Role of IgG subclass response to outer-membrane proteins in inhibiting adhesion of to epithelial cells Free

Abstract

Summary

The IgG subclass response to the major outer-membrane proteins (OMPs) of was investigated in 11 cystic fibrosis (CF) patients and 10 healthy controls. Inhibition of adhesion of to buccal epithelial cells by the IgG serum fractions from the CF patients has been established previously. The CF patients demonstrated marked heterogeneity in their individual IgG subclass response to pseudomonal OMPs. The predominant IgG1 and IgG4 responses were directed towards OMPs F, H2 and, with IgG1 only, to protein I. Proteins of 42 and 46 kDa primarily elicited an IgG2 response but some patients produced IgG4 antibodies. The IgG3 response varied from very weak in some patients to a strong reaction with proteins D2, E, G and I in others. The range of antigen-specific IgG subclass responses was similar in CF patients whose IgG fractions strongly inhibited the adherence of to epithelial cells and in those whose fractions gave only weak inhibition of adherence. There was no indication that an antibody response towards any particular OMP was implicated in the inhibition of bacterial adherence. Thus, the IgG subclass response to OMPs did not exert a significant effect on adherence when investigated in isolation, but may possibly play some role in combination with other processes.

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1993-12-01
2024-03-28
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References

  1. Yoshihara E, Nakae T. Identification of porins in the outer membrane of Pseudomonas aeruginosa that form small diffusion pores. J Biol Chem 1989; 264:6297–6301
    [Google Scholar]
  2. Gottoh N, Wakabe H, Yoshihara E, Nakae T, Nishino T. Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa outer membrane. J Bacteriol 1989; 171:983–990
    [Google Scholar]
  3. Woodruff WA, Hancock REW. Pseudomonas aeruginosa outer membrane protein F: structural role and relationship to the Escherichia coli OmpA protein. J Bacteriol 1989; 171:3304–3309
    [Google Scholar]
  4. Sokol PA, Woods DE. Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa. Infect Immunol 1983; 40:665–669
    [Google Scholar]
  5. Mutharia LM, Nicas TI, Hancock REW. Outer membrane proteins of Pseudomonas aeruginosa serotype strains. J Infect Dis 1982; 146:770–779
    [Google Scholar]
  6. Mutharia LM, Hancock REW. Characterization of two surface-localized antigenic sites on porin protein F of Pseudomonas aeruginosa. Can J Microbiol 1985; 31:381–386
    [Google Scholar]
  7. Hancock REW, Chan L. Outer membranes of environmental isolates of Pseudomonas aeruginosa. J Clin Microbiol 1986; 26:2423–2424
    [Google Scholar]
  8. Hancock REW, Mouat ECA, Speert DP. Quantification and identification of antibodies to outer-membrane proteins of Pseudomonas aeruginosa in sera of patients with cystic fibrosis. J Infect Dis 1984; 149:220–226
    [Google Scholar]
  9. Shand GH, Pedersen SS, Brown MRW, Hoiby N. Serum antibodies to Pseudomonas aeruginosa outer-membrane proteins and iron-regulated membrane proteins at different stages of chronic cystic fibrosis lung infection. J Med Microbiol 1991; 34:203–212
    [Google Scholar]
  10. Hedstrom RC, Pavlovskis OR, Galloway DR. Antibody response of infected mice to outer membrane proteins of Pseudomonas aeruginosa. Infect Immun 1984; 43:49–53
    [Google Scholar]
  11. Gilleland HE, Gilleland LB, Matthews-Greer JM. Outer membrane protein F preparation of Pseudomonas aeruginosa as a vaccine against chronic pulmonary infection with heterologous immunotype strains in a rat model. Infect Immunol 1988; 56:1017–1022
    [Google Scholar]
  12. Finke M, Duchene M, Eckhardt A, Domdey H, von Specht B-U. Protection against experimental Pseudomonas aeruginosa infection by recombinant P. aeruginosa lipoprotein I expressed in Escherichia coli. Infect Immun 1990; 58:2241–2244
    [Google Scholar]
  13. Hancock REW, Wieczorek AA, Mutharia LM, Poole K. Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: isolation and characterization. Infect Immun 1982; 37:166–171
    [Google Scholar]
  14. Mutharia LM, Hancock REW. Surface localization of Pseudomonas aeruginosa outer membrane porin protein F by using monoclonal antibodies. Infect Immun 1983; 42:1027–1033
    [Google Scholar]
  15. Battershill JL, Speert DP, Hancock REW. Use of monoclonal antibodies to protein F of Pseudomonas aeruginosa as opsonins for phagocytosis by macrophages. Infect Immunol 1987; 55:2531–2533
    [Google Scholar]
  16. Mutharia LM, Hancock REW. Monoclonal antibody for an outer membrane lipoprotein of the Pseudomonas fluorescens group of the family pseudomonadaceae. Int J Syst Bacteriol 1985; 35:530–532
    [Google Scholar]
  17. Sherman PM, Soni R. Adherence of vero cytotoxin-producing Escherichia coli of sterotype 0157:H7 to human epithelial cells in tissue culture: role of outer membranes as bacterial adhesins. J Med Microbiol 1988; 26:11–17
    [Google Scholar]
  18. Sherman P, Cockerill F, Soni R, Brunton J. Outer membranes are competitive inhibitors of Escherichia coli 0157:H7 adherence to epithelial cells. Infect Immun 1991; 59:890–899
    [Google Scholar]
  19. Morrin M, Reen DJ. Inhibition of the adherence of Pseudomonas aeruginosa to epithelial cells by IgG subclass antibodies. J Med Microbiol 1993; 39:459–466
    [Google Scholar]
  20. Sexton M, Reen DJ. Characterization of antibody-mediated inhibition of Pseudomonas aeruginosa adhesion to epithelial cells. Infect Immun 1992; 60:3332–3338
    [Google Scholar]
  21. Hancock REW, Nikaido H. Outer membranes of gram-negative bacteria XIX. Isolation from Pseudomonas aeruginosa PAOl and use in reconstruction and definition of the permeability barrier. J Bacteriol 1978; 136:381–390
    [Google Scholar]
  22. Laemmli UK. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  23. Oakley BR, Kirsch DR, Morris NR. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 1980; 105:361–363
    [Google Scholar]
  24. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–5354
    [Google Scholar]
  25. Shand GH, Pedersen SS, Tilling R, Brown MRW, Hoiby N. Use of immunoblot detection of serum antibodies in the diagnosis of chronic Pseudomonas aeruginosa lung infection in cystic fibrosis. J Med Microbiol 1988; 27:169–177
    [Google Scholar]
  26. Aronoff SC, Stern RC. Serum IgG antibody to outer membrane antigens of Pseudomonas cepacia and Pseudomonas aeruqinosa in cystic fibrosis. J Infect Dis 1988; 157:934–940
    [Google Scholar]
  27. Speert DP, Dimmick JE, Pier GB, Saunders JM, Hancock REW, Kelly N. An immunohistochemical evaluation of Pseudomonas aeruginosa pulmonary infection in two patients with cystic fibrosis. Pediatr Res 1987; 22:743–747
    [Google Scholar]
  28. Allison JS, Dawson M, Drake D, Montie TC. Electrophoretic separation and molecular weight, characterization of Pseudomonas aeruginosa H-antigen flaggellins. Infect Immun 1985; 49:770–774
    [Google Scholar]
  29. Ruths S, Driedijk PC, Weening RS, Out TA. ELISA procedures for the measurement of IgG subclass antibodies to bacterial antigens. J Immunol Methods 1991; 140:67–78
    [Google Scholar]
  30. Hamilton RG. Human IgG subclass measurements in the clinical laboratory. Clin Chem 1987; 33:1707–1725
    [Google Scholar]
  31. Schur PH. IgG subclasses—a review. Ann Allerqy 1987; 58:89–86 99
    [Google Scholar]
  32. Jefferis R, Kumararatne DS. Selective IgG subclass deficiency: quantification and clinical relevance. Clin Exp Immunol 1990; 81:357–367
    [Google Scholar]
  33. Matthews-Greer JM, Robertson DE, Gilleland LB, Gilleland HE. Pseudomonas aeruginosa outer membrane protein F produced in Escherichia coli retains vaccine efficacy. Curr Microbiol 1990; 20:171–175
    [Google Scholar]
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