1887

Abstract

Summary

Serological evidence of infection with verotoxin-producing (VTEC) was sought in 28 patients suffering from haemolytic uraemic syndrome (HUS) and 25 age- and sex-matched controls. ELISA was used to detect anti-lipopolysaccharide (LPS) antibodies to strains O157, O111, O26 and NCTC 10418, a non-VTEC strain, and O1. Sera from 19 of the HUS patients but from none of the 25 controls had significant antibody levels to the verotoxin-producing bacteria. Sera from 13 patients reacted with only one LPS of the four verotoxin-producing bacteria; sera from six reacted with more than one LPS antigen but not with LPS of NCTC 10418. Paired sera taken 2–3 weeks apart were obtained from 20 HUS patients; 14 of these had high levels of antibody in the acute phase sample. Analysis of antibody levels in the convalescent sera showed that one patient had an increase, one was unchanged and 12 patients had a decrease in antibody to the verotoxin-producing bacteria.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-6-364
1992-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/6/medmicro-37-6-364.html?itemId=/content/journal/jmm/10.1099/00222615-37-6-364&mimeType=html&fmt=ahah

References

  1. Gasser C., Gautier E., Mannre S. Hamolytisch-uramische Syndrome: Bilaterale Nierenrin-dennekrosen bie akuten Erwobenen hamolytischen Anamien. Schweiz Med Wochenschr 1955; 85:905–909
    [Google Scholar]
  2. Srivastava R. N. Pediatric nephrology problems in India. Ped Nephrol 1987; 1:238–244
    [Google Scholar]
  3. Karmali M. A., Petrie M., Steele B. T., Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cyto-toxin and cytotoxin-producing Escherichia coli in stools. Lancet 1983; 1:619–620
    [Google Scholar]
  4. Karmali M. A., Petrie M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic-uremic syndrome and infection by verotoxin-producing Escheri chia coli. J Infect Dis 1985; 151:775–782
    [Google Scholar]
  5. Taylor C. M., White R. H. R., Winterbom M. H., Rowe B. Haemolytic-uraemic syndrome: clinical experience of an outbreak in the West Midlands. BMJ 1986; 292:1513–1516
    [Google Scholar]
  6. Neill M. A., Tarr P. I., Clausen C. R., Christie D. L., Hickman R. O. Escherichia coli 0157:H7 as the predominant pathogen associated with hemolytic uremic syndrome: a pro spective study in the Pacific Northwest. Pediatrics 1987; 80:37–40
    [Google Scholar]
  7. Novillo A. A., Voyer L. E. Cravioto R et al. Hemolytic uremic syndrome associated with fecal cytotoxin and VT neutral izing antibodies. Ped Nephrol 1988; 2:288–290
    [Google Scholar]
  8. Chowdhry V. P., Bhuyan U. N., Arya L. S., Bhujwala R. A. Acuterenal failure following bacillary dysentery, possible mech anism of its pathogenesis. Indian J Pediatr 1983; 50:9–14
    [Google Scholar]
  9. Khoshoo V., Moudgil A., Vasudev A. S., Bhan M. K., Srivastava R. N. Haemolytic uraemic syndrome associated with salmon ella dysentery. Acta Paediatr Scand 1988; 77:604–605
    [Google Scholar]
  10. Malik S. K., Sirwal I. A., Pandit K. A., Kaul P. A., Najar M. S. Haemolytic uraemic syndrome—experience at Srinagar. Indian Pediatr 1990; 27:1098–1100
    [Google Scholar]
  11. Joshi W., Nagran B., Mehta S., Narang A., Mohanty D. Renal microvascular thrombosis with cortical necrosis in haemo lytic uraemic syndrome in association with acute infectious colitis. Indian Pediatr 1979; 16:749–754
    [Google Scholar]
  12. Dutta R., Bhattacharya S. K. Dutta D et al. Haemolytic uraemic syndrome following Salmonella typhimurium enteritis. Indian J Pediatr 1987; 54:743–745
    [Google Scholar]
  13. Chatterjee B. D., Sanyal S. N. Is this all shigellosis?. Lancet 1984; 2:574
    [Google Scholar]
  14. Chaturvedi P., Dey S. K., Aggarwal M. Haemolytic uraemic syndrome associated with Salmonella typhi. Indian Pediatr 1990; 27:209–210
    [Google Scholar]
  15. Nammalwar B. R., Balasubramanium N., Thingadurai C., Santhanakrishnan B. R. The haemolytic uraemic syndrome. Indian J Pediatr 1987; 54:746–748
    [Google Scholar]
  16. Ragupathy P., Date A., Sastry J. C. M., Sudarsanam A., Jadhav M. Haemolytic uraemic syndrome complicating shigella dysentery in South Indian children. BMJ 1978; 1:1518–1521
    [Google Scholar]
  17. Kishore K., Rattan A., Bagga A., Nath M., Srivastava R. N. Shriniwas. Failure to isolate verotoxin producing Escheri chia coli from patients with haemolytic uraemic syndrome. Indian J Pediatr (in press)
    [Google Scholar]
  18. Chart H., Scotland S. M., Rowe B. Serum antibodies to Escheri chia coli serotype 0157:H7 in patients with haemolytic uraemic syndrome. J Clin Microbiol 1989; 27:285–290
    [Google Scholar]
  19. Chart H., Scotland S. M., Smith H. R., Rowe B. Antibodies to Escherichia coli 0157 in patients with hemorrhagic colitis and haemolytic uraemic syndrome. J Clin Pathol 1989; 42:973–976
    [Google Scholar]
  20. Chart H., Smith H. R., Scotland S. M., Rowe B., Milford D. V., Taylor C. M. Serological identification of Escherichia coli 0157:H7 infection in haemolytic uraemic syndrome. Lancet 1991; 337:138–140
    [Google Scholar]
  21. Milford D. V., Taylor C. M. New insights into the haemolytic uraemic syndromes. Arch Dis Child 1990; 65:713–715
    [Google Scholar]
  22. Westphal O., Jann K. Bacterial lipopolysaccharide: extraction with phenol-water and further application of the procedure. Methods Carbohydr Chern 1965; 5:83–91
    [Google Scholar]
  23. Karmali M. A. Infection by verocytotoxin-producing Escheri chia coli. Clin Microbiol Rev 1989; 2:15–38
    [Google Scholar]
  24. Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. Verotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25:237–243
    [Google Scholar]
  25. Tarr P. I., Weill M. A., Clausen C. R., Watkins S. L., Christie D. L., Hickman R. O. Escherichia coli 0157:H57 and hemolytic uremic syndrome; importance of early cultures in est ablishing the etiology. J Infect Dis 1990; 162:553–556
    [Google Scholar]
  26. Krishnan C., Fitzgerald V. A., Dakin S. J., Behme R. J. Laboratory investigation of an outbreak of hemorrhagic colitis caused by Escherichia coli 0157:H7. J Clin Microbiol 1987; 25:1043–1047
    [Google Scholar]
  27. Notenboom R. H., Boreczyk A., Karmali M. A., Duncan L. M. C. Clinical relevance of a serological cross-reaction between Escherichia coli 0157 and Brucella abortus. Lancet 1987; 2:745
    [Google Scholar]
  28. Toth I., Barrett T. J., Cohen M. L., Rumschlag H. A. S., Green J. H., Wachsmuth I. K. Enzyme-linked immunosorbent assay for products of the 60-megadalton plasmid of Escherichia coli serotype 0157:H7. J Clin Microbiol 1991; 29:1016–1019
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-6-364
Loading
/content/journal/jmm/10.1099/00222615-37-6-364
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error