1887

Abstract

Summary

The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of H-leucine-labelled after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes were injected into experimental animals. Blood and tissue samples were obtained up to 24 h after injection and processed for liquid scintillation counting (to determine the distribution of labelled yeasts) and quantitation of viable organisms. Yeasts were cleared rapidly after intravenous (i.v.) injection by either route, i.e., < 5% of the radioactivity was detected in the blood after 5 min. The liver and lung were the major organs that sequestered blood-borne yeasts 1 h after tail vein injection (42.5 SD 15% and 41.4 SD 6.4% of labelled yeasts injected, respectively). However, injections the portal vein resulted in trapping of the yeasts predominantly by the liver. Recovery of radioactivity and viable yeasts from all organs except the kidneys decreased with time. Overall, the results indicated that the rat might serve as a reliable model for short-term studies on organ distribution and thus contribute to our understanding of tissue trophism in candidosis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-4-291
1992-10-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/4/medmicro-37-4-291.html?itemId=/content/journal/jmm/10.1099/00222615-37-4-291&mimeType=html&fmt=ahah

References

  1. Myerowitz R. L., Pazin G. J., Allen C. M. Disseminated candidiasis: changes in incidence, underlying diseases, and pathology. Am J Clin Pathol 1977; 68:29–38
    [Google Scholar]
  2. Solomkin J. S., Flohr A. M., Simmons R. L. Indications for therapy for fungemia in postoperative patients. Arch Surg 1982; 117:1272–1275
    [Google Scholar]
  3. Butler K. M., Baker C. J. Candida: an increasingly important pathogen in the nursery. PedClin N Am 1988; 35:543–563
    [Google Scholar]
  4. Stone H. H., Kolb L. D., Currie C. A., Geheber C. E., Cuzzell J. Z. Candida sepsis: pathogenesis and principles of treatment. Ann Surg 1974; 179:697–711
    [Google Scholar]
  5. Stone H. H. Studies in the pathogenesis, diagnosis, and treatment of Candida sepsis in children. J Ped Surg 1974; 9:127–133
    [Google Scholar]
  6. Baine W. B., Koenig M. G., Goodman J. S. Clearance of Candida albicans from the bloodstream of rabbits. Infect Immun 1974; 10:1420–1425
    [Google Scholar]
  7. Evans Z. A., Mardon D. N. Organ localization in mice challenged with a typical Candida albicans strain and a pseudohyphal variant (39780). Proc Soc Exp Biol Med 1977; 155:234–238
    [Google Scholar]
  8. Trnovec T., Sikl D., Zemanek M. The distribution in mice of intravenously administered labelled Candida albicans . Sabouraudio 1978; 16:299–306
    [Google Scholar]
  9. Louria D. B., Brayton R. G., Finkel G. Studies on the pathogenesis of experimental Candida albicans infections in mice. Sabouraudia 1963; 2:271–283
    [Google Scholar]
  10. Iannini P. B., Arai G. D., LaForce F. M. Vascular clearance of blastospore and pseudomycelial phase Candida albicans . Sabouraudia 1977; 15:201–205
    [Google Scholar]
  11. Gelfand J. A., Hurley D. L., Fauci A. S., Frank M. M. Role of complement in host defense against experimental disseminated candidiasis. J Infect Dis 1978; 138:9–16
    [Google Scholar]
  12. Allen C. M., Beck F. M. Strain-related differences in patho genicity of Candida albicans for oral mucosa. J Infect Dis 1983; 147:1036–1040
    [Google Scholar]
  13. Howlett J. A. The infection of rat tongue mucosa in vitro with five species of Candida . J Med Microbiol 1976; 9:309–316
    [Google Scholar]
  14. Sobel J. D., Muller G., Buckley H. R. Critical role of germ tube formation in the pathogenesis of candidal vaginitis. Infect Immun 1984; 44:576–580
    [Google Scholar]
  15. Kinsman O. S., Collard A. E. Hormonal factors in vaginal can didiasis in rats. Infect Immun 1986; 53:498–504
    [Google Scholar]
  16. Tarry W. F., Kowalkowski T. S., Fisher M. A. Renal candidiasis in the rat: effects of ureteral obstruction and diabetes. Urol Res 1989; 17:367–370
    [Google Scholar]
  17. Levison M. E., Pitsakis P. G. Susceptibility to experimental Can dida albicans urinary tract infection in the rat. J Infect Dis 1987; 155:841–846
    [Google Scholar]
  18. DeMaria A., Buckley H., Von Lichtenberg F. Gastrointestinal candidiasis in rats treated with antibiotics, cortisone, and azathioprine. Infect Immun 1976; 13:1761–1770
    [Google Scholar]
  19. Katz S., Merkel G. J., Folkening W. J., Rosenthal R. S., Grosfeld J. L. Impaired clearance and organ localization of Candida albicans in obstructive jaundice. J Ped Surg 1991; 26:904–907
    [Google Scholar]
  20. Merkel G. J., Phelps C. L. Factors influencing the interaction of Candida albicans with fibroblast cell cultures. Infect Immun 1988; 56:792–801
    [Google Scholar]
  21. Merkel G. J., Phelps C. L. Conditions affecting the amphotericin B mediated inhibition of Candida albicans attachment to cell cultures. Can J Microbiol 1989; 35:260–264
    [Google Scholar]
  22. Sawyer R. T. Experimental pulmonary candidiasis. Mycopa-thologia 1990; 109:99–109
    [Google Scholar]
  23. Jeunet F. S., Meuwissen H. J., Good R. A. Fate of Candida albicans in neonatally thymectomized rats. Proc Soc Exp Biol Med 1970; 133:53–56
    [Google Scholar]
  24. Odds F. C. Candida and candidosis. 2nd edn London: Bailliere Tindall; 1988252–278
    [Google Scholar]
  25. Ruchel R., Uhlemann K., Boning B. Secretion of acid proteinases by different species of the genus Candida . Zentralbl Bakteriol Mikrobiol Hyg A 1983; 255:537–548
    [Google Scholar]
  26. MacDonald F. Secretion of inducible proteinase by pathogenic Candida species. Sabouraudia 1984; 22:79–82
    [Google Scholar]
  27. Barrett-Bee K., Hayes Y., Wilson R. G., Ryley J. F. A comparison of phospholipase activity, cellular adherence and pathogenicity of yeasts. J Gen Microbiol 1985; 131:1217–1221
    [Google Scholar]
  28. Cutler J. E., Friedman L., Milner K. C. Biological and chemical characterization of toxic substances from Candida albicans . Infect Immun 1972; 6:616–627
    [Google Scholar]
  29. Blyth W., Stewart G. E. Systemic candidiasis in mice treated with prednisolone and amphotericin B. 2. Ultrastructure and evidence for fungal toxin. Mycopathologia 1978; 66:51–57
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-4-291
Loading
/content/journal/jmm/10.1099/00222615-37-4-291
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error