1887

Abstract

Summary

The microparticle capture enzyme immuno-assay (MEIA) is an automated system for measuring specific antibody by interaction with antigen-coated particles. An MEIA method for detecting toxoplasma-specific IgM was compared with established reference methods. The MEIA had an acceptable level of sensitivity and reproducibility and was easier to perform than conventional tests, but it required expensive, dedicated equipment and, in our study, false positive results were recorded with 7% of samples. MEIA could be used to investigate immunocompetent patients with suspected toxoplasmosis but positive findings should be confirmed by an alternative form of assay. The technique is not suitable for the investigation of neonates, for which a more sensitive method is required.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-1-34
1993-01-01
2023-02-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/1/medmicro-38-1-34.html?itemId=/content/journal/jmm/10.1099/00222615-38-1-34&mimeType=html&fmt=ahah

References

  1. Araujo FG, Remington JS. Toxoplasmosis in immunocom promised patients. Ear J Clin Microbiol 1987; 6:1–2
    [Google Scholar]
  2. Fleck DG. Diagnosis of toxoplasmosis. J Clin Pathol 1989; 42:191–193
    [Google Scholar]
  3. Joynson DHM, Payne RA, Balfour AH, Prestage ES, Fleck DG, Chessum BS. Five commercial enzyme linked immunosorbent assay kits for toxoplasma specific IgM antibody. J Clin Pathol 1989; 42:653–657
    [Google Scholar]
  4. Holliman RE. The diagnosis of toxoplasmosis. Serodiag Immunother Infect Dis 1990; 4:83–93
    [Google Scholar]
  5. Schaefer LE, Dyke JW, Meglio FD, Murray PR, Crafts W, Niles AC. Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMX automated analyzer. J Clin Microbiol 1989; 27:2410–2413
    [Google Scholar]
  6. Siegel C, Goodreau S, Johnston S, Wellens K. Comparison of an automated microparticle enzyme immunoassay (Abbott’s IMX) for the determination of immunoglobulin G to rubella virus and Toxoplasma gondii with El A and latex agglutination for rubella and IFA and complement fixation for toxoplasmosis. Abstract V-12, Annual Meeting of the American Society for Microbiology 1990435
    [Google Scholar]
  7. Payne RA, Joynson DHM, Balfour AH. et al. Public Health Laboratory Service enzyme linked immunosorbent assay for detecting toxoplasma specific IgM antibody. J Clin Pathol 1987; 40:276–281
    [Google Scholar]
  8. Duffy KT, Wharton PJ, Johnson JD, New L, Holliman RE. Assessment of an immunoglobulin-M immunosorbent agglutination assay (ISAGA) for detecting toxoplasma specific IgM. J Clin Pathol 1989; 42:1291–1295
    [Google Scholar]
  9. Johnson J, Duffy K, New L, Holliman RE, Chessum BS, Fleck DG. Direct agglutination test and other assays for measuring antibodies to Toxoplasma qondii. J Clin Pathol 1989; 42:536–541
    [Google Scholar]
  10. Safford JW, Abbott GG, Craine MC, MacDonald RG. Auto mated microparticle enzyme immunoassays for IgG and IgM antibodies to Toxoplasma gondii. J Clin Pathol 1991; 44:238–242
    [Google Scholar]
  11. Holliman RE, Johnson JD. The post-natal serodiagnosis of congenital toxoplasmosis. Serodiag Immunother Infect Dis 1989; 3:323–327
    [Google Scholar]
  12. Luyasu F, Tennsted B, Robert A, Bertrand M, Debruyere M. Serology of toxoplasmosis by MEIA (IMX) method comparison with ISAGA, EIG and IF. Abstract 58 B53 VIIth International Congress Parasitology; 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-38-1-34
Loading
/content/journal/jmm/10.1099/00222615-38-1-34
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error