1887

Abstract

Summary

Potassium tellurite was assessed for the selection of verocytotoxigenic (VT) O157. MICs were higher for VT O157 than for other strains of and for spp. MacConkey medium containing sorbitol, tellurite and cefixime (TC-SMAC) permitted the growth of VT O157 and but partially or completely inhibited the growth of 67% of other strains of and all or most strains of other sorbitol-non-fermenting species tested. Of 391 rectal swabs from cattle screened on TC-SMAC medium, 26 yielded isolates of VT O157 whereas sorbitol-MacConkey medium with cefixime and rhamnose yielded only nine isolates. Inclusion of potassium tellurite in sorbitol-MacConkey agar markedly increased the rate of isolation of VT O157 from cattle rectal swabs and may do so for other types of specimen.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-2-155
1993-08-01
2022-05-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/2/medmicro-39-2-155.html?itemId=/content/journal/jmm/10.1099/00222615-39-2-155&mimeType=html&fmt=ahah

References

  1. Karmali MA. Infection by verocytotoxin-producing Escherichia coli . Clin Microbiol Rev 1989; 2:15–38
    [Google Scholar]
  2. Riley LW, Remis RS, Helgerson SD et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med 1983; 308:681–685
    [Google Scholar]
  3. Pai CH, Gordon R, Sims HV, Bryan LE. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157: H7. Ann Intern Med 1984; 101:738–742
    [Google Scholar]
  4. Smith HR, Rowe B, Gross RJ, Fry NK, Scotland SM. Haemorrhagic colitis and verocytotoxin-producing Escherichia coli in England and Wales. Lancet 1987; 1:1062–1065
    [Google Scholar]
  5. Chapman PA, Wright DJ, Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect 1989; 102:439–445
    [Google Scholar]
  6. Karmali MA, Steele BT, Petrie M, Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cyto-toxin and cytotoxin-producing Escherichia coli in stools. Lancet 1983; 1:619–620
    [Google Scholar]
  7. Karmali MA, Petrie M, Lim C, Fleming PC, Arbus GS, Loir H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis 1985; 151:775–782
    [Google Scholar]
  8. Scotland SM, Rowe B, Smith HR, Willshaw GA, Gross RJ. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25:237–243
    [Google Scholar]
  9. Spika JS, Parsons JE, Nordenberg D, Wells JG, Gunn RA, Blake PA. Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O157: H7 in a day care center. J Pediatr 1986; 109:287–291
    [Google Scholar]
  10. Martin ML, Shipman LD, Wells JG et al. Isolation of Escherichia coli O157: H7 from dairy cattle associated with two cases of haemolytic-uraemic syndrome. Lancet 1986; 2:1043
    [Google Scholar]
  11. Chapman PA, Jewes L., Siddons CA, Norman P, George SL. Verotoxin-producing Escherichia coli infections in Sheffield during 1989. PHLS Microbiol Digest 1990; 7:163–166
    [Google Scholar]
  12. Ratnam S, March SB, Sprague WD, Severs D, Sullivan RM. Are humans a source of Escherichia coli O157: H7, the agent of hemorrhagic colitis?. N Engl J Med 1986; 315:1612–1613
    [Google Scholar]
  13. Carter AO, Borczyk AA, Carlson JAK et al. A severe outbreak of Escherichia co//0157: H7-associated hemorrhagic colitis in a nursing home. N Engl J Med 1987; 317:1496–1500
    [Google Scholar]
  14. March SB, Ratnam S. Sorbitol-MacConkey Medium for detection of Escherichia coli O157: H7 associated with hemorrhagic colitis. J Clin Microbiol 1986; 23:869–872
    [Google Scholar]
  15. Chapman PA, Siddons CA, Zadik PM, Jewes L. An improved selective medium for the isolation of Escherichia coli O157. J Med Microbiol 1991; 35:107–110
    [Google Scholar]
  16. Okrend AJG, Rose BE, Lattuada CA. Use of 5-bromo-5-chloro-3-indoxyl-β-D-glucuronide in MacConkey sorbitol agar to aid the isolation of Escherichia coli O157: H7 from ground beef. J Food Protect 1990; 53:941–943
    [Google Scholar]
  17. Okrend AJG, Rose BR, Lattuada CP. Isolation of Escherichia coli O157: H7 using 0157 specific antibody coated magnetic beads. J Food Protect 1992; 55:214–217
    [Google Scholar]
  18. Shimada T, Sakazaki R, Fujimura S, Niwano K, Mishina M, Takizawa K. A new selective, differential agar medium for isolation of Vibrio cholerae 01: PMT (Polymyxin- mannose-tellurite) agar. Jpn J Med Sci Biol 1990; 43:37–41
    [Google Scholar]
  19. Mujibur Rahaman M, Golam Morshed M, Sultanul Aziz KM, Munshi MMH. Improved medium for isolating shigella. Lancet 1986; 1:271–272
    [Google Scholar]
  20. Chapman PA. Evaluation of a commercial latex slide test for identifying Escherichia coli O157. J Clin Pathol 1989; 42:1109–1110
    [Google Scholar]
  21. Walter EG, Taylor DE. Plasmid-mediated resistance to tellurite: expressed and cryptic. Plasmid 1992; 27:52–64
    [Google Scholar]
  22. Tomas JM, Kay WW. Tellurite susceptibility and non-plasmid- mediated resistance in Escherichia coli. Antimicrob Agents Chemother 1986; 30:127–131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-2-155
Loading
/content/journal/jmm/10.1099/00222615-39-2-155
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error