1887

Abstract

Summary

A carbohydrate-rich and immunobiologically active component, M1Av, was prepared from an M-1 endo--acetylmuramidase digest of cell walls of ATCC 19246 by CM Sephadex C-25 and Sephadex G-100 chromatography. M1Av stimulated thioglycolate-induced peritoneal macrophages from C3H/HeN and C3H/HeJ mice to release cell-free tumour necrosis factor (TNF) and interleukin (IL)-6, and a cell-associated thymocyte activating factor, probably IL-1. An intravenous (i.v.) injection of M1Av induced increased levels of TNF and IL-6 in the serum of C3H/HeN mice that had been primed with 100 µg of muramyldipeptide (MDP) i.v. However, M1Av did not induce TNF release in C3H/HeJ mice similarly primed with MDP. Simultaneous administration of M1Av (100 κg, i.v.) and galactosamine (18 mg, intraperitoneally) killed C3H/HeN, but not C3H/HeJ mice. M1Av was shown to be practically free of endotoxin by the test. These findings indicate that the solubilised cell-wall carbohydrate moiety induced inflammatory cytokines both and .

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1993-06-01
2024-04-14
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References

  1. Ellen RP. Oral colonization by gram-positive bacteria sig nificant to periodontal disease. In Genco RJ, Mergen-hagen SE. eds Host-parasite interactions in periodontal diseases. Washington DC: American Society for Micro-biology; 1982:98–111
    [Google Scholar]
  2. Ellen RP. Genus Actinomyces and other filamentous bacteria. In Newman MG, Niesengard R. eds Oral microbiology and immunology. Philadelphia, WB: Saunders Co; 1988173–182
    [Google Scholar]
  3. Page RC. Gingivitis. J Clin Periodontal 1986; 13:345–355
    [Google Scholar]
  4. Aukhil I, Lopatin DE, Syed SA, Morrison EC, Kowalski CJ. The effects of periodontal therapy on serum antibody (IgG) levels to plaque microorganisms. J Clin Periodontol 1988; 15:544–550
    [Google Scholar]
  5. Klausen B, Hougen HP, Fiehn N-E. Increased periodontal bone loss in temporarily B lymphocyte-deficient rats. J Periodont Res 1989; 24:384–390
    [Google Scholar]
  6. Chen P, Farrar JJ, Genco RJ. Immunological properties of Actinomyces viscosus: comparison of blastogenic and adjuvant activities. Infect Immun 1980; 28:212–219
    [Google Scholar]
  7. Clagett J, Engel D, Chi E. In vitro expression of immuno globulin M and G subclasses by murine B lymphocytes in response to a polyclonal activator from Actinomyces. Infect Immun 1980; 29:234–243
    [Google Scholar]
  8. Lopatin DE, Mangan DF, Horner IS. Cells involved in the mitogen-induced helper function which facilitates the blastogenic response to Actinomyces viscosus . Clin Immunol Immunopathol 1981; 19:394–405
    [Google Scholar]
  9. Metzger Z, , Hof Teld JT, Charon J, Mergenhagen SE. Sup pression of lymphocyte and fibroblast proliferation by Actinomyces racavwi-activated murine macrophages. J Periodont Res 1987; 22:456–460
    [Google Scholar]
  10. Gaegauf-Zollinger R, Burckhardt JJ, Gmiir R, Guggenheim B. Cell-mediated cytotoxicity against rat fibroblasts induced by Actinomyces viscosus . Infect Immun 1982; 37:710–719
    [Google Scholar]
  11. Hamada S, Okahashi N, Kimura S et al. Novel antigens of oral Actinomyces species prepared from a cell wall enzyme lysate. Jpn J Med Sci Biol 1982; 35:171–181
    [Google Scholar]
  12. Kimura S, Hamada S, Torii M et al. Lymphoid cell responses to bacterial cell wall components: murine B-cell responses to a purified cell wall moiety of Actinomyces . Scand J Immunol 1983; 17:313–322
    [Google Scholar]
  13. Tracey KJ, Cerami A. Cachectin/tumor necrosis factor and other cytokines in infectious disease. Curr Opin Immunol 1989; 1:454–461
    [Google Scholar]
  14. Obayashi T, Tamura H, Tanaka S et al. A new chromogenic endotoxin-specific assay using recombined limulus coagulation enzymes and its clinical applications. Clin Chim Acta 1985; 149:55–65
    [Google Scholar]
  15. Galanos C, Liideritz O, Westphal O. Preparation and properties of a standardized lipopolysaccharide from Salmonella abortus equi (Novo-Pyrexal). Zentralbl Bakteriol Hyg Abt 1 Orig A 1979; 243:226–244
    [Google Scholar]
  16. Matsuda T, Hirano T, Kishimoto T. Establishment of an interleukin 6 (IL 6)/B cell stimulatory factor 2-dependent cell line and preparation of anti-IL 6 monoclonal antibodies. Eur J Immunol 1988; 18:951–956
    [Google Scholar]
  17. Galanos C, Freudenberg MA, Reutter W. Galactosamine- induced sensitization to the lethal effects of endotoxin. Proc Natl Acad Sci USA 1979; 76:5939–5943
    [Google Scholar]
  18. Dunn OJ. Multiple comparisons among means. J Am Statist Ass 1961; 56:52–64
    [Google Scholar]
  19. Hamada S, McGhee JR, Kiyono H, Torii M, Michalek SM. Lymphoid cell responses to bacterial cell wall components: mitogenic responses of murine B cells to Streptococcus mutans carbohydrate antigens. J Immunol 1981; 126:2279–2283
    [Google Scholar]
  20. Torii M, , Mc Ghee JR, Koopman WJ, Hamada S, Michalek SM. Lymphoid cell responses to bacterial cell wall components: polyclonal and immune responses of murine B cells to Streptococcus mutans carbohydrate antigens. J Immunol 1981; 127:2106–2112
    [Google Scholar]
  21. Morisaki I, Kimura S, Torii M et al. Cell wall preparation consisting of group A carbohydrate and peptidoglycan moieties from Streptococcus pyogenes activates murine B lymphocytes. Immunobiology 1985; 170:293–304
    [Google Scholar]
  22. Kimura S, Kiyono H, Beagley KW et al. Streptococcal serotype carbohydrate represents a novel class of type 2 antigen which is T-independent. J Immunol 1987; 138:4387–4394
    [Google Scholar]
  23. Kimura S, Kiyono H, Michalek SM, Hamada S, McGhee JR. Haptenated streptococcal antigens elicit either T cell- dependent type 1 or T cell-independent type 2 immune responses. Immunobiol 1987; 174:146–161
    [Google Scholar]
  24. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477
    [Google Scholar]
  25. Takada H, Kotani S. Immunopharmacological activities of synthetic muramy 1-pep tides. In Stewart-Tull DES, Davies M. eds Immunology of the bacterial cell envelope. Chichester: John Wiley and Sons Inc; 1985119–152
    [Google Scholar]
  26. Nagao S, Akagawa KS, Yamada K, Yagawa K, Tokunaga T, Kotani S. Lack of response of murine peritoneal macro-phages to in vitro activation by muramyl dipeptide (MDP). I. Macrophage activation by MDP is species dependent. Microbiol Immunol 1990; 34:323–335
    [Google Scholar]
  27. Tsutsui O, Kokeguchi S, Matsumura T, Kato K. Relationship of the chemical structure and immunobiological activities of lipoteichoic acid from Streptococcus faecalis (Entero- coccus hirae) ATCC 9790. FEMS Microbiol Lett 1991; 76:211–218
    [Google Scholar]
  28. Ikeda-Fujita T, Kotani S, Tsujimoto M et al. A novel immunomodulator derived from Mycobacterium bovis BCG which holds many bioactivities in common with endotoxins. Jpn J Bacteriol 1987; 42:597–602
    [Google Scholar]
  29. Kotani S, Nagao A, Tamura T et al. Purification and endotoxin like bioactivities of a novel amphiphile from Myco-bacterium bovis BCG. In Masihi KN, Lange W. eds Immunotherapeutic prospects of infectious diseases. Berlin: Springer-Verlag; 199019–36
    [Google Scholar]
  30. Takada H, Tamura M, Kawabata Y et al. Responses of C3H/HeN and C3H/HeJ mice to cytokine-inducing activities of bacterial components without endotoxicity. Jpn J Bacteriol 1992; 47:202
    [Google Scholar]
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