1887

Abstract

Summary

type b expresses an inducible siderophore-independent iron-acquisition system that depends on a direct interaction between human transferrin and specific iron-regulated transferrin-binding outer-membrane proteins. To evaluate the importance of this iron-acquisition system amongst haemophili, 156 isolates of spp. (78 commensal isolates and 78 isolates from invasive infections) were examined for their ability to bind transferrin. Of the 78 invasive isolates, all of which were type b, 71 (91%) were capable of binding transferrin, with 57 (73%) binding transferrin constitutively (i.e., even when grown in an iron-sufficient medium). In contrast, only 11 (14%) of the commensal isolates bound transferrin constitutively, with a further 16 (21%) binding transferrin only after growth in an iron-deficient medium. Of the 27 commensal strains that were capable of binding transferrin, 12 were biotype III, 14 were non-typable and one was None of the type b invasive or commensal isolates showed evidence of siderophore production, but 50 (66%) of the remaining 76 commensal isolates appeared to produce an iron chelator. Thus, while not a universal characteristic, detectable transferrin-binding was associated strongly with type b isolates from invasive infections, and was also recognised for the first time in isolates of and .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-3-218
1993-09-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/3/medmicro-39-3-218.html?itemId=/content/journal/jmm/10.1099/00222615-39-3-218&mimeType=html&fmt=ahah

References

  1. Hoiseth SK. The genus Haemophilus. In: Balows A, Triiper HG, Dworkin M, Harder W, Schleifer K-H. (eds) The prokaryotes New York: Springer-Verlag; 19923304–3330
    [Google Scholar]
  2. Turk DC. The pathogenicity of Haemophilus influenzae. J Med Microbiol 1984; 18:1–16
    [Google Scholar]
  3. Albritton WL. Infections due to Haemophilus species other than H. influenzae. Annu Rev Microbiol 1982; 36:199–216
    [Google Scholar]
  4. Otto BR, Verweij-van Vught AMJJ, MacLaren DM. Trans ferrins and heme-compounds as iron sources for pathogenic bacteria. Crit Rev Microbiol 1992; 18:217–233
    [Google Scholar]
  5. Griffiths E. The iron-uptake systems of pathogenic bacteria. In: Bullen JJ, Griffiths E. (eds) Iron and infection: molecular, physiological and clinical aspects Chichester: Wiley; 198769–137
    [Google Scholar]
  6. Williams P, Morton DJ, Towner KJ, Stevenson P, Griffiths E. Utilization of enterobactin and other exogenous iron sources by Haemophilus influenzae H. parainfluenzae and H. paraphrophilus. J Gen Microbiol 1990; 136:2343–2350
    [Google Scholar]
  7. Tsai J, Dyer DW, Sparling PF. Loss of transferrin receptor activity in Neisseria meningitidis correlates with inability to use transferrin as an iron source. Infect Immun 1988; 56:3132–3138
    [Google Scholar]
  8. Schryvers AB. Identification of the transferrin- and lactoferrin-binding proteins in Haemophilus influenzae. J Med Microbiol 1989; 29:121–130
    [Google Scholar]
  9. Ala’Aldeen DA, Davies HA, Wall RA, Borriello SP. The 70 kilodalton iron-regulated protein of Neisseria meningitidis is not the human transferrin receptor. FEMS Microbiol Lett 1990; 69:37–42
    [Google Scholar]
  10. Griffiths E, Stevenson P, Ray A. Antigenic and molecular heterogeneity of the transferrin-binding protein of Neisseria meningitidis. FEMS Microbiol Lett 1990; 69:31–36
    [Google Scholar]
  11. Morton DJ, Williams P. Siderophore-independent acquisition of transferrin-bound iron by Haemophilus influenzae type b. J Gen Microbiol 1990; 136:927–933
    [Google Scholar]
  12. Anderson P, Johnson RB, Smith DH. Human serum activities against Haemophilus influenzae type b. J Clin Invest 1972; 51:31–38
    [Google Scholar]
  13. Holland J, Langford PR, Towner KJ, Williams P. Evidence for in vivo expression of transferrin-binding proteins in Haemophilus influenzae type b. Infect Immun 1992; 60:2986–2991
    [Google Scholar]
  14. Cruickshank R. (ed) Medical microbiology, 11th edn.. Edinburgh: Churchill Livingstone; 1965748
    [Google Scholar]
  15. Kilian M. Haemophilus. In: Lennette EH, Balows A, Hausler WJ, Truant JP. (ed) Manual of clinical microbiology, 3rd edn.. Washington, D.C.: American Society for Microbiology; 1980330–336
    [Google Scholar]
  16. Taylor DC, Cripps AW, Clancy RL et al. Biotypes of Haemophilus parainfluenzae from the respiratory secretions in chronic bronchitis. J Med Microbiol 1992; 36:279–282
    [Google Scholar]
  17. Rogers HJ. Iron-binding catechols and virulence in Escherichia coli. Infect Immun 1973; 7:445–456
    [Google Scholar]
  18. Ely S, Tippett J, Moxon ER. Identification and characterization of a serotype b-specific segment of the Haemophilus influenzae genome. Infect Immun 1989; 57:2926–2928
    [Google Scholar]
  19. Carter GI, Towner KJ, Slack RCB. Detection of TEM beta-lactamase genes by non-isotopic spot hybridization. Eur J Clin Microbiol 1987; 6:406–409
    [Google Scholar]
  20. Moxon ER, Deich RA, Connelly C. Cloning of chromosomal DNA from Haemophilus influenzae. Its use for studying the expression of type b capsule and virulence. J Clin Invest 1984; 73:298–306
    [Google Scholar]
  21. Holland J, Towner KJ, Williams P. Isolation and characterisation of Haemophilus influenzae type b mutants defective in transferrin-binding and iron assimilation. FEMS Microbiol Lett 1991; 77:283–287
    [Google Scholar]
  22. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal Biochem 1987; 160:47–56
    [Google Scholar]
  23. Kroll JS, Ely S, Moxon ER. Capsular typing of Haemophilus influenzae with a DNA probe. Mol Cell Probes 1991; 5:375–379
    [Google Scholar]
  24. Schryvers AB. Characterization of the human transferrin and lactoferrin receptors in Haemophilus influenzae. Mol Microbiol 1988; 2:467–472
    [Google Scholar]
  25. Herrington DA, Sparling PF. Haemophilus influenzae can use human transferrin as a sole source for required iron. Infect Immun 1985; 48:248–251
    [Google Scholar]
  26. Morton DJ, Williams P. Utilization of transferrin-bound iron by Haemophilus species of human and porcine origins. FEMS Microbiol Lett 1989; 65:123–128
    [Google Scholar]
  27. Gonzalez GC, Caamano DL, Schryvers AB. Identification and characterization of a porcine-specific transferrin receptor in Actinobacillus pleuropneumoniae. Mol Microbiol 1990; 4:1173–1179
    [Google Scholar]
  28. Ogunnariwo JA, Cheng C, Ford J, Schryvers AB. Response of Haemophilus somnus to iron limitation: expression and identification of a bovine-specific transferrin receptor. Microb Pathog 1990; 9:397–406
    [Google Scholar]
  29. Ogunnariwo JA, Schryvers AB. Correlation between the ability of Haemophilus paragallinarum to acquire ovotransferrin- bound iron and the expression of ovotransferrin-specific receptors. Avian Dis 1992; 36:655–663
    [Google Scholar]
  30. Schryvers AB, Morris LJ. Identification and characterization of the transferrin receptor from Neisseria meningitidis . Mol Microbiol 1988; 2:281–288
    [Google Scholar]
  31. Stevenson P, Williams P, Griffiths E. Common antigenic domains in transferrin-binding protein 2 of Neisseria meningitidis, Neisseria gonorrhoeae, and Haemophilus influenzae type b. Infect Immun 1992; 60:2391–2396
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-3-218
Loading
/content/journal/jmm/10.1099/00222615-39-3-218
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error