1887

Abstract

Summary

Two DNA fragments cloned from the genome of ATCC 10261 may be useful in the rapid diagnosis of disseminated candidosis. One sequence (probe EOB1) was specific for (positive hybridisation with 45 strains tested). The second sequence (probe EOB2) detected , as well as five other pathogenic spp. and , but did not react with human or bacterial DNA. Both probes were repetitive sequences in the genome of . Probe EOB1 was used to detect, without DNA amplification, 500 yeast cells in 1 ml of human blood.

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1992-11-01
2024-10-11
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References

  1. Musial C. E., Cockerill F. R., Roberts G. D. Fungal infections of the immunocompromised host: clinical and laboratory aspects. Clin Microbiol Rev 1988; 1:349–364
    [Google Scholar]
  2. Jones J. M. Laboratory diagnosis of invasive candidiasis. Clin Microbiol Rev 1990; 3:32–45
    [Google Scholar]
  3. Wey S. B., Mori M., Pfaller M. A., Woolson R. F., Wenzel R. P. Hospital-acquired candidemia. The attributable mortality and excess length of stay. Arch Intern Med 1988; 148:2642–2645
    [Google Scholar]
  4. Buchman T. G., Rossier M., Merz W. G., Charache P. Detection of surgical pathogens by in vitro DNA amplification. Part 1. Rapid identification of Candida albicans by in vitro amplification of a fungus-specific gene. Surgery 1990; 108:338–347
    [Google Scholar]
  5. Magee B. B., D’Souza T. M., Magee P. T. Strain and species identification by restriction fragment length polymor phisms in the ribosomal DNA repeat of Candida species. J Bacteriol 1987; 169:1639–1643
    [Google Scholar]
  6. Scherer S., Stevens D. A. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci USA 1988; 85:1452–1456
    [Google Scholar]
  7. Sadhu C., McEachern M. J., Rustchenko-Bulgac E. P., Schmid J., Soil D. R., Hicks J. B. Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification. J Bacteriol 1991; 173:842–850
    [Google Scholar]
  8. Cutler J. E., Glee P. M., Horn H. L. Candida albicans-and Candidastellatoidea-specific DNA fragment. J Clin Microbiol 1988; 26:1720–1724
    [Google Scholar]
  9. Soil D. R., Galask R., Isley S. Switching of Candida albicans during successive episodes of recurrent vaginitis. J Clin Microbiol 1989; 27:681–690
    [Google Scholar]
  10. Fox B. C., Mobley H. L. T., Wade J. C. The use of a DNA probe for epidemiological studies of candidiasis in immuno compromised hosts. J Infect Dis 1989; 159:488–494
    [Google Scholar]
  11. Cryer D. R., Eccleshall R., Marmur J. Isolation of yeast DNA. Methods Cell Biol 1975; 12:39–44
    [Google Scholar]
  12. Herrmann B. G., Frischauf A. -M. Isolation of genomic DNA. In Berger S. L., Kimmel A. R. (eds) Guide to molecular cloning techniques. Methods in enzymology vol 152 New York: Academic Press;180–182
    [Google Scholar]
  13. Jenkinson H. F., Schep G. P., Shepherd M. G. Cloning and expression of the 3-isopropylmalate dehydrogenase gene from Candida albicans. FEMS Microbiol Lett 1988; 49:285–288
    [Google Scholar]
  14. Cannon R. D., Jenkinson H. F., Shepherd M. G. Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae. Mol Gen Genet 1990; 221:210–218
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 1977; 113:237–251
    [Google Scholar]
  16. Dorn G. L., Smith K. New centrifugation blood culture device. Clin Microbiol 1978; 7:52–54
    [Google Scholar]
  17. Telenti A., Roberts G. D. Fungal blood cultures. Eur J Clin Microbiol Infect Dis 1989; 8:825–831
    [Google Scholar]
  18. Kiehn T. E. Bacteremia and fungemia in the immune compromised patient. Eur J Clin Microbiol Infect Dis 1989; 8:832–837
    [Google Scholar]
  19. Bryan C. S. Clinical implications of positive blood cultures. Clin Microbiol Rev 1989; 2:329–353
    [Google Scholar]
  20. Rubino S., Muresu R., Rappelli P. Molecular probe for identification of Trichomonas vaginalis DNA. J Clin Microbiol 1991; 29:702–706
    [Google Scholar]
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