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Volume 37, Issue 5

Differentiated Caco-2 cells as a model for enteric invasion by and Free

Abstract

Summary

A collection of 44 isolates (37 and seven ) from children with colitis (21 strains) or watery diarrhoea (23 strains) was analysed for toxin production, association with HeLa cells, and invasion of differentiated Caco-2 cell cultures. There was no obvious association of clinical symptoms with species, biotype or enterotoxin production. All colitis strains and most of the isolates from watery diarrhoea were cytotoxic for Chinese hamster ovary cells. Measurements of bacterial association indices with HeLa cells varied with time, and were considered to be unreliable for discriminating between isolates from the two diagnostic groups. Statistically significant differences were observed between the two groups (all colitis strains and 65% of strains from non-inflammatory diarrhoea) with respect to invasion of both HeLa and Caco-2 cell monolayers. However, among the strains from non-inflammatory diarrhoea that did invade, numbers of internalised bacteria were similar to the range observed for colitis strains. Of the colitis strains, 86% were able to transcytose through polarised Caco-2 monolayers grown on filters, compared with 48% of isolates from non-inflammatory disease. We propose the use of Caco-2 cells as a model for studying invasion of intestinal epithelia by and

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© J. MED. MICROBIOL. 1992
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1992-11-01
2024-04-20
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References

  1. Blaser M. J., Wells J. G., Feldman R. A., Pollard R. A., Allen J. R. and the Collaborative Diarrheal Disease Study Group. Campylobacter enteritis in the United States. A multicenter study. Ann Intern Med 1983; 98:360–365
     [Google Scholar]
  2. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol 1979; 8:737–765
     [Google Scholar]
  3. Mandal B. K., De Mol P., Butzler J. -P. Clinical aspects of Campylobacter infections in humans. In Butzler J.-P. (ed) Campylobacter infection in man and animals Boca Raton: FA. CRC Press Inc; 198421–32
     [Google Scholar]
  4. Blaser M. J., Perez G. P., Smith P. F. Extraintestinal Campylobacter jejuni and Campylobacter coli infections: host factors and strain characteristics. J Infect Dis 1986; 153:552–559
     [Google Scholar]
  5. Fox G. J. Campylobacteriosis—a “new” disease in laboratory animals. Lab Animal Sci 1982; 32:625–637
     [Google Scholar]
  6. Klipstein F. A., Engert R. F., Short H., Schenk E. A. Pathogenic properties of Campylobacter jejuni: assay and correlation with clinical manifestations. Infect Immun 1985; 50:43–49
     [Google Scholar]
  7. Van Spreeuwell J. P., Duursma G. C., Meijer C. J. L. M., Bax R., Rosekrans P. C. M., Lindeman J. Campylobacter colitis: histological immunohistochemical and ultrastructural findings. Gut 1985; 26:945–951
     [Google Scholar]
  8. Sérény B. Experimental keratoconjunctivitis Shigellosa. Acta Microbiol Hung 1957; 4:367–376
     [Google Scholar]
  9. Guerrant R. L., Lahita R. G., Winn W. C., Roberts R. B. Campylo bacteriosis in man: pathogenic mechanisms and review of 91 bloodstream infections. Am J Med 1978; 65:584–592
     [Google Scholar]
  10. Belbouri A., Mégraud F. Enterotoxin-like activity produced by Campylobacter jejuni and Campylobacter coli isolated from patients and healthy controls in Algeria. FEMS Microbiol Lett 1988; 51:25–28
     [Google Scholar]
  11. Johnson W. M., Lior H. Toxins produced by Campylobacter jejuni and Campylobacter coli. Lancet 1984; 1:229–230
     [Google Scholar]
  12. Klipstein F. A., Engert R. F. Immunological relationship of the B subunits of Campylobacter jejuni and Escherichia coli heat-labile enterotoxins. Infect Immun 1985; 48:629–633
     [Google Scholar]
  13. Ruiz-Palacios G. M., Torres J., Torres N. I., Escamilla E., Ruiz-Palacios B. R., Tamayo J. Cholera-like enterotoxin produced by Campylobacter jejuni. Characterisation and clinical significance. Lancet 1983; 2:250–253
     [Google Scholar]
  14. Fauchere J. L., Rosenau A., Veron M., Moyen E. N., Richard S., Pfister A. Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. Infect Immun 1986; 54:283–287
     [Google Scholar]
  15. Manninen K. I., Prescott J. F., Dohoo I. R. Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infect Immun 1982; 38:46–52
     [Google Scholar]
  16. Newell D. G., McBride H., Saunders F., Dehele Y., Pearson A. D. The virulence of clinical and environmental isolates of Campylobacter jejuni. J Hyg Camb 1985; 94:45–54
     [Google Scholar]
  17. Newell D. G., Pearson A. The invasion of epithelial cell lines and the intestinal epithelium of infant mice by Campylobacter jejuni/coli. J Diarrhoeal Dis Res 1984; 2:19–26
     [Google Scholar]
  18. Pinto M., Robine-Leon S., Appay M. -D. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell 1983; 47:323–330
     [Google Scholar]
  19. Rousset M. The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochimie 1986; 68:1035–1040
     [Google Scholar]
  20. Lior H. New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and “Campylobacter laridis”. J Clin Microbiol 1984; 20:636–640
     [Google Scholar]
  21. Goossens H., Butzler J.-P., Takeda Y. Demonstration of cholera like enterotoxin production by Campylobacter jejuni. FEMS Microbiol Lett 1985; 29:73–76
     [Google Scholar]
  22. Finlay B. B., Gumbiner B., Falkow S. Penetration of Salmonella through a polarized Madin-Darby canine kidney epithelial cell monolayer. J Cell Biol 1988; 107:221–230
     [Google Scholar]
  23. Klipstein F. A., Engert R. F., Short H. B. Enzyme-finked immune sorbent assays for virulence properties of Campylobacter jejuni clinical isolates. J Clin Microbiol 1986; 23:1039–1043
     [Google Scholar]
  24. Lindblom G. B., Johny M., Khalil K., Mazhar K., Ruiz-Palacios G. M., Kaijser B. Enterotoxigenicity and frequency of Campylobacter jejuni, C. coli and C. laridis in human and animal stool isolates from different countries. FEMS Microbiol Lett 1990; 54:163–167
     [Google Scholar]
  25. Mathan V. I., Rajan D. P., Klipstein F. A., Engert R. F. Entero toxigenic Campylobacter jejuni among children in South India. Lancet 1984; 2:981
     [Google Scholar]
  26. Ruiz-Palacios G. M., Lopez-Vidal Y., Torres J., Torres N. Serum antibodies to heat-labile enterotoxin of Campylobacter jejuni. J Infect Dis 1985; 152:413–416
     [Google Scholar]
  27. Yeen W. P., Puthucheary S. D., Pang T. Demonstration of a cytotoxin from Campylobacter jejuni. J Clin Pathol 1983; 36:1237–1240
     [Google Scholar]
  28. Perez-Perez G. I., Cohn D. L., Guerrant R. L., Patton C. M., Reller L. B., Blaser M. J. Clinical and immunologic significance of cholera-like toxin and cytotoxin production by Campylobacter species in patients with acute inflammatory diarrhea in the USA. J Infect Dis 1989; 160:460–468
     [Google Scholar]
  29. Grasset E., Bernabeu J., Pinto M. Epithelial properties of human colonic carcinoma cell line Caco-2: effect of secretagogues. Am J Physiol 1985; 248:C410–C418
     [Google Scholar]
  30. Grasset E., Pinto M., Dussaulx E., Zweibaum A., Desjeux J. -F. Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters. Am J Physiol 1984; 247:C260–C267
     [Google Scholar]
  31. Mostov K. E., Simister N. E. Transcytosis. Cell 1985; 43:389–390
     [Google Scholar]
  32. De Melo M. A., Pechere J. -C. Identification of Campylobacter jejuni surface proteins that bind to eukaryotic cells in vitro. Infect Immun 1990; 58:1749–1756
     [Google Scholar]
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Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli
J. Med. Microbiol. 37, 319 (1992); https://doi.org/10.1099/00222615-37-5-319
/content/journal/jmm/10.1099/00222615-37-5-319
/content/journal/jmm/10.1099/00222615-37-5-319
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