1887

Abstract

Summary

is associated strongly with severe periodontitis, but little information is available on possible transmission routes of this species. This study evaluaated three DNA-based molecular typing methods for use in epidemiological surveys of . In total, 32 isolates from eight married couples were investigated by: (i) restriction endonuclease analysis (REA) of whole chromosomal DNA; (ii) hybridisation of DNA fragments with ribosomal DNA (ribotyping); and (iii) amplification of DNA by the polymerase chain reaction with arbitrary primers (AP-PCR). The data obtained with the three methods were in broad agreement: In six of the eight couples, the isolates from husband and wife were indistinguishable, but isolates from unrelated individuals showed distinct types with all three methods. For some isolates, minor differences in REA pattern were obtained which could not be correlated with differences in ribotype or AP-PCR type. Ribotyping showed differences between isolates from one individual, which were indistinguishable with the other two methods. The patterns obtained with ribotyping or AP-PCR were simple in comparison to the relatively complex REA patterns. Although all three methods were concordant, AP-PCR was found to be the least time-consuming method. The data support the suggestion that can be transmitted between spouses.

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1993-12-01
2022-09-27
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References

  1. Mayrand D, Holt SC. Biology of asaccharolytic black-pigmented Bacteroides species. Microbiol Rev 1988; 52:134–152
    [Google Scholar]
  2. Slots J, Listgarten M. Bacteroides gingivalis, Bacteroides inter-medius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol 1988; 15:85–93
    [Google Scholar]
  3. van Steenbergen TJM, van Winkelhoff AJ, de Graaff J. Black-pigmented oral anaerobic rods: classification and role in periodontal disease. In: Hamada S, Holt HC, McGhee JR. (eds) Periodontal disease: pathogens and host immune responses Tokyo: Quintessence Publishing Co.; 199141–52
    [Google Scholar]
  4. van Winkelhoff AJ, van Steenbergen TJM, de Graaff J. The role of black-pigmented Bacteroides in human oral infections. J Clin Periodontol 1988; 15:145–155
    [Google Scholar]
  5. van Steenbergen TJM, Petit MDA, Scholte LHM, van der Velden U, de Graaff J. Transmission of Porphyromonas gingivalis between spouses. J Clin Periodontol 1993; 20:304–345
    [Google Scholar]
  6. Petit MDA, van Steenbergen TJM, Scholte LHM, van der Velden U, de Graaff J. Epidemiology and transmission of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans among children and their family members. A report of 4 surveys. J Clin Periodontol 1993 (in press)
    [Google Scholar]
  7. Loos BG, Mayrand D, Genco RJ, Dickenson DP. Genetic heterogeneity of Porphyromonas (Bacteroides) gingivalis by genomic DNA fingerprinting. J Dent Res 1990; 69:1488–1493
    [Google Scholar]
  8. van Steenbergen TJM, van Winkelhoff AJ, van der Velden U, de Graaff J. Taxonomy, virulence and epidemiology of black-pigmented Bacteroides species in relation to oral infections. Infection 1989; 17:194–196
    [Google Scholar]
  9. van Steenbergen TJM, van der Velden U, Abbas F, de Graaff J. Microflora and bacterial DNA restriction enzyme analysis in young adults with periodontitis. J Periodontology 1991; 62:235–241
    [Google Scholar]
  10. Saarela M, Stucki AM, von Troil-Linden B, Alalusua S, Jousimies-Somer H, Asikainen S. Intra- and interindividual comparison of Porphyromonas gingivalis genotypes. FEMS Immunol Microbiol 1993; 6:99–102
    [Google Scholar]
  11. Socransky SS, Martin L. Ribotyping of P. gingivalis and C rectus.. J Dent Res 1992; 71:246 (abstract)
    [Google Scholar]
  12. Menard C, Brousseau R, Mouton C. Application of polymerase chain reaction with arbitrary primer (AP-PCR) to strain identification of Porphyromonas (Bacteroides) gingivalis. FEMS Microbiol Lett 1992; 95:163–168
    [Google Scholar]
  13. Brosius J, Ullrich A, Raker MA et al. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 1981; 6:112–118
    [Google Scholar]
  14. Loos BG, Dyer DW, Genco RJ, Selander RK, Dickinson AP. Natural history and epidemiology of Porphyromonas gingivalis. In: Shah HN, Mayrand D, Genco RJ. (eds) Porphyromonas gingivalis Boca Raton, FL: CRC Press Inc.; 1993 (in press)
    [Google Scholar]
  15. van Steenbergen TJM, van Winkelhoff AJ, de Graaff J. Classification and typing methods of black-pigmented Gram-negative anaerobes. FEMS Immunol Microbiol 1993; 6:83–88
    [Google Scholar]
  16. Grimont F, Grimont PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 1986; 137B:165–175
    [Google Scholar]
  17. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  18. Williams GK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531–6535
    [Google Scholar]
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