To assess the role of diarrhoeagenic in Southern Spain, faecal samples from 135 patients with diarrhoea and 40 healthy subjects from Seville, Andalusia, were investigated. In this prospective study, enterovirulent were identified by hybridisation with five non-radioactive DNA probes specific for enterotoxigenic (ETEC), enteropathogenic (EPEC) and verocytotoxin-producing (VTEC). Probe-positive strains were isolated from four patients (3%) with diarrhoea and from none of the healthy controls. Two patients harboured ETEC and two patients had EPEC probepositive strains in their faeces. No VTEC were isolated during this study. spp. were the most frequently identified enteric pathogens, accounting for 10% of the cases, followed by (3%) and diarrhoeagenic (3%). This study indicates that enterovirulent play a modest role in the aetiology of diarrhoea among the indigenous population of Southern Spain.


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