1887

Abstract

Summary

A surface protein of was purified in one step by affinity chromatography with bovine submaxillary mucin coupled to agarose. The purified protein, with a mol. wt of 200 kDa and an iso-electric point of pI 6.5, showed haemagglutinating activity for bovine erythrocytes. The haemagglutinin (HA) inhibited the adherence of to a rat lung cell line (L2) and was able to bind to -acetylneuraminic acid. These findings suggest that the HA of is an adhesin.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-5-388
1993-11-01
2023-02-05
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/5/medmicro-39-5-388.html?itemId=/content/journal/jmm/10.1099/00222615-39-5-388&mimeType=html&fmt=ahah

References

  1. Andersson B, Dahmen J, Frejd T. et al. Identification of an active disaccharide unit of a glycoconjugate receptor for pneumococci attaching to human pharyngeal epithelial cells. J Exp Med 1983; 158:559–570
    [Google Scholar]
  2. Andersson B, Porras O, Hanson LA, Lagergárd T, Swanborg-Eden C. Inhibition of attachment of Streptococcus pneumoniae and Haemophilus influenzae by human milk and receptor oligosaccharides. J Infect Dis 1986; 153:232–237
    [Google Scholar]
  3. Jones WG, Freter R. Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infect Immun 1976; 14:240–245
    [Google Scholar]
  4. Krivan HC, Roberts DD, Ginsburg V. Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc/?l-4Gal found in some glycolipids. Proc Nat AcadSci USA 1988; 85:6157–6161
    [Google Scholar]
  5. Lai C-H, Bloomquist C, Liljemark WF. Purification and characterization of an outer membrane protein adhesin from Haemophilus parainfluenzae HP-28. Infect Immun 1990; 58:3833–3839
    [Google Scholar]
  6. Loomes LM, Uemura K, Childs RA. et al. Erythrocyte receptors for Mycoplasma pneumoniae are sialylated oligosaccharides of Ii antigen type. Nature 1984; 307:560–563
    [Google Scholar]
  7. McSweegan E, Walker RI. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun 1986; 53:141–148
    [Google Scholar]
  8. Parkkinen J, Rogers GN, Korhonen T, Dahr W, Firme J. Identification of the O-linked sialyloligosaccharides of glycophorin A as the erythrocyte receptors for S-fimbriated Escherichia coli. Infect Immun 1986; 54:37–42
    [Google Scholar]
  9. Plotkin BJ, Bemis DA. Adherence of Bordetella bronchiseptica to hamster lung fibroblasts. Infect Immun 1984; 46:697–702
    [Google Scholar]
  10. Smit H, Gaastra W, Kamerling JP, Vliegenthart JFG, de Graaf FK. Isolation and structural characterization of the equine erythrocyte receptor for enterotoxigenic Escherichia coli K99 fimbrial adhesin. Infect Immun 1984; 46:578–584
    [Google Scholar]
  11. Ishikawa H, Isayama Y. Effect of antigenic modulation and phase variation on adherence of Bordetella bronchiseptica to porcine nasal epithelial cells. Am J Vet Res 1987; 48:1689–1691
    [Google Scholar]
  12. Ishikawa H, Isayama Y. Bovine erythrocyte-agglutinin as a possible adhesin of Bordetella bronchiseptica responsible for binding to porcine nasal epithelium. J Med Microbiol 1988; 25:205–209
    [Google Scholar]
  13. Reiman DA, Domenighini M, Tuomanen E, Rappuoli R, Falkow S. Filamentous hemagglutinin of Bordetella pertussis: Nucleotide sequence and crucial role in adherence. Proc Nat AcadSci USA 1989; 86:2637–2641
    [Google Scholar]
  14. Tuomanen E, Weiss A. Characterization of two adhesins of Bordetella pertussis for human ciliated respiratory-epithelial cells. J Infect Dis 1985; 152:118–125
    [Google Scholar]
  15. Leininger E, Roberts M, Kenimer JG. Pertactin, an Arg-Gly- Asp-containing Bordetella pertussis surface protein that promotes adherence of mammalian cells. Proc Nat Acad SciUSA 1991; 88:345–349
    [Google Scholar]
  16. Ashworth LA, Robinson A. et al. Agglutinogens and fimbriae of Bordetella pertussis. Tokai J Exp Clin Med 1988; 13:203–210
    [Google Scholar]
  17. Ishikawa H, Isayama Y. Evidence for sialyl glycoconjugates as receptors for Bordetella bronchiseptica on swine nasal mucosa. Infect Immun 1987; 55:1607–1609
    [Google Scholar]
  18. Ravindranath MH, Higa HH, Cooper EL, Paulson JC. Purifi cation and characterization of an O-acetylsialic acid-specific lectin from a marine crab Cancer antennarius. J Biol Chem 1985; 260:8850–8856
    [Google Scholar]
  19. Sakurai Y, Suzuki H, Kobayashi N, Terada E, Takakura A, Kagiyama N. Determination of an antigen suitable for enzyme-linked immunosorbent assay of the antibody to Bordetella bronchiseptica in guinea pigs. Exp Anim 1990; 39:549–555
    [Google Scholar]
  20. Goel MC. New method for the isolation of membranes from Mycoplasma gallisepticum. J Bacteriol 1973; 116:994–1000
    [Google Scholar]
  21. Hovowitz SA, Cassell GH. Detection of antibodies to Myco plasma pulmonis by an enzyme-linked immunosorbent assay. Infect Immun 1978; 22:161–170
    [Google Scholar]
  22. Lowry HO, Rosebrough NJ. et al. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193:265–275
    [Google Scholar]
  23. Laemmli UK. Cleavage of structual proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  24. Catsimpoolas N. Micro isoelectric focusing in polyacrylamide gel columns. Anal Biochem 1968; 26:480–482
    [Google Scholar]
  25. Kimura A, Mountzouros KT, Reiman DA, Falkow S, Cowell JL. Bordetellapertussis filamentous hemagglutinin: Evaluation as a protective antigen and colonization factor in a mouse respiratory infection model. Infect Immun 1990; 58:7–16
    [Google Scholar]
  26. Domenighini M, Reiman D, Capian C. et al. Genetic characterization of Bordetella pertussis filamentous haemagglutinin: a protein processed from an unusually large precursor. Mol Microbiol 1990; 4:787–800
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-5-388
Loading
/content/journal/jmm/10.1099/00222615-39-5-388
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error