1887

Abstract

Summary

The pathogenicity of oral bacteria was studied by measuring the development of subcutaneous abscesses in mice after infection with and either singly or as co-aggregated pairs. Heat-treated cells were also tested. The pathogenicity of the co-aggregates was examined in various viable and heat-treated combinations of the two bacterial species. More abscesses were formed by than at all the bacterial concentrations tested. Also, abscess formation by co-aggregates of the two strains produced a higher percentage of abscess formation than those caused by infection with pure suspensions of or Co-aggregated cells were more resistant to phagocytosis and killing by neutrophils and Furthermore, in co-aggregates were resistant to killing after engulfment by neutrophils. These results suggest that oral bacteria that are able to co-aggregate may resist phagocytosis, and this ability may be linked with pathogenicity.

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1993-09-01
2024-04-19
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References

  1. Jordan HV, Keyes PH, Bellack S. Periodontal lesions in hamsters and gnotobiotic rats infected with Actinomyces of human origin. J Periodont Res 1972; 7:21–28
    [Google Scholar]
  2. Onderdonk AB, Bartlett JG, Louie T, Sullivan-Seigler N, Gorbach SL. Microbial synergy in experimental intraabdominal abscess. Infect Immun 1976; 13:22–26
    [Google Scholar]
  3. MacDonald JB, Socransky SS, Gibbons RJ. Aspects of the pathogenesis of mixed anaerobic infections of mucous membranes. J Dent Res 1963; 42:529–544
    [Google Scholar]
  4. Mayrand D, McBride BC. Ecological relationships of bacteria involved in a simple, mixed anaerobic infection. Infect Immun 1980; 27:44–50
    [Google Scholar]
  5. Bowden GH, Hardie JM. Commensal and pathogenic Actino myces species in man. In: Sykes G, Skinner FA. (eds) Society for Applied Bacteriology Symposium Series No. 2 New York: Academic Press Inc.; 1973277–299
    [Google Scholar]
  6. Ellen RP. Oral colonization by gram-positive bacteria significant to periodontal disease. In: Genco RJ, Mergenhagen SE. (eds) Host-parasite interactions in periodontal disease Washington, DC: American Society for Microbiology; 1982; 98111
    [Google Scholar]
  7. Loesche WJ, Syed SA. Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score. Infect Immun 1978; 21:830–839
    [Google Scholar]
  8. Syed SA, Loesche WJ. Bacteriology of human experimental gingivitis: effect of plaque age. Infect Immun 1978; 21:821–829
    [Google Scholar]
  9. Jordan HV, Fitzgerald RJ, Stanley HR. Plaque formation and periodontal pathology in gnotobiotic rats infected with an oral actinomycete. Am J Pathol 1965; 47:1157–1167
    [Google Scholar]
  10. Nyvad B, Killian M. Microbiology of the early colonization of human enamel and root surface in vivo. Scand J Dent Res 1987; 95:369–380
    [Google Scholar]
  11. Gibbons RJ, Nygaard M. Interbacterial aggregation of plaque bacteria. Arch Oral Biol 1970; 15:1397–1400
    [Google Scholar]
  12. Bourgeau G, McBride BC. Dextran-mediated interbacterial aggregation between dextran-synthesizing streptococci and Actinomyces viscosus. Infect Immun 1976; 13:1228–1234
    [Google Scholar]
  13. Cisar JO, Kolenbrander PE, Mclntire FC. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun 1979; 24:742–752
    [Google Scholar]
  14. Jones SJ. A special relationship between spherical and filamentous microorganisms in mature human dental plaque. Arch Oral Biol 1972; 17:613–616
    [Google Scholar]
  15. Listgarten MA, Mayo H, Amsterdam M. Ultrastructure of the attachment device between coccal and filamentous microorganisms in “Corn Cob” formations of dental plaque. Arch Oral Biol 1973; 18:651–656
    [Google Scholar]
  16. Kolenbrander PE, Williams BL. Lactose-reversible coaggregation between oral Actinomycetes and Streptococcus sanguis. Infect Immun 1981; 33:95–102
    [Google Scholar]
  17. Mclntire FC, Vatter AE, Baros J, Arnold J. Mechanism of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34. Infect Immun 1978; 21:978–988
    [Google Scholar]
  18. Slack J. The source of infection in actinomycosis. J Bacteriol 1942; 43:193–209
    [Google Scholar]
  19. White JC, Niven CF. Streptococcus SBE: A streptococcus associated with subacute bacterial endocarditis. J Bacteriol 1946; 51:717–722
    [Google Scholar]
  20. Ochiai K, Ikeda T, Shiota T. An improved method for measuring aggregation of certain streptococcal bacteria found in dental plaque. Arch Oral Biol 1983; 28:253–258
    [Google Scholar]
  21. Toris Y, Iwasaki K, Tanaka J, Sonozaki H. Chemotactic factor. In: Experimental methods in immunology. Japanese Society for Immunology 19812609–2616
    [Google Scholar]
  22. Gibbons RJ, Spinell DM. Salivary-induced aggregation of plaque bacteria. In: McHugh WD. (ed) Symposium on dental plaque Wynnewood, PA: E and S Livingstone; 1970207–215
    [Google Scholar]
  23. Gibbons RJ, Fitzgerald RJ. Dextran-induced aggregation of Streptococcus mutans, and its potential role in the formation of microbial dental plaque. J Bacteriol 1969; 98:341–346
    [Google Scholar]
  24. Malamud D, Appelbaum B, Kline R, Golub EE. Bacterial aggregation activity in human saliva: comparisons of bacterial species and strains. Infect Immun 1981; 31:1003–1006
    [Google Scholar]
  25. Brown JR. Human actinomycosis, a study of 181 subjects. Hum Pathol 1973; 4:319–330
    [Google Scholar]
  26. Davenport AA, Cater GR, Schirmer RG. Canine actinomycosis due to Actinomyces viscosus: report of six cases. Vet Med Small Anim Clin 1974; 69:1442–1447
    [Google Scholar]
  27. Engel D, Van Epps D, Clagett J. In vivo and in vitro studies on possible pathogenic mechanisms of Actinomyces viscosus. Infect Immun 1976; 14:548–554
    [Google Scholar]
  28. Brown JR, von Lichtenberg F. Experimental actinomycosis in mice-study of pathogenesis. Arch Pathol 1970; 90:391–402
    [Google Scholar]
  29. Verbrugh HA, Peters R, Peterson PK, Verhoef J. Phagocytosis and killing of staphylococci by human polymorphonuclear and mononuclear leukocytes. J Clin Pathol 1978; 31:539–545
    [Google Scholar]
  30. Passo SA, Tsai CC, McArthur WP, Leifer C, Taichiman NS. Interaction of inflammatory cells and oral microorganisms. IX. The bactericidal effects of human polymorphonuclear leukocytes on isolated plaque microorganisms. J Periodont Res 1980; 15:470–482
    [Google Scholar]
  31. Sandberg AL, Mudrick LL, Cisar JO, Brennan MJ, Mergen-Hagen SE, Vetter AE. Type two fimbrial lectin-mediated phagocytosis of oral Actinomyces spp. by polymorphonuclear leukocytes. Infect Immun 1986; 54:472–476
    [Google Scholar]
  32. Loesche WJ, Syed SA, Schmidt E, Morrison EC. Bacterial profiles of subgingival plaques in periodontitis. J Periodontol 1985; 56:447–456
    [Google Scholar]
  33. Slots J, Listgarten MA. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal disease. J Clin Periodontal 1988; 15:85–93
    [Google Scholar]
  34. Ellen RP, Grove DA. Bacteroides gingivalis vesicles bind to and aggregate Actinomyces viscosus. Infect Immun 1989; 57:1618–1620
    [Google Scholar]
  35. Kolenbrander PE, Celesk RA. Coaggregation of human oral Cytophaga species and Actinomyces israelii. Infect Immun 1983; 40:1178–1185
    [Google Scholar]
  36. Kolenbrander PE, Andersen RN, Holdeman LV. Coaggre gation of oral Bacteroides species with other bacteria: central role in coaggregation bridges and competitions. Infect Immun 1985; 48:741–746
    [Google Scholar]
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