 2:432
    [Google Scholar]
  10. Rawls W. E. 1970; Rubella virus. In Manual of clinical microbiology ed. by Blair J. E., Lennette E. H., Truant J. P. Bethesda, Md. p 528
    [Google Scholar]
  11. Rawls W. E., Desmyter J., Melnick J. L. 1967; Rubella virus neutralization by plaque reduction. Proc. Soc. Exp. Biol. Med 124:167
    [Google Scholar]
  12. Schluederberg A. 1965; Immune globulins in human viral infections. Nature, Lond 205:1232
    [Google Scholar]
  13. Sever J., White L. R. 1968; Intrauterine viral infections. A. Rev. Med 19:471
    [Google Scholar]
  14. Stewart G. L., Parkman P. D., Hopps Hope E., Douglas R. D., Hamilton Judith P., Meyer H. M. Jr 1967; Rubella-virus hemagglutination-inhibition test. New Engl. J. Med 276:554
    [Google Scholar]
  15. Svehag S. E., Mandel B. 1964; The formation and properties of poliovirusneutralizing antibody. II. 19S and 7S antibody formation: differences in antigen dose requirement for sustained synthesis, anamnesis, and sensitivity to X-irradiation. J. Exp. Med 119:21
    [Google Scholar]
  16. Vesikari T., Vaheri A. 1968; Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. Br. Med. J 1:221
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-4-1-107
Loading
/content/journal/jmm/10.1099/00222615-4-1-107
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error