1887

Abstract

Summary

Methicillin-resistant (MRSA) isolates from a possible outbreak in Singapore (84) were examined together with all MRSA isolated in Denmark in 1986—1990 (58) and 14 distinct epidemic and 10 distinct single hospital strains from England and Wales. All 84 Singapore isolates were phage typed routinely and 52 isolates were further analysed together with the Danish isolates with an additional set of experimental phages and by lectin typing. The British strains, previously phage typed in the same way, were lectin typed. The following lectins were used: Wheat-term agglutinin, soy-bean agglutinin, tomato lectin and Concanavalin-A. Routine phage typing of the Danish isolates showed that 41 isolates belonged to 19 different types; 17 isolates were non-typable (NT). Addition of experimental phage typing and lectin typing enhanced discrimination to 47 types. The 24 British strains could be divided into nine “lectin types”. Sixty-one of the isolates from Singapore were non-typable by phage typing; the remaining 23 strains belonged to five types. Further examination of 52 isolates with the experimental set of phages and by lectin typing gave 14 closely related types; 48% of these isolates belonged to only two types.

Erratum

An erratum has been published for this content:
Erratum
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-4-305
1993-10-01
2022-08-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/4/medmicro-39-4-305.html?itemId=/content/journal/jmm/10.1099/00222615-39-4-305&mimeType=html&fmt=ahah

References

  1. Chambers HF. Methicillin-resistant staphylococci. Clin Microbiol Rev 1988; 1:173–186
    [Google Scholar]
  2. Kerr S, Kerr GE, Mackintosh CA, Marples RR. A survey of methicillin-resistant Staphylococcus aureus affecting patients in England and Wales. J Hosp Infect 1990; 16:35–48
    [Google Scholar]
  3. Khalifa KI, Heiba AA, Hancock G. Nontypeable bacterio phage patterns of methicillin-resistant Staphylococcus aureus involved in a hospital outbreak. J Clin Microbiol 1989; 27:2249–2251
    [Google Scholar]
  4. Marples RR, Cooke EM. Current problems with methicillin-resistant Staphylococcus aureus. J Hosp Infect 1988; 11:381–392
    [Google Scholar]
  5. Richardson JF, Marples RR. Another strain of methicillin-resistant Staphylococcus aureus epidemic in London. Lancet 1988; 2:748–749
    [Google Scholar]
  6. Blair JE, Williams REO. Phage typing of staphylococci. Bull WHO 1961; 24:771–784
    [Google Scholar]
  7. Richardson JF, Chittasobhon N, Marples RR. Supplementary phages set for the investigation of strains of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1988; 25:67–74
    [Google Scholar]
  8. Rosdahl VT, Knudsen AM. The decline of methicillin resistance among Danish Staphylococcus aureus strains. Infect Control Hosp Epidemiol 1991; 12:83–88
    [Google Scholar]
  9. Debray H, Pierce-Cretel A, Spik G, Montreuil J. Affinity of ten insolubilized lectins towards various glycopeptides with the N-glycosamine linkage and related oligosaccharides. In: Bog-Hansen TC. (ed) Lectins, vol 3 New York: Walter de Gruyter Co.; 1983335–351
    [Google Scholar]
  10. Goldstein IJ, Hayes CE. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 1978; 35:127–340
    [Google Scholar]
  11. Merkle RK, Cummings RD. Relationship of the terminal sequences to the length of poly-N-acetyllactosamine chains in aspargine-linked oligosaccharides from the mouse lymphoma cell line BW5147. Immobilized tomato lectin interacts with high affinity with glycopeptides containing long poly-N-acetyllactosamine chains. J Biol Chem 1987; 262:8179–8189
    [Google Scholar]
  12. Osawa T, Tsuji T. Fractionation and structural assessment of oligosaccharides and glycoproteins by use of immobilized lectins. Am Rev Biochem 1987; 56:21–42
    [Google Scholar]
  13. Slifkin M, Doyle RJ. Lectins and their application in clinical microbiology. Clin Microbiol Rev 1990; 3:197–218
    [Google Scholar]
  14. Jarlov JO, Hansen J-ES, Rosdahl VT, Espersen F. The typing of Staphylococcus epidermidis by a lectin-binding assay. J Med Microbiol 1992; 37:195–200
    [Google Scholar]
  15. Casals JB, Pedersen OG. Tablet sensitivity testing: a comparison of different methods. Acta Pathol Microbiol Scand Sect B 1972; 80:806–816
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-4-305
Loading
/content/journal/jmm/10.1099/00222615-39-4-305
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error