The antibody response of cattle to the minor 89-kDa outer-membrane protein (OMP) of brucella was measured by indirect ELISA with the purified protein and compared with the antibody response to smooth lipopolysaccharide (S-LPS). Pre-incubating sera with sonicated cell extracts of Escherichia coli prevented the binding of antibodies from uninfected animals to the 89-kDa OMP, suggesting the presence of one or more cross-reactive epitopes on this protein. In cattle infected experimentally with Brucella abortus, the antibody response to the 89-kDa OMP was later and less intense than that to S-LPS. In naturally infected cattle, 68% of animals showing an antibody response to S-LPS also showed an antibody response to the 89-kDa OMP. Results indicate that specific epitopes of the 89-kDa OMP in combination with those of other OMPs could be useful for diagnosis of brucellosis in cattle.
DubrayG.
Antigens of diagnostic significance in Brucella. In:
VergerJM,
PlommetM.
(eds) Brucella melitensis, a CEE seminar The Hague: Martinus Nijhoff Publishing; 1985123–138
CloeckaertA,
JacquesI,
de WergifosseP,
DubrayG,
LimetJN.
Protection against Brucella melitensis or Brucella abortus in mice with immunoglobulin G (IgG), IgA, and IgM monoclonal antibodies specific for a common epitope shared by the Brucella A and M smooth lipopolysac- charides. Infect Immun1992; 60:312–315
LimetJN,
BosserayN,
Garin-BastujiB,
DubrayG,
PlommetM.
Humoral immunity in mice mediated by monoclonal antibodies against the A and M antigens of Brucella. J Med Microbiol1989; 30:37–43
PhillipsM,
DeyoeBL,
CanningPC.
Protection of mice against Brucella abortus infection by inoculation with monoclonal antibodies recognizing Brucella O-antigen. Am J Vet Res1989; 50:2158–2161
JacquesI,
Olivier-BernardinV,
DubrayG.
Induction of antibody and protective responses in mice by Brucella O-polysaccharide-BSA conjugate. Vaccine1991; 9:896–900
WinterAJ,
RoweGE,
DuncanJR et al. Effectiveness of the natural and synthetic complexes of porin and O polysaccharide as vaccines against Brucella abortus in mice. Infect Immun1988; 56:2808–2817
CloeckaertA,
de WergifosseP,
DubrayG,
LimetJN.
Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labelling for electron microscopy and enzyme-linked immunosorbent assay. Infect Immun1990; 58:3980–3987
CloeckaertA,
JacquesI,
BosserayN et al. Protection conferred on mice by monoclonal antibodies directed against outer- membrane-protein antigens of Brucella. J Med Microbiol1991; 34:175–180
DubrayG,
CharriautC.
Evidence of three major polypeptide species and two major polysaccharide species in the Brucella outer membrane. Ann Rech Vet1983; 14:311–318
CloeckaertA,
ZygmuntMS,
de WergifosseP,
DubrayG,
LimetJN.
Demonstration of peptidoglycan-associated Brucella outer-membrane proteins by use of monoclonal antibodies. J Gen Microbiol1992; 138:1543–1550
SowaBA,
KellyKA,
FichtTA,
FreyM,
AdamsLG.
SDS-soluble and peptidoglycan-bound proteins in the outer membrane-peptidoglycan complex of Brucella abortus. Vet Microbiol1991; 27:351–369
Gomez-MiguelMJ,
MoriyonI.
Demonstration of a peptido-glycan-linked lipoprotein and characterization of its trypsin fragment in the outer membrane of Brucella spp. Infect Immun1986; 53:678–684
Gomez-MiguelMJ,
MoriyonI,
LopezJ.
Brucella outer membrane lipoprotein shares antigenic determinants with Escherichia coli Braun lipoprotein and is exposed on the cell surface. Infect Immun1987; 55:258–262
LetessonJJ,
Lostrie-TrussartN,
DepelchinA.
Production d’anticorps monoclonaux specifiques d’isotypes d’imm- unoglobulines. Ann Med Vet1985; 129:131–141
CloeckaertA,
ZygmuntMS,
NicolleJ-C,
DubrayG,
LimetJN.
O-chain expression in the rough Brucella melitensis strain B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. J Gen Microbiol1992; 138:1211–1219
ZygmuntMS,
GilbertFB,
DubrayG.
Purification, characterization, and seroreactivity of a 20-Kilodalton Brucella protein antigen. J Clin Microbiol1992; 30:2662–2667
ZygmuntMS,
MartinJ-C,
DubrayG.
Analysis of immune response: comparison of immunoblots after isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis using cytoplasmic protein extract from Brucella. FEMS Microbiol Lett1990; 70:263–268