1887

Abstract

Summary

flagellar sheaths were isolated by sucrose density-gradient centrifugation and analysed by electronmicroscopy, SDS-PAGE and gas-liquid chromatography. Electronmicroscopy of thin sections of flagella showed an internal electron-dense filament and a surrounding flagellar sheath with the typical bilayer structure of a membrane. The flagellar filaments could be disintegrated by acid treatment and the resulting isolated flagellar sheaths formed vesicles, sometimes with characteristic structures. Centrifugation of flagellar preparations after acid treatment resulted in the enrichment of flagellar sheaths in the pellet. SDS-PAGE analysis of the pellet showed a reduction of the flagellin band and a number of protein bands of 150, 76, 67, 65, 53, 51, 49, 29·5, 18, 17 and 16 kDa. However, there were no major protein bands characteristic for the sheath. Differences between the protein profiles of Sarkosyl-insoluble membranes and flagellar sheaths appeared in the lower M range of 30-14 kDa. Major fatty acids of isolated flagellar sheaths were C 14:0, C 19:0 cyc, C 18:0, and the LPS-specific fatty acids 3-OH C 16:0 and 3-OH C 18:0. The results demonstrate that the flagellar sheaths of are membranes and contain LPS and proteins.

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1993-05-01
2024-03-29
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References

  1. Peterson WL. Helicobacter pylori and peptic ulcer disease. N Engl J Med 1991; 324:1043–1048
    [Google Scholar]
  2. Blaser MJ. Epidemiology and pathophysiology of Campylo bacter pvlori infections. Rev Infect Dis 1990; 12: Suppl 1 S99–S106
    [Google Scholar]
  3. Goodwin CS, Armstrong JA. Microbiological aspects of Helico bacter pvlori (Campylobacter pylori) . Eur J Clin Microbiol Infect Dis 1990; 9:1–13
    [Google Scholar]
  4. Dick JD. Helicobacter (Campylobacter)pylori: a new twist to an old disease. Annu Rev Microbiol 1990; 44:249–269
    [Google Scholar]
  5. Buck GE. Campylobacter pylori and gastroduodenal disease. Clin Microbiol Rev 1990; 3:1–12
    [Google Scholar]
  6. Parsonnet J, Friedman GD, Vandersteen DP et al. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 1991; 325:1127–1131
    [Google Scholar]
  7. Nomura A, Stemmermann GN, Chyou P-H, Kato I, Perez-Perez GI, Blaser MJ. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med 1991; 325:1132–1136
    [Google Scholar]
  8. Eaton KA, Brooks CL, Morgan DR, Krakowka S. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect Immun 1991; 59:2470–2475
    [Google Scholar]
  9. Eaton KA, Morgan DR, Krakowka S. Campylobacter pylori virulence factors in gnotobiotic piglets. Infect Immun 1989; 57:1119–1125
    [Google Scholar]
  10. Leying H, Suerbaum S, Geis G, Haas R. Cloning and genetic characterization of a Helicobacter pylori flagellin gene. Mol Microbiol 1992; 6:2863–2874
    [Google Scholar]
  11. Geis G, Leying H, Suerbaum S, Mai U, Opferkuch W. Ultrastructure and chemical analysis of Campylobacter pylori flagella. J Clin Microbiol 1989; 27:436–441
    [Google Scholar]
  12. Kostrzynska M, Betts JD, Austin JW, Trust TJ. Identification, characterization, and spatial localization of two flagellin species in Helicobacter pylori flagella. J Bacteriol 1991; 173:937–946
    [Google Scholar]
  13. Luke CJ, Kubiak E, Cockayne A, Elliott TS, Penn CW. Identification of flagellar and associated polypeptides of Helicobacter (formerly Campylobacter) pylori. FEMS Microbiol Lett 1990; 59:225–230
    [Google Scholar]
  14. Follett EAC, Gordon J. An electron microscope study of Vibrio flagella. J Gen Microbiol 1963; 32:235–239
    [Google Scholar]
  15. Fuerst JA, Hayward AC. The sheathed flagellum of Pseudo-monas stizolobii . J Gen Microbiol 1969; 58:239–245
    [Google Scholar]
  16. Seidler RJ, Starr MP. Structure of the flagellum of Bdellovibrio bacteriovorus. J Bacteriol 1968; 95:1952–1955
    [Google Scholar]
  17. Vandamme P, Falsen E, Rossau R et al. Revision of Campylo bacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposals of Arcobacter gen. nov. Int J Syst Bacteriol 1991; 41:88–103
    [Google Scholar]
  18. Fuerst JA, Perry JW. Demonstration of lipopolysaccharide on sheathed flagella of Vibrio cholerae O:1 by protein A-gold immunoelectron microscopy. J Bacteriol 1988; 170:1488–1494
    [Google Scholar]
  19. Hranitzky KW, Mulholland A, Larson AD, Eubanks ER, Hart LT. Characterization of a flagellar sheath protein of Vibrio cholerae . Infect Immun 1980; 27:597–603
    [Google Scholar]
  20. Thomashow LS, Rittenberg SC. Isolation and composition of sheathed flagella from Bdellovibrio bacteriovorus 109J. J Bacteriol 1985; 163:1047–1054
    [Google Scholar]
  21. Jones DM, Curry A. The ultrastructure of Campylobacter pylori. In Rathbone BJ, Heatley RV. eds Campylobacter pylori and gastroduodenal disease. Oxford: Blackwell Scientific Publications; 198948–59
    [Google Scholar]
  22. Achtman M, Mercer A, Kusecek B et al. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun 1983; 39:315–335
    [Google Scholar]
  23. Markwell MAK, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87:206–210
    [Google Scholar]
  24. Smith PK, Krohn RI, Hermanson GT et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985; 150:76–85
    [Google Scholar]
  25. Lugtenberg B, Meijers J, Peters R, , van der Hoek P, van Alphen L. Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K12 into four bands. FEBSLett 1975; 58:254–258
    [Google Scholar]
  26. Gmeiner J, Martin HH. Phospholipid and lipopolysaccharide in Proteus mirabilis and its stable protoplast L-form: difference in content and fatty acid composition. Eur J Biochem 1976; 67:487–494
    [Google Scholar]
  27. Geis G, Leying H, Suerbaum S, Opferkuch W. Unusual fatty acid substitution in lipids and lipopolysaccharides of Helicobacter pylori . J Clin Microbiol 1990; 28:930–932
    [Google Scholar]
  28. Hu LT, Mobley HL. Purification and N-terminal analysis of urease from Helicobacter pylori. Infect Immun 1990; 58:992–998
    [Google Scholar]
  29. Warren JR, Marshall B. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1983; 1:1273–1275
    [Google Scholar]
  30. Goodwin CS, Armstrong JA, Chilvers T et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb, nov., respectively. Int J Syst Bacteriol 1989; 39:397–405
    [Google Scholar]
  31. Han Y-H, Smibert RM, Krieg NR. Occurrence of sheathed flagella in Campylobacter cinaedi and Campylobacter fen- nelliae. Int J Syst Bacteriol 1989; 39:488–490
    [Google Scholar]
  32. Paster BJ, Lee A, Fox JG et al. Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int J Syst Bacteriol 1991; 41:31–38
    [Google Scholar]
  33. Aizawa SI, Dean GE, Jones CJ, Macnab RM, Yamaguchi S. Purification and characterization of the flagellar hook- basal body complex of Salmonella typhimurium . J Bacteriol 1985; 161:836–849
    [Google Scholar]
  34. Kupper J, Wildhaber I, Gao Z, Baeuerlein E. Basal-body-associated disks are additional structural elements of the flagellar apparatus isolated from Wolinella succinogenes . J Bacteriol 1989; 171:2803–2810
    [Google Scholar]
  35. Logan SM, Trust TJ. Molecular identification of surface protein antigens of Campylobacter jejuni. Infect Immun 1983; 42:675–682
    [Google Scholar]
  36. Fuerst JA. Bacterial sheathed flagella and the rotary motor model for the mechanism of bacterial motility. J Theor Biol 1980; 84:761–774
    [Google Scholar]
  37. Richardson K, Nixon L, Mostow P, Kaper JB, Michalski J. Transposon-induced non-motile mutants of Vibrio cholerae . J Gen Microbiol 1990; 136:717–725
    [Google Scholar]
  38. Richardson K. Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: analysis of motility mutants in three animal models. Infect Immun 1991; 59:2727–2736
    [Google Scholar]
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