1887

Abstract

Summary

Porphyrin production by the haemin-independent in the diagnostic porphyrin test, which determines the X-factor requirement in spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9—8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.

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/content/journal/jmm/10.1099/00222615-39-4-262
1993-10-01
2022-06-25
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