1887

Abstract

Summary

A biphasic culture system, the MB Check, was compared with conventional culture on Löwenstein-Jensen egg (LJ) solid medium and with Bactec broth culture for primary isolation of mycobacteria from clinical samples. A total of 104 mycobacterial isolates was detected from 985 samples examined by the three methods. The most sensitive primary isolation was with LJ culture and MB Check; these methods detected 93% and 87% of all positive cultures, respectively. MB Check allowed a somewhat more rapid detection than LJ culture. The presence of atypical mycobacteria was demonstrated most rapidly with the Bactec system.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-5-332
1992-11-01
2023-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/5/medmicro-37-5-332.html?itemId=/content/journal/jmm/10.1099/00222615-37-5-332&mimeType=html&fmt=ahah

References

  1. Hoffner S. E. Improved detection of Mycobacterium avium complex with the Bactec radiometric system. Diagn Microbiol Infect Dis 1988; 10:1–6
    [Google Scholar]
  2. Hoffner S. E., Henriques B., Petrini B., Källenius G. Myco bacterium malmoense: an easily missed pathogen. J Clin Microbiol 1991; 29:2673–2674
    [Google Scholar]
  3. Martin T., Cheke D., Natyshak I. Broth culture: the modem “guinea-pig” for isolation of mycobacteria. Tubercle 1989; 70:53–56
    [Google Scholar]
  4. Groothuis D. G., Yates M. D. (eds). Manual of diagnostic and public health mycobacteriology. 2nd edn London: European Society for Mycobacteriology; 1991
    [Google Scholar]
  5. Middlebrook G., Reggiardo Z., Tigertt W. D. Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media. Am Rev Respir Dis 1977; 115:1066–1069
    [Google Scholar]
  6. Giger T., Burkardt H. J. Evaluation of a new biphasic culture system for the recovery of mycobacteria. Eur J Clin Microbiol Infect Dis 1990; 9:428–431
    [Google Scholar]
  7. Kent P. T., Kubica G. P. (eds). Public health mycobacteriology, a guide for the level III laboratory. Atlanta: Centers for Disease Control; 1985
    [Google Scholar]
  8. Fadda G., Roe S. L. Recovery and susceptibility testing of Mycobacterium tuberculosis from extrapulmonary specimens by the Bactec radiometric method. J Clin Microbiol 1984; 19:720–721
    [Google Scholar]
  9. Damato J. J., Collins M. T., Rothlauf M. V., McClatchy J. K. Detection of mycobacteria by radiometric and standard plate procedures. J Clin Microbiol 1983; 17:1066–1073
    [Google Scholar]
  10. Salfinger M., Demchuk B. S., Kafader F. M. Comparison between MB check broth radiometric and conventional methods for recovery of mycobacteria from respiratory specimens. J Microbiol Methods 1990; 12:97–100
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-5-332
Loading
/content/journal/jmm/10.1099/00222615-37-5-332
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error