@article{mbs:/content/journal/jmm/10.1099/00222615-38-1-29, author = "Boukadida, J. and De Montalembert, M. and Lenoir, G. and Scheinmann, P. and VĂ©ron, M. and Berche, P.", title = "Molecular epidemiology of chronic pulmonary colonisation by Pseudomonas aeruginosa in cystic fibrosis", journal= "Journal of Medical Microbiology", year = "1993", volume = "38", number = "1", pages = "29-33", doi = "https://doi.org/10.1099/00222615-38-1-29", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-38-1-29", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary The epidemiology of pulmonary colonisation by Pseudomonas aeruginosa was studied in 21 patients with cystic fibrosis (CF) by field inversion gel electrophoresis. DraI-DNA restriction patterns were analysed for 187 P. aeruginosa isolates from these patients. The results revealed that the strains present in individual patients varied during the course of chronic colonisation; the emergence of new strains often was associated with periods of antibiotic therapy. Patients often were colonised by more than one strain (two or three strains were present in 54% of the patients) and the strains obtained from unrelated patients were highly heterogeneous, in contrast to those isolated from a pair of twins. These results demonstrate the heterogeneity and variability of P. aeruginosa isolates in the pulmonary flora of chronically infected CF patients.", }