1887

Abstract

Summary

Dot-blot analysis of whole-cell suspensions of meningococci showed that 81% of B:15:P1.16 strains from patients reacted with a monoclonal antibody (MAb) against subtype P1.7. The remaining strains, which did not react on dot-blots or in ELISA, demonstrated the P1.7 subtype epitope on immunoblots after denaturation of the cells with sodium dodecyl sulphate. The monomeric class 1 proteins of the two P1.16 subtype variants had slightly different mol. wts, but bound the P1.7 antibody equally well. These results were explained by a deletion of three codons in the gene encoding the first variable region of the P1.16 class 1 protein. The deletion accounted for the non-exposure of the P1.7 epitope on native cells. Other patient strains, with subtypes P1.3, P1.9 or without any known subtype, also showed a binding site for the P1.7 MAb, which became available only after denaturation. Demonstration of inaccessible epitopes may have consequences for subtype designations and vaccine development.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-38-1-23
1993-01-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/38/1/medmicro-38-1-23.html?itemId=/content/journal/jmm/10.1099/00222615-38-1-23&mimeType=html&fmt=ahah

References

  1. Frasch CE, Zollinger WD, Poolman JT. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev Infect Dis 1985; 7:504–510
    [Google Scholar]
  2. Zollinger WD, Moran EE, Connelly H, Mandrell RE, Brandt B. Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningtidis and their use in serotyping. Infect Immun 1984; 46:260–266
    [Google Scholar]
  3. Abdillahi H, Poolman JT. Neisseria meningitidis group B serosubtyping using monoclonal antibodies in whole-cell ELISA. Microb Pathog 1988; 4:27–32
    [Google Scholar]
  4. Abdillahi H, Poolman JT. Definition of meningococcal class 1 OMP subtyping antigens by monoclonal antibodies. FEMS Microbiol Immunol 1988; 47:139–144
    [Google Scholar]
  5. Ashton FE, Ryan JA, Diena BB, Zollinger WD. Monoclonal antibody analysis of meningococcal disease strains isolated in Canada. In: Poolman JT. et al. (eds) Gonococci and meningococci Dordrecht: Kluwer Academic Publishers; 198815–18
    [Google Scholar]
  6. Calain P, Poolman J, Zollinger W. et al. Serological study of meningococcal isolates in Switzerland and France 1980–1986. Eur J Clin Microbiol Infect Dis 1988; 7:788–791
    [Google Scholar]
  7. Poolman JT, Abdillahi H. Outer membrane protein sero subtyping of Neisseria meningitidis. Eur J Clin Microbiol Infect Dis 1988; 7:291–292
    [Google Scholar]
  8. Ashton FE, Mancino L, Ryan AJ, Poolman JT, Abdillahi H, Zollinger WD. Serotypes and subtypes of Neisseria meningitidis serogroup B strains associated with meningococcal disease in Canada 1977-1989. Can J Microbiol 1991; 37:613–617
    [Google Scholar]
  9. Wedege E, Hoiby EA, Rosenqvist E, Froholm LO. Serotyping and subtyping of Neisseria meningitidis isolates by coagglutination, dot-blotting and ELISA. J Med Microbiol 1990; 31:195–201
    [Google Scholar]
  10. McGuinness BT, Clarke IN, Lambden PR. et al. Point mutation in meningococcal porA gene associated with increased endemic disease. Lancet 1991; 337:514–517
    [Google Scholar]
  11. Hitchcock PJ. Unified nomenclature for pathogenic Neisseria species. Clin Microbiol Rev 19892 Suppl S64–S65
    [Google Scholar]
  12. Barlow AK, Heckels JE, Clarke IN. The class 1 outer membrane protein of Neisseria meningitidis: gene sequence and structural and immunological similarities to gonococcal porins. Mol Microbiol 1989; 3:131–139
    [Google Scholar]
  13. Maiden MCJ, Suker J, McKenna AJ, Bygraves JA, Feavers IM. Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis. Mol Microbiol 1991; 5:727–736
    [Google Scholar]
  14. Tommassen J, Vermeij P, Struyvé M, Benz R, Poolman JT. Isolation of Neisseria meningitidis mutants deficient in class 1 (PorA) and class 3 (PorB) outer membrane proteins. Infect Immun 1990; 58:1355–1359
    [Google Scholar]
  15. McGuinness B, Barlow AK, Clarke IN. et al. Deduced amino acid sequences of class 1 protein (PorA) from three strains of Neisseria meningitidis. Synthetic peptides define the epitopes responsible for serosubtype specificity. J Exp Med 1990; 171:1871–1882
    [Google Scholar]
  16. van der Ley P, Heckels JE, Virji M, Hoogerhout P, Poolman JT. Topology of outer membrane porins in pathogenic Neisseria spp. Infect Immun 1991; 59:2963–2971
    [Google Scholar]
  17. Wedege E, Caugant DA, Froholm LO. Epitopes on the meningococcal class 1 protein. In: Achtman A. et al. (eds) Neisseriae 1990 Berlin: Walter de Gruyter and Co; 1991153–158
    [Google Scholar]
  18. Holten E. Serotypes of Neisseria meningitidis isolated from patients in Norway during the first six months of 1978. J Clin Microbiol 1979; 9:186–188
    [Google Scholar]
  19. Wedege E, Caugant DA, Froholm LO, Zollinger WD. Char acterization of serogroup A and B strains of Neisseria meningitidis with serotype 4 and 21 monoclonal antibodies and by multilocus enzyme electrophoresis. J Clin Microbiol 1991; 29:1486–1492
    [Google Scholar]
  20. Abdillahi H, Poolman JT. Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. FEMS Microbiol Lett 1987; 48:367–371
    [Google Scholar]
  21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  22. Selander RK, Caugant DA, Ochman H, Musser JM, Gilmour MN, Whittam TS. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51:873–884
    [Google Scholar]
  23. Caugant DA, Bol P, Hoiby EA, Zanen HC, Froholm LO. Clones of serogroup B Neisseria meningitidis causing systemic disease in the Netherlands, 1958-1986. J Infect Dis 1990; 162:867–874
    [Google Scholar]
  24. Kapperud G, Nesbakken T, Aleksic S, Mollaret HH. Com parison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia entero-colitica isolates. J Clin Microbiol 1990; 28:1125–1131
    [Google Scholar]
  25. Embley TM. The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 1991; 13:171–174
    [Google Scholar]
  26. Mandrell RE, Zollinger WD. Use of a zwitterionic detergent for the restoration of the antibody-binding capacity of elec-troblotted meningococcal outer membrane proteins. J Immunol Methods 1984; 67:1–11
    [Google Scholar]
  27. Wedege E, Bryn K, Froholm LO. Restoration of antibody binding to blotted meningococcal outer membrane proteins using various detergents. J Immunol Methods 1988; 113:51–59
    [Google Scholar]
  28. Caugant DA, Froholm LO, Bovre K. et al. Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease. Proc Natl Acad Sci USA 1986; 83:4927–4931
    [Google Scholar]
  29. Klebba PE, Benson SA, Bala S. et al. Determinants of OmpF porin antigenicity and structure. J Biol Chem 1990; 265:6800–6810
    [Google Scholar]
  30. Bentley A, Klebba PE. Effect of lipopolysaccharide structure on reactivity of antiporin monoclonal antibodies with the bacterial cell surface. J Bacterial 1988; 170:1063–1068
    [Google Scholar]
  31. Pàques M, Teppema JS, Beuvery EC, Abdillahi H, Poolman JT, Verkleij AJ. Accessibility of gonococcal and meningococcal surface antigens: immunogold labeling for quantitative electron microscopy. Infect Immun 1989; 57:582–589
    [Google Scholar]
  32. Caugant DA, Froholm LO, Selander RK, Bovre K. Sul fonamide resistance in Neisseria meningitidis isolates of clones of the ET-5 complex. APMIS 1989; 97:425–428
    [Google Scholar]
  33. Saukkonen K, Leinonen M, Abdillahi H, Poolman JT. Com parative evaluation of potential components for group B meningococcal vaccine by passive protection in the infant rat and in-vitro bactericidal assay. Vaccine 1989; 7:325–328
    [Google Scholar]
  34. Mandrell RE, Zollinger WD. Human immune response to meningococcal outer membrane protein epitopes after natural infection or vaccination. Infect Immun 1989; 57:1590–1598
    [Google Scholar]
  35. Wedege E, Froholm LO. Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting. Infect Immun 1986; 51:571–578
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-38-1-23
Loading
/content/journal/jmm/10.1099/00222615-38-1-23
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error