Detection of serum and faecal antibodies in haemorrhagic colitis caused by O157 Free

Abstract

Summary

Case of culture-confirmed clinically typical haemorrhagic colitis caused by verocytotoxin-producing (VT) O157 and age- and sex-matched control patients were examined for antibodies to O157. Serum samples from 28 cases and 34 patients in control group 1 were examined for VT- and VT-neutralising antibodies, O157 agglutinating antibodies, and by an enzyme immunoassay (EIA) technique for IgG antibodies against smooth lipopolysaccharide purified from O157 and for IgG antibodies against whole intact O157 cells. Differences between antibody tires were significant when compared by a Wilcoxon two-sample test for O157 agglutinating antibodies (p < 0.05) and IgG antibodies against whole cells (p < 0.001). The whole-cell EIA was used further to examine faecal samples from 93 cases and 47 patients in control group 2 for IgA antibodies. Elevated levels of faecal IgA specific for O157 were found in 59 (63.4%) of 93 cases but in only 10 (21.2%) of 47 control patients (p < 0.001); follow-up faecal samples from five cases all showed marked rises in levels of IgA that appeared to coincide with cessation of excretion of the organism. Detection of specific faecal IgA with a whole-cell EIA, although requiring further evaluation, may be a useful addition to tests currently available for the diagnosis of infection by VT O157.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-6-408
1993-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/6/medmicro-39-6-408.html?itemId=/content/journal/jmm/10.1099/00222615-39-6-408&mimeType=html&fmt=ahah

References

  1. Karmali MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989; 2:15–38
    [Google Scholar]
  2. Smith HR, Rowe B, Gross RJ, Fry NK, Scotland SM. Haemorrhagic colitis and verocytotoxin-producing Escherichia coli in England and Wales. Lancet 1987; 1:1062–1065
    [Google Scholar]
  3. Chapman PA, Wright DJ, Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect 1989; 102:439–445
    [Google Scholar]
  4. Karmali MA, Petrie M, Lim C, Fleming PC, Arbus GS, Lior H. The association between idiopathic hemolytic-uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis 1985; 151:775–782
    [Google Scholar]
  5. Scotland SM, Rowe B, Smith HR, Willshaw GA, Gross RJ. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic-uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25:237–243
    [Google Scholar]
  6. Chapman PA, Siddons CA, Wright DJ, Norman P, Fox J, Crick E. Cattle as a source of verotoxigenic Escherichia coli O157. Vet Rec 1992; 131:323–324
    [Google Scholar]
  7. Chapman PA, Jewes L, Siddons CA, Norman P, George SL. Vero cytotoxin-producing Escherichia coli infections in Sheffield 1985-1989. PHLS Microbiol Digest 1990; 7:163–166
    [Google Scholar]
  8. Ratnam S, March SB, Sprague WD, Severs D, Sullivan RM. Are humans a source of Escherichia coli O157.H7, the agent of hemorrhagic colitis?. N Engl J Med 1986; 315:1612–1613
    [Google Scholar]
  9. Carter AO, Borczyk AA, Carlson JA et al. A severe outbreak of Escherichia coli O157.H7 associated hemorrhagic colitis in a nursing home. N Engl J Med 1987; 317:1496–1500
    [Google Scholar]
  10. March SB, Ratnam S. Sorbitol-MacConkey medium for the detection of Escherichia coli O157.H7 associated with hemorrhagic colitis. J Clin Microbiol 1986; 23:869–872
    [Google Scholar]
  11. Chapman PA, Siddons CA, Zadik PM, Jewes L. An improved selective medium for the isolation of Escherichia coli Ol 57. J Med Microbiol 1991; 35:107–110
    [Google Scholar]
  12. Willshaw GA, Smith HR, Scotland SM, Rowe B. Cloning of genes determining the production of vero cytotoxin by Escherichia coli. J Gen Microbiol 1985; 131:3047–3053
    [Google Scholar]
  13. Jackson MP, Neill RJ, O’Brien AD, Holmes RK, Newland JW. Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933. FEMS Microbiol Lett 1987; 44:109–114
    [Google Scholar]
  14. Karch H, Meyer T. Evaluation of oligonucleotide probes for identification of Shiga-like toxin-producing Escherichia coli. J Clin Microbiol 1989; 26:1180–1186
    [Google Scholar]
  15. Jackson MP. Detection of Shiga toxin-producing Shigella dysenteriae type 1 and Escherichia coli by using polymerase chain reaction with incorporation of digoxigenin-11- dUTP. J Clin Microbiol 1991; 29:1910–1914
    [Google Scholar]
  16. Tarr PI, Neill MA, Clausen CR, Watkins SL, Christie DL, Hickman RO. Escherichia coli O157: H7 and the hemolytic- uremic syndrome: importance of early cultures in establishing the etiology. J Infect Dis 1990; 162:553–556
    [Google Scholar]
  17. Notenboom RH, Borczyk A, Karmali MA, Duncan LM. Clinical relevance of a serological cross-reaction between Escherichia coli 0151 and Brucella abortus. Lancet 1987; 2:745
    [Google Scholar]
  18. Chart H, Scotland SM, Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic-uremic syndrome. J Clin Microbiol 1989; 27:285–290
    [Google Scholar]
  19. Bitzan M, Moebius E, Ludwig K, Muller-Wiefel DE, Heese-mann J, Karch H. High incidence of serum antibodies to Escherichia coli O157 lipopolysaccharide in children with hemolytic-uremic syndrome. JPediatr 1991; 119:380–385
    [Google Scholar]
  20. Chapman PA, Swift DL. A simplified method for detecting the heat-labile enterotoxin of Escherichia coli. J Med Microbiol 1984; 18:399–403
    [Google Scholar]
  21. Westphal O, Jann K. Bacterial lipopolysaccharides - extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 1965; 5:83–91
    [Google Scholar]
  22. Cryz SJ, Cross AS, Sadoff JC, Furer E. Synthesis and characterisation of Escherichia coli 018 O-polysaccharide conjugate vaccines. Infect Immun 1990; 58:373–377
    [Google Scholar]
  23. Karkhanis YD, Zeltner JY, Jackson JJ, Carlo DJ. A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem 1978; 85:595–601
    [Google Scholar]
  24. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning, 2nd ed. Cold Spring Harbour Press; 1989
    [Google Scholar]
  25. Tsai CM, Frasch CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115–119
    [Google Scholar]
  26. Zadik P. An enzyme-linked immunosorbent assay to detect IgG antibodies to cell surface components of Pseudomonas cepacia in patients with cystic fibrosis. Serodiagn Immun- other Infect Dis 1988; 2:349–356
    [Google Scholar]
  27. MacDougall J, Hodson ME, Pitt TL. Antibody response of fibrocystic patients to homologous O-typable and O-defective isolates of Pseudomonas aeruginosa. J Clin Pathol 1990; 43:567–571
    [Google Scholar]
  28. Chapman PA, Rush BA, McLauchlin J. An enzyme immuno assay for detecting Cryptosporidium in faecal and environmental samples. J Med Microbiol 1990; 32:233–238
    [Google Scholar]
  29. Aleksic S, Karch H, Bockemuhl J. A biotyping scheme for Shiga-like (Vero) toxin-producing Escherichia coli O157 and a list of serological cross-reactions between O157 and other gram-negative bacteria. ZblBakt 1992; 276:221–230
    [Google Scholar]
  30. Winsor DK, Mathewson JJ, DuPont HL. Comparison of serum and fecal antibody responses of patients with naturally acquired Shigella sonnei infection. J Infect Dis 1988; 158:1108–1112
    [Google Scholar]
  31. Bartholomeusz RC, Forrest RD, Labrooy JT, Ey PL, Pyle D, Shearman DJ, Rowley D. The serum polymeric IgA antibody response to typhoid vaccination; its relationship to the intestinal response. Immunology 1990; 69:190–194
    [Google Scholar]
  32. Quiding M, Nordstrom I, Kilander A et al. Intestinal immune response in humans. Oral cholera vaccination induces strong intestinal antibody responses and interferon-)’ production and evokes local immunological memory. Clin Invest 1991; 88:143–148
    [Google Scholar]
  33. Freter R, De SP, Mondal A, Shrivastava DL, Sunderman FW. Coproantibody and serum antibody in cholera patients. J Infect Dis 1965; 115:83–87
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-6-408
Loading
/content/journal/jmm/10.1099/00222615-39-6-408
Loading

Data & Media loading...

Most cited Most Cited RSS feed