1887

Abstract

Summary

Micro-enzyme-linked immunosorbent assay (Micro-ELISA) systems were developed and evaluated for the detection of circulating (free or immune-complexed) hydatid antigens in the sera of patients with hydatidosis, by employing monospecific antibodies to hydatid-specific antigens of 8-kDa and 116-kDa. Fifteen (75%) of 20 sera from patients with hydatidosis had both 8-kDa and 116-kDa antigens freely circulating in their sera while three and two samples, respectively, had only 8-kDa or 116-kDa antigen. All the surgically confirmed cases of hydatidosis had detectable levels of both 8-kDa and 116-kDa circulating immune complexes in glycine HCl-treated sera. However, none of the sera from control subjects (patients with cysticercosis, ascariasis, ancylostomiasis, hymenolepiasis, amoebic liver abscess or viral hepatitis) had any detectable level of either type of circulating specific antigen. These results suggest that the demonstration of either 8- or 116-kDa antigen(s) in free or immune-complex form could confirm the diagnosis of hydatidosis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-6-396
1992-12-01
2024-02-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/6/medmicro-37-6-396.html?itemId=/content/journal/jmm/10.1099/00222615-37-6-396&mimeType=html&fmt=ahah

References

  1. Rickard M. D., Lightowlers M. W. Immunodiagnosis of hydatid disease In Thompson RCA (ed) The biology of Echinococcus and hydatid disease. London: George Allen and Unwin; 1986217–249
    [Google Scholar]
  2. Schantz P. M., Gottstein B. Echinococcosis (hydatidosis). In Walls K. F., Schantz P. M. (eds) Immunodiagnosis of parasitic disease Orlando: Academic Press; 198669–107
    [Google Scholar]
  3. Schantz P. M., Van Den Bossche H., Eckert J. Chemotherapy for larval echinococcosis in animals and humans: report of a workshop. Z Parasit 1982; 67:5–26
    [Google Scholar]
  4. Bekhti A., Schaaps J.-P., Capron M., Dessaint J.-P., Santoro F., Capron A. Treatment of hepatic hydatid disease with mebendazole: preliminary results in four cases. BMJ 1977; 2:1047–1051
    [Google Scholar]
  5. Morris D. L., Dykes P. W., Dickson B., Marriner S. E., Bogan J. A., Burrows F. G. O. Albendazole in hydatid disease. BMJ 1983; 286:103–104
    [Google Scholar]
  6. Morris D. L., Dykes P. W., Marriner S. E. Albendazole—objective evidence of response in human hydatid disease. JAMA 1985; 253:2053–2057
    [Google Scholar]
  7. DeRosa F., Teggi A. Treatment of Echinococcus granulosus hydatid disease with albendazole. Ann Trop Med Parasitol 1990; 84:467–472
    [Google Scholar]
  8. Matossian R. M. The immunological diagnosis of human hydatid disease. Trans R Soc Trop Med Hyg 1977; 71:101–104
    [Google Scholar]
  9. Tassi C., Dottorini S., Scalise G., Geranio N. Echinococcusgranulosus’, diagnosis of human hydatid disease by the indirect haemagglutination reaction with antigen from hydatid fluid and scoleces. bit J Parasitol 1981; 11:85–88
    [Google Scholar]
  10. Kagan I. G. Serodiagnosis of parasitic disease. In Rase N. R., Friedman H., Fahey J. L. (eds) Manual of clinical laboratory immunology 3rd edn Washington, D. C.: American Society for Microbiology; 1986567–587
    [Google Scholar]
  11. Coltorti E. A., Fernandez E., Guamera E., Lago J., Iriarte J. Field evaluation of an enzyme immunoassay for detection of asymptomatic patients in a hydatid control programe. Am J Trop Med Hyg 1988; 38:603–607
    [Google Scholar]
  12. Njeruh F. M., Gathuma J. M., Okelo G. B. A. Tumboh-Oeri A. G. Diagnosis of human hydatid disease in surgically-confirmed cases by the use of the indirect haemagglutination test based on a thermo-stable lipoprotein and on unfractional hydatid cyst fluid. Ann Trop Med Parasitol 1989; 83:299–303
    [Google Scholar]
  13. Craig P. S. Detection of specific circulating antigen, immune complexes and antibodies in human hydatidosis from Turkana (Kenya) and Great Britain, by enzyme-immunoassay. Parasite Immunol 1986; 8:171–188
    [Google Scholar]
  14. Rickard M. D., Honey R. D., Brumley J. L., Mitchell G. F. Serological diagnosis and post-operative surveillance of human hydatid disease II. The enzyme-linked immunosorbent assays (ELISA) using various antigens. Pathology 1984; 16:211–215
    [Google Scholar]
  15. Olivo A., Plancarte A., Flisser A. Presence of antigen B from Taenia solium cysticercus in other platyhelminthes. Int J Parasitol 1988; 18:543–545
    [Google Scholar]
  16. Shepherd J. C., McManus D. P. Specific and cross-reactive anti gens of Echinococcus granulosus hydatid cyst fluid. Mol Biochem Parasitol 1987; 25:143–154
    [Google Scholar]
  17. Larralde C., Montoya R. M., Sciutto E., Diaz M. L., Govezensky T., Coltroli E. Deciphering western blot of tapeworm antigens (Taenia solium, Echinococcus granulosus and Taenia crassiceps) reacting with sera from neurocysticercosis and hydatid disease patients. Am J Trop Med Hyg 1989; 40:282–290
    [Google Scholar]
  18. Richard-Lenoble D., Smith M. D., Loisy M., Verroust P. J. Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and the detection of circulating immune complexes. Ann Trop Med Parasitol 1978; 72:553–560
    [Google Scholar]
  19. D’Amelio R., Pontesilli O., Palmisano L. Detection and partial characterization of circulating immune complexes in hydatid disease. J Clin Microbiol 1983; 18:1021–1026
    [Google Scholar]
  20. Gottstein B. An immunoassay for the detection of circulating antigens in human echinococcosis. Am J Trop Med Hyg 1984; 33:1185–1191
    [Google Scholar]
  21. Craig P. S., Nelson G. S. The detection of circulating antigen in human hydatid disease. Ann Trop Med Parasitol 1984; 78:219–227
    [Google Scholar]
  22. Pezzella M., Galli C., Delia S. Fractionation and characterization of hydatid fluid antigens with identification of an antigen similar to human serum albumin. Trans R Soc Trop MedHyg 1984; 78:821–826
    [Google Scholar]
  23. Kanwar J. R., Kaushik S. P., Sawhney I. M. S., Kamboj M. S., Mehta S. K., Vinayak V. K. Specific antibodies in serum of patients with hydatidosis recognised by immunoblotting. J Med Microbiol 1992; 36:46–51
    [Google Scholar]
  24. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265–275
    [Google Scholar]
  25. Hudson L., Hay F. C. Practical immunology. 2nd edn Oxford: Blackwell Scientific Publications; 1980
    [Google Scholar]
  26. Hofnagle J. H. Gastroenterology (The liver). In Kaubrich W. S., Kaber M. H., Roth J. L. A., Schaffner F. (eds) Acute viral hepatitis clinical features, laboratory findings and treatment 4th edn vol 5 London: WB Saunders Company; 19852856–2901
    [Google Scholar]
  27. Galen R. S., Gambino S. R. Beyond Normality: the predictive value and efficiency of medical diagnoses. New York: John Wiley and Sons; 197530–40
    [Google Scholar]
  28. Hira P. R., Bahr G. M., Shweiki H. M., Behbehani K. An enzyme-linked immunoabsorbent assay using an arc S antigen for the diagnosis of cystic hydatid disease. Ann Trop Med Parasitol 1990; 84:157–162
    [Google Scholar]
  29. Karavodin L. M., Ash L. R. Circulating immune complexes in experimental filariasis. Clin Exp Immunol 1980; 40:312–317
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-6-396
Loading
/content/journal/jmm/10.1099/00222615-37-6-396
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error