1887

Abstract

Summary

Eighteen different strains of were all shown to produce an EDTA-sensitive proteinase of 50 kDa that cleaved the heavy chain, but not the light chain, of IgG. Digestion of pure IgG with small amounts of pure proteinase generated Fabc’ and Fab’ fragments; greater amounts generated Fab and Fc fragments that were comparable in size to those generated by pepsin and papain, respectively. Incubation of neutrophils with IgG digested with proteinase or papain resulted in a marked decrease in the respiratory burst activity of the neutrophils that coincided with cleavage of the IgG into Fab and Fc fragments. Analysis of urine from patients with urinary tract infection revealed in many the presence of Fab and Fc fragments of IgG indistinguishable in size from those generated by proteinase. These results indicate that, in urinary tract infections, the proteinase is secreted and cleaves IgG to fragments that have defective immune effector functions, thereby limiting the effectiveness of the immune response.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-3-225
1993-09-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/3/medmicro-39-3-225.html?itemId=/content/journal/jmm/10.1099/00222615-39-3-225&mimeType=html&fmt=ahah

References

  1. Boyle MDP. Bacterial immunoglobulin-binding proteins. Microbiology, chemistry and biology vol. 1 London: Academic Press; 1990
    [Google Scholar]
  2. Langone JJ. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumococci. Ado Immunol 1982; 32:157–252
    [Google Scholar]
  3. Russell-Jones GJ, Gotschlich EC, Blake Milan S. A surface receptor specific for human IgA on group B streptococci possessing the IbC protein antigen. J Exp Med 1984; 160:1467–1475
    [Google Scholar]
  4. Blake M, Holmes KK, Swanson J. Studies on gonococcus infection. XVII IgA-1 cleaving protease in vaginal washings from women with gonorrhea. J Infect Dis 1979; 139:89–92
    [Google Scholar]
  5. Insel RA, Allen PZ, Berkowitz ID. Types and frequency of Haemophilus influenzae IgAl proteases. Semin Infect Dis 1982; 4:225–231
    [Google Scholar]
  6. Plaut AG, Genco RJ, Tomasi TB. Isolation of an enzyme from Streptococcus sanguis which specifically cleaves IgA. J Immunol 1974; 113:S89–S91
    [Google Scholar]
  7. Kilian M, Holmgren K. Ecology and nature of immunoglobulin A1 pro tease-producing streptococci in the human oral cavity and pharynx. Infect Immun 1981; 31:868–873
    [Google Scholar]
  8. Mulks MH, Plaut AG. IgA protease production as a characteristic distinguishing pathogenic from harmless Neisser-iaceae. N Engl J Med 1978; 299:973–976
    [Google Scholar]
  9. Senior BW, Loomes LM, Kerr MA. Microbial IgA proteases and virulence. Rev Med Microbiol 1991; 2:200–207
    [Google Scholar]
  10. Loomes LM, Senior BW, Kerr MA. A proteolytic enzyme secreted by Proteus mirabilis degrades immunoglobulins of the immunoglobulin A1 (IgAl), IgA2, and IgG isotypes. Infect Immun 1990; 58:1979–1985
    [Google Scholar]
  11. Loomes LM, Senior BW, Kerr MA. Proteinases of Proteus spp.: purification, properties and detection in urine of infected patients. Infect Immun 1992; 60:2267–2273
    [Google Scholar]
  12. Senior BW, Loomes LM, Kerr MA. The production and activity in vivo of Proteus mirabilis IgA protease in infections of the urinary tract. J Med Microbiol 1991; 35:203–207
    [Google Scholar]
  13. Senior BW. Typing of Proteus strains by proticine production and sensitivity. J Med Microbiol 1977; 10:7–17
    [Google Scholar]
  14. Senior BW, Leslie DL. Rare occurrence of Proteus vulgaris in faeces: a reason for its rare association with urinary tract infections. J Med Microbiol 1986; 21:139–144
    [Google Scholar]
  15. Greenwood FC, Hunter WM, Glover JS. The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochem J 1963; 89:114–123
    [Google Scholar]
  16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  17. Albrechtsen M, Yeaman GR, Kerr MA. Characterization of the IgA receptor from human polymorphonuclear leucocytes. Immunology 1988; 64:201–205
    [Google Scholar]
  18. Stewart WW, Kerr MA. The specificity of the human neutrophil IgA receptor (FcaR) determined by measurement of chemiluminescence induced by serum or secretory IgAl or IgA2. Immunology 1990; 71:328–334
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-39-3-225
Loading
/content/journal/jmm/10.1099/00222615-39-3-225
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error