%0 Journal Article %A Kunin, C. M. %A Tong, H. H. %A Maher, W. E. %T Naturally-occurring, osmo-remedial variants of Escherichia coli %D 1993 %J Journal of Medical Microbiology, %V 38 %N 3 %P 216-221 %@ 1473-5644 %R https://doi.org/10.1099/00222615-38-3-216 %I Microbiology Society, %X Summary Two clones of Escherichia coli O27:K1:H31 and O2:H7, isolated from patients with urinary tract infection or bacteraemia, failed to grow in a synthetic minimal medium (MM) of low osmolality. They were considered to be osmo-remedial because they grew well when sufficient amounts of NaCl, mannitol or sucrose were added to raise the osmolality of the medium to > 300 mOsm/kg. The defect could also be corrected by nicotinamide or its precursors quinolinic and aspartic acids. Each clone had a unique DNA restriction enzyme profile, fimbriae and antibiotic susceptibility patterns. The osmo-remedial variants were unstable and underwent phenotypic modulation to form mixtures with osmo-tolerant forms when grown in MM. They tended to form satellites of small colonies around large colonies of osmo-tolerant cells on MM agar plates. The penicillin method of Davis was used to separate the two forms. Nicotinamide induced the expression of ompF when the osmo-remedial strains were grown under conditions of low osmolality. It is possible that the variants are defective in the synthesis of membrane-derived oligosaccharides or outer-membrane proteins, but this has yet to be determined. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-38-3-216