1887

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Volume 39, Issue 1

Serum IgG and sputum IgA antibody to core lipopolysaccharide antigen from in patients with cystic fibrosis Free

Abstract

Summary

The immunological response of cystic fibrosis (CF) patients to lipopolysaccharide (LPS) antigens of was investigated. Enzyme-linked immunosorbent assays (ELISA) with either whole cells or extracted core LPS from a clinical isolate of as antigen were used to measure serum IgG and sputum IgA anti- antibodies. The ELISA with core LPS distinguished nine CF patients colonised by from nine age- and sex-matched non-colonised CF patients. The rate of increase of anti- IgG antibodies after bacteriologically proven colonisation varied in individual patients: In some patients the first isolation of was preceded or accompanied by a two-to-four-fold rise in anti- LPS IgG titres. Absorption studies and immunoblot analysis of serum from patients colonised with demonstrated that a significant component of the anti- core LPS antibodies was specific for and did not react with the core LPS of . Immunoblotting also illustrated that there may be a degree of core heterogeneity between different isolates of . Detection of LPS specific antibodies in serum (IgG) and sputum (IgA) from CF patients is recommended to assist the identification of colonisation in CF patients.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1993
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1993-07-01
2024-12-02
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Serum IgG and sputum IgA antibody to core lipopolysaccharide antigen from Pseudomonas cepacia in patients with cystic fibrosis
J. Med. Microbiol. 39, 39 (1993); https://doi.org/10.1099/00222615-39-1-39
/content/journal/jmm/10.1099/00222615-39-1-39
/content/journal/jmm/10.1099/00222615-39-1-39
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