%0 Journal Article %A García, F. %A Quiros, Eugenia %A Bernal, Maria C. %A de Luis, Belen %A Leyva, Ana %A Piedrola, G. %A Maroto, Maria C. %T Serum hepatitis B virus DNA detection with S- and C-region-directed probes %D 1993 %J Journal of Medical Microbiology, %V 39 %N 6 %P 473-475 %@ 1473-5644 %R https://doi.org/10.1099/00222615-39-6-473 %I Microbiology Society, %X Summary Developments in molecular biology have offered a wide range of nucleic acid probes to detect the genome of hepatitis B virus (HBV). We have tested the ability of two enzyme-linked (alkaline phosphatase) probes to detect HBV-DNA. These hybridise with the S and C regions of the genome of HBV and are used to determine the clinical significance of detecting the two regions. A total of 66 serum samples from patients at different stages of HBV infection was examined. HBV-DNA was detected with at least one of the probes in 17 (85%) patients with HBeAg-positive chronic hepatitis, five (26.3%) with anti-HBe-positive chronic hepatitis and six (66.6%) with acute hepatitis. Although both probes were able to detect as little as 10 pg/ml (2.86 × 106 g. E./ml) of a full length HBV-DNA standard, the C-region-directed probe did not react in one patient with acute hepatitis, two with HBeAg-positive and three with anti-HBe-positive chronic hepatitis. When C-region-directed probes are used for diagnostic purposes, results should always be accompanied by hybridisation with probes directed against other regions showing less variability (e.g. S region). %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-39-6-473