1887

Abstract

Summary

The routine use of ELISA and complement fixation tests in the diagnosis of suspected clinical cases of hydatid disease was evaluated. In the ELISA test, dialysed and filtered sheep cyst fluid was used as antigen and two positive cut-off points—+3SD and + 2SD of the mean absorbance values of the control sera—were evaluated. The predictive values of ELISA tests were 82% and 90% for positive tests, and 86% and 82% for negative tests, respectively with the two cut-off points. In a population survey of blood donors and veterinary workers in Powys, 4% and 8%, respectively, had ELISA values above the lower cut-off point. However, it would not be appropriate to use the same test for diagnostic population screening in Wales since the predictive value of the test is likely to be very low in this setting. Serological surveys with the ELISA may be of use in monitoring the progress of the South Powys Hydatid Control Programme. The use of cumulative percentages was found to be a useful method of comparing whole distributions of results in different populations.

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1993-07-01
2022-10-01
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References

  1. Palmer SR, Biffin AHB. The changing incidence of human hydatid disease in England and Wales. Epidemiol Infect 1987; 996:93–700
    [Google Scholar]
  2. Dottorini S, Tassi C, Badelli F. E.L.I.S.A. (Enzyme-linked immunosorbent assay) for diagnosis of human hydatid disease. Boll 1st Sieroter Milan 1981; 60:137–143
    [Google Scholar]
  3. Anon Man, dogs and hydatid disease. Lancet 1987; 1:21–22
    [Google Scholar]
  4. Stallbaumer MF, Clarkson MJ, Bailey JW, Pritchard JE. The epidemiology of hydatid disease in England and Wales. J Hyg 1986; 96:121–127
    [Google Scholar]
  5. Walters TMH. Echinococcus/hydatidosis and the South Powys control scheme. J Small Animal Practice 1986; 27:693–703
    [Google Scholar]
  6. Schantz PM, Kagan IG. Echinococcosis. In: Houba V. (ed) Immunological investigations of tropical parasite disease Edinburgh: Churchill Livingstone; 1980
    [Google Scholar]
  7. Varela-Diaz VM, Coltorti EA. Human hydatidosis immuno-diagnostic techniques. Series scientific technical monographs, No. 7. Buenos Aires, Pan American Zoonosis Center, Pan American Health Organization
    [Google Scholar]
  8. McLaren ML, Lillywhite JE, Dunne DW, Doenhoff MJ. Serodiagnosis of human Schistosoma mansoni infections: enhanced sensitivity and specificity in ELISA using fraction containing S. mansoni egg antigens and ar . Trans R Soc Trop Med Hyg 1981; 75:72–79
    [Google Scholar]
  9. Bradstreet CMP, Taylor CED. Technique of complement fixation test applicable to the diagnosis of virus diseases. Monthly bulletin of the Ministry of Health and PHLS 1962; 21:96
    [Google Scholar]
  10. Robertson L, Farrell ID, Hinchcliffe PM, Quaife RA. Bench-book on Brucella. PHLS Monograph series; 1980
    [Google Scholar]
  11. Rickard MD, Honey RD, Brumley JL, Mitchell GF. Serological diagnosis and post-operative surveillance of human hydatid disease. II. The enzyme-linked immunosorbent assay (ELISA) using various antigens. Pathology 1984; 16:211–215
    [Google Scholar]
  12. Rogan MT, Craig PS, Zeyhle E, Romig T, Libans GM, Deshan L. Evaluation of a rapid dot-ELISA as a field test for the diagnosis of cystic hydatid disease. Trans R Soc Trop Med Hyg 1991; 85:773–777
    [Google Scholar]
  13. Eckert J, Gemmell MA, Soulsby EJL. FAO/UNEP/WHO guidelines for surveillance, prevention and control of echinococcosis/hydatidosis. Geneva: World Health Organization; 1981
    [Google Scholar]
  14. Bonifacino R, Malgor R, Barbeito R. Seroprevalence of Echinococcus granulosus infection in a Uraguayan rural human population. Trans R Soc Trop Med Hyg 1991; 85:769–772
    [Google Scholar]
  15. Howells CHL, Taylor RJ. Hydatid disease in Mid Wales. J Clin Pathol 1980; 33:701
    [Google Scholar]
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