@article{mbs:/content/journal/jmm/10.1099/00222615-37-6-420, author = "Abath, F. G. C. and Almeida, A. M. P. and Ferreira, L. C. S.", title = "Identification of surface-exposed Yersinia pestis proteins by radio-iodination and biotinylation", journal= "Journal of Medical Microbiology", year = "1992", volume = "37", number = "6", pages = "420-424", doi = "https://doi.org/10.1099/00222615-37-6-420", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-37-6-420", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary When whole cells (stationary phase) of Yersinia pestis strain EV76 were radiolabelled with Iodogen and 131I, 16 major and 10 minor surface-exposed outer membrane proteins (OMPs) were identified. Labelling with N-hydroxysuccinimidyl 6-biotinylamino-hexanoate (biotin X-NHS) resulted in a complex protein profile detectable after blotting and developing with peroxidase-conjugated avidin. Y. pestis cell fractionation revealed that biotin X-NHS labelled not only OMPs but also proteins of inner cell compartments. Therefore, radiolabelling was the more reliable technique for identifying the OMPs of Y. pestis. ", }