1887

Abstract

Summary

Surface topography of used ( > 12 months) and unused CAPD catheters was studied by scanning electronmicroscopy (SEM) and confocal laser scanning microscopy (CLSM). Microbial biofilm was observed on all used catheters. Disruption and removal of the attached biofilm revealed extensive pitting of the catheter surface and scoring within the catheter pores. Similar, though less extensive, surface defects were present in unused catheters. Examination by CLSM, with software specific to the determination of surface topography, showed used catheters to have a surface microrugosity greater than that of unused catheters (p > 0·0005). Adherence studies with radiolabelled demonstrated increased adherence to used than to unused catheters (p > 0·0005) after 48 h. However, when catheters were pre-treated with spent dialysate there was a substantial reduction in bacterial adherence to either catheter and no significant difference in adherence to used and unused catheters. Surface microrugosity of CAPD catheters increases during use but is unlikely to be an important factor in bacterial adherence

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-6-411
1993-06-01
2022-08-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/6/medmicro-38-6-411.html?itemId=/content/journal/jmm/10.1099/00222615-38-6-411&mimeType=html&fmt=ahah

References

  1. Mawhinney WM, Adair CG, Gorman SP. Development and treatment of peritonitis in continuous ambulatory peritoneal dialysis. Int J Pharm Pract 1991; 1:10–18
    [Google Scholar]
  2. Mawhinney WM, Adair CG, Gorman SP. Examination of microbial biofilm on peritoneal catheters by electron and confocal laser scanning microscopy. Proceedings of the 10 th Pharmaceutical Technology ConferenceBologna, Italy 1991; 2652–660
    [Google Scholar]
  3. Gorman SP. Microbial adherence and biofilm production. In Denyer SP, Hugo WB. eds Mechanisms of action of chemical biocides: their study and exploitation. The Society for Applied Bacteriology Technical Series No. 27. Blackwell Scientific Publications; Oxford: 1991:271–295
    [Google Scholar]
  4. Schmitt DD, Bandyk DF, Pequet AJ, Malangoni MA, Towne JB. Mucin production by Staphylococcus epidermidis. A virulence factor promoting adherence to vascular grafts. Arch Surg 1986; 121:89–95
    [Google Scholar]
  5. Dickinson GM, Bisno AL. Infections associated with indwelling devices: concepts of pathogenesis. Infections associated with intramuscular devices. Antimicrob Agents Chemother 1989; 33:597–601
    [Google Scholar]
  6. Dinen P. The effect of suture material in the development of vascular infection. Vase Surg 1977; 11:29–33
    [Google Scholar]
  7. Muller WA, Cohn LH, Schoen FJ. Infection within a degenerated Starr-Edwards silicone rubber poppet in the aortic valve position. Am J Cardiol 1984; 54:1146
    [Google Scholar]
  8. Fessia SL, Amirana O, Carr KL. Biofilm formation on commercially available plastic tubings. Adv Perit Dial 1988; 4:253–256
    [Google Scholar]
  9. Ludlam HA, Noble WC, Marples RR, Philips I. The evaluation of a typing scheme for coagulase-negative staphylococci suitable for epidemiological studies. J Med Microbiol 1989; 30:161–165
    [Google Scholar]
  10. Gorman SP, McCafferty DF, Woolfson AD, Jones DS. Reduced adherence of microorganisms to human mucosal epithelial cells following treatment with Taurolin, a novel antimicrobial agent. J Appl Bacteriol 1987; 62:315–320
    [Google Scholar]
  11. Holmes CJ, Evans RC, Vonesh E. Application of an empirically derived growth curve model to characterize Staphylococcus epidermidis biofilm development on silicone elastomer. Biomaterials 1989; 10:625–629
    [Google Scholar]
  12. Woolfson AD, Gorman SP, McCafferty DF, Jones DS. On the statistical evaluation of adherence assays. J Appl Bacteriol 1987; 63:147–151
    [Google Scholar]
  13. Diaz-Bianco J, Clawson RC, Roberson SM, Sanders CB, Pramanik AK, Herbst JJ. Electron microscopic evaluation of bacterial adherence to polyvinyl chloride endotracheal tubes used in neonates. Crit Care Med 1989; 17:1335–1340
    [Google Scholar]
  14. Wilkins KM, Martin GP, Hanlon GW, Marriott C. The influence of critical surface tension and microrugosity on the adhesion of bacteria to polymer monofilaments. Int J Pharm 1989; 57:1–7
    [Google Scholar]
  15. Gaylarde CC, Videla HA. Localised corrosion induced by a marine vibrio. Int Biodeter 1987; 23:91–104
    [Google Scholar]
  16. Holah JT, Thorpe RH. Cleanability in relation to bacterial retention on unused and abraded domestic sink materials. J Appl Bacteriol 1990; 69:599–608
    [Google Scholar]
  17. Carballo J, Ferreiros CM, Criado MT. Importance of ex perimental design in the evaluation of the influence of proteins in bacterial adherence to polymers. Med Microbiol Immunol 1991; 180:149–155
    [Google Scholar]
  18. Martinez-Martinez L, Pascual A, Perea EJ. Kinetics of ad herence of mucoid and non-mucoid Pseudomonas aeruginosa to plastic catheters. J Med Microbiol 1991; 34:7–12
    [Google Scholar]
  19. Muller E, Takeda S, Goldmann DA, Pier GB. Blood proteins do not promote adherence of coagulase-negative staphylococci to biomaterials. Infect Immun 1991; 59:3323–3326
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-6-411
Loading
/content/journal/jmm/10.1099/00222615-38-6-411
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error