Mortality rates amongst mice with endogenous septicaemia caused by isolates from various clinical sources Free

Abstract

Summary

Mice that had been treated with cyclophosphamide and ampicillin were fed with . These procedures induced an endogenous septicaemia under conditions mimicking the pathophysiology of the disease in man. This model was used to compare the mortality rates in mice infected with isolates from various clinical sources. Mortality rates in mice given isolates from blood cultures had a broad range (0—100%), but the mean rate was significantly higher than with isolates from other infection sites. Moreover, blood isolates persisted in the intestines of mice after oral inoculation, whereas most isolates from other sources were gradually eliminated. Most isolates from blood culture produced significantly higher levels of exotoxin A and total proteases than isolates from other infection sites. Amongst the blood isolates, all but one of the lethal strains produced large quantities of exotoxin A or total proteases or both. Taken together, the results suggest that the ability of to adhere to the intestinal tract and to produce high levels of exo-enzymes may contribute to the development of fatal septicaemia.

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1993-08-01
2024-03-28
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References

  1. Kreger BE, Craven DE, McCabe WR. Gram-negative bacteremia. IV. Re-evaluation of clinical features and treatment in 612 Patients. Am J Med 1980; 68:344–355
    [Google Scholar]
  2. Pizzo PA, Young LS. Limitations of current antimicrobial therapy in the immunosuppressed host: looking at both sides of the coin. Am J Med 1984; 76:Suppl 3A101–110
    [Google Scholar]
  3. Schimpff SC, Young VM, Green WH et al. Origin of infection in acute nonlymphocytic leukemia. Ann Intern Med 1972; 77:707–714
    [Google Scholar]
  4. Tancrede CH, Andremont AO. Bacterial translocation and gram-negative bacteraemia in patients with hematological malignancies. J Infect Dis 1979; 152:99–103
    [Google Scholar]
  5. Baltch AL, Griffin PE. Pseudomonas aeruginosa bacteremia; a clinical study of 75 patients. Am J Med Sci 1977; 274:119–129
    [Google Scholar]
  6. Pollack M, Young LS. Protective activity of antibodies to exotoxin A and lipopolysaccharide at the onset of Pseudomonas aeruginosa septicaemia in man. J Clin Invest 1979; 63:276–286
    [Google Scholar]
  7. Hirakata Y, Tomono K, Tateda K et al. Role of bacterial association with Kupffer cells in occurrence of endogenous systemic bacteraemia. Infect Immun 1991; 59:289–294
    [Google Scholar]
  8. Bjorn MJ, Vasil ML, Sadoff JC, Iglewski BH. Incidence of exotoxin production by Pseudomonas species. Infect Immun 1977; 16:362–366
    [Google Scholar]
  9. Cross AS, Sadoff JC, Iglewski BH, Sokol PA. Evidence for the role of toxin A in the pathogenesis of infection with Pseudomonas aeruqinosa in humans. J Infect Dis 1980; 14:538–546
    [Google Scholar]
  10. Liu PV. Extracellular toxins of Pseudomonas aeruginosa . J Infect Dis 1974; 130:Suppl:94–99
    [Google Scholar]
  11. Cryz SJ, Iglewski BH. Production of alkaline protease by Pseudomonas aeruginosa . J Clin Microbiol 1980; 12:131–133
    [Google Scholar]
  12. Collins HH, Cross AS, Dobek A, Opal SM, McClain JB, Sadoff JC. Oral ciprofloxacin and a monoclonal antibody to lipopolysaccharide protect leukopenic rats from lethal infection with Pseudomonas aeruginosa . J Infect Dis 1989; 159:1073–1082
    [Google Scholar]
  13. Hirakata Y, Kaku M, Tomono K et al. Efficacy of erythromycin lactobionate for treating Pseudomonas aeruginosa bacteremia in mice. Antimicrob Agents Chemother 1992; 36:1198–1203
    [Google Scholar]
  14. Bjorn MJ, Sokol PA, Iglewski BH. Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. J Bacteriol 1979; 138:193–200
    [Google Scholar]
  15. Iglewski BH, Sadoff JC. Toxin inhibitors of protein synthesis: production, purification, and assay of Pseudomonas aeruginosa toxin A. Methods Enzymol 1979; 60:780–793
    [Google Scholar]
  16. Hirakata Y, Kaku M, Mizukane R et al. Potential effects of erythromycin on host defence systems and virulence of Pseudomonas aeruginosa. Antimicrob Agents Chemother 1992; 36:1922–1927
    [Google Scholar]
  17. Woods DE, Schaffer MS, Rabin HR, Campbell GD, Sokol PA. Phenotypic comparison of Pseudomonas aeruginosa strains isolated from a variety of clinical sites. J Clin Microbiol 1986; 24:260–264
    [Google Scholar]
  18. Stinson MW, Hayden C. Secretion of phospholipase C by Pseudomonas aeruginosa. Infect Immun 1979; 25:558–564
    [Google Scholar]
  19. Berka RM, Gray GL, Vasil ML. Studies of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa. Infect Immun 1981; 34:1071–1074
    [Google Scholar]
  20. Richet H, Escande MC, Marie JP, Zittoun R, Lagrange PH. Epidemic Pseudomonas aeruginosa serotype 016 bacteremia in hematology-oncology patients. J Clin Microbiol 1989; 27:1992–1996
    [Google Scholar]
  21. Griffith SJ, Nathan C, Selander RK et al. The epidemiology of Pseudomonas aeruginosa in oncology patients in a general hospital. J Infect Dis 1989; 160:1030–1036
    [Google Scholar]
  22. Correa CMC, Tibana A, Gontijo Filho PP. Vegetables as a source of infection with Pseudomonas aeruginosa in a university and oncology hospital of Rio de Janeiro. J Hosp Infect 1991; 18:301–306
    [Google Scholar]
  23. Jackson RJ, Smith SD, Wadowsky RM, DePudyt L, Rowe MI. The effect of E. coli virulence on bacterial translocation and systemic sepsis in the neonatal rabbit model. J Pediatr Surg 1991; 26:483–486
    [Google Scholar]
  24. Gerke JR, Magliocco MV. Experimental Pseudomonas aeruginosa infection of the mouse cornea. Infect Immun 1971; 3:209–216
    [Google Scholar]
  25. Iglewski BH, Burns RP, Gipson IK. Pathogenesis of comeal damage from Pseudomonas exotoxin A. Invest Ophthalmol Vis Sci 1977; 16:73–76
    [Google Scholar]
  26. Kreger AS, Griffin OK. Physicochemical fractionation of extracellular cornea-damaging proteases of Pseudomonas aeruginosa . Infect Immun 1974; 9:828–834
    [Google Scholar]
  27. Ohman DE, Burns RP, Iglewski BH. Comeal infections in mice with toxin A and elastase mutant of Pseudomonas aeruginosa . J Infect Dis 1980; 142:547–555
    [Google Scholar]
  28. Tillotson JR, Lerner AM. Characteristics of non-bacteremic pseudomonas pneumonia. Ann Intern Med 1968; 68:295–307
    [Google Scholar]
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