1887

Abstract

Summary

A collection of 86 strains of of serotype Typhimurium isolated from children with gastroenteritis in Tehran, Iran was examined for biochemical phenotype, phage type and antibiotic-resistance pattern. Twenty-seven biochemical phenotypes (BPTs), 14 discrete phage types (PTs) and 18 resistotypes (RTs) were identified. Fifty-three strains (62%) belonged to two major and probably related BPTs, whereas the other 33 isolates belonged to less common BPTs. The two predominant BPTs contained 26 strains of the same PT and 23 strains of the same RT. Different PTs and RTs of strains with similar BPT were sometimes observed, possibly reflecting antibiotic pressures in Iran. These results suggest that two major “clones” of Typhimurium strains are particularly common in Iran and, although each method alone adequately detected these and other less common “clones”, biochemical fingerprinting provided additional information about relationships among strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-4-252
1992-10-01
2023-02-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/4/medmicro-37-4-252.html?itemId=/content/journal/jmm/10.1099/00222615-37-4-252&mimeType=html&fmt=ahah

References

  1. Mitchell E., O’Mahony M., Lynch D. Large outbreak of food poisoning caused by Salmonella typhimurium definitive type 49 in mayonnaise. BMJ 1989; 298:99–101
    [Google Scholar]
  2. Fule R. P., Ninawe R. W., Saoji A. M. Outbreak of gastroenteritis due to Salmonella typhimurium in Solapur. Indian Pediatr 1988; 25:57–60
    [Google Scholar]
  3. Turnbull P. C. B. Food poisoning with special reference to Salmonella–its epidemiology, pathogenesis and control. Clin Gastroenterol 1979; 8:663–714
    [Google Scholar]
  4. Farhoudi-Moghaddam A. A., Katouli M., Jafari A., Behavar M. A., Parsi M., Malekzadah F. Antimicrobial drug resistance transfer factor among clinical isolates of salmonellae in Iran. Scan J Infect Dis 1990; 22:197–203
    [Google Scholar]
  5. Duguid J. P., Anderson E. S., Alfredsson G. A., Barker R., Old D. C. A new biotyping scheme for Salmonella typhimurium and its phylogenetic significance. J Med Microbiol 1975; 8:149–166
    [Google Scholar]
  6. Anderson E. S., Ward L. R., De Saxe M. J., De Sa J. D. H. Bacterio phage-typing designations of Salmonella typhimurium . J Hyg 1977; 78:297–300
    [Google Scholar]
  7. Guinee P. A. M., Jansen W. H., Van Schuylenberg A., Van Leeuwen J. W. Bacteriophage typing of Salmonella typhimurium by use of a mechanized technique. Appl Microbiol 1973; 26:474–477
    [Google Scholar]
  8. Anderson E. S. Drug resistance in Salmonella typhimurium and its implications. BMJ 1968; 3:333–339
    [Google Scholar]
  9. Brunner F., Margadant A., Peduzzi R., Piffaretti J.-C. The plasmid pattern as an epidemiologic tool for Salmonella typhimurium epidemics: comparison with the lysotype. J Infect Dis 1983; 148:7–11
    [Google Scholar]
  10. Taylor D. N., Wachsmuth I. K., Shangkuan Y.-H. Salmon ellosis associated with marijuana. A multistate outbreak traced by plasmid fingerprinting. N Engl J Med 1982; 306:1249–1253
    [Google Scholar]
  11. Kapperud G., Lassen J., Dommarsnes K. Comparison of epidemiological marker methods for identification of Salmonella typhimurium isolates from an outbreak caused by contaminated chocolate. J Clin Microbiol 1989; 27:2019–2024
    [Google Scholar]
  12. Platt D. J., Brown D. J., Old D. C., Barker R. M., Munro D. S., Taylor J. Old and new techniques together resolve a problem of infection by Salmonella typhimurium . Epidemiol Infect 1987; 99:137–142
    [Google Scholar]
  13. Threlfall E. J., Frost J. A. The identification, typing and finger printing of Salmonella: laboratory aspects and epidemiological applications. J Appl Bacteriol 1990; 68:5–16
    [Google Scholar]
  14. Barker R. M., Old D. C. The usefulness of biotyping in studying the epidemiology and phylogeny of salmonellae. J Med Microbiol 1989; 29:81–88
    [Google Scholar]
  15. Kuhn I. Biochemical fingerprinting of Escherichia coli: a simple method for epidemiological investigations. J Microbiol Methods 1985; 3:159–170
    [Google Scholar]
  16. Kuhn I., Burman L. G., Eriksson L., Mollby R. Subtyping of Klebsiella by biochemical fingerprinting: a simple system for epidemiological investigations. J Microbiol Methods 1990; 11:177–185
    [Google Scholar]
  17. Katouli M., Kuhn I., Wollin R., Mollby R. Evolution of the PhP system for biochemical-fingerprint typing of strains of Salmonella of serotype Typhimurium. J Med Microbiol 1992; 37:245–251
    [Google Scholar]
  18. Lilleengen K. Typing of Salmonella typhimurium by means of bacteriophage. Acta Pathol Microbiol Scand Suppl LXXVII: 1948
    [Google Scholar]
  19. Sneath P. H. A., Sokal R. R. Numerical taxonomy. The principles and practice of numerical classification. San Francisco: W. H. Freeman; 1973
    [Google Scholar]
  20. Eisenstein B. I. New molecular techniques for microbial epi demiology and the diagnosis of infectious diseases. J Infect Dis 1990; 161:595–602
    [Google Scholar]
  21. Barker R. M., Old D. C., Munro D. S., Taylor J. Prevalent phage types of Salmonella typhimurium in Scotland. Communicable Diseases Scotland Weekly Report 1987; 20:5–7
    [Google Scholar]
  22. Barker R. M., Old D. C. Biotypes of strains of Salmonella typhimurium of phage types 49, 204 and 193. J Med Microbiol 1980; 13:369–371
    [Google Scholar]
  23. Anderson E. S. Influence of the A transfer factor on the phage sensitivity of salmonellae. Nature 1966; 212:795–799
    [Google Scholar]
  24. Anderson E. S., Threlfall E. J., Carr J. M., Savoy L. G. Bacteriophage restriction in Salmonella typhimurium by R factors and transfer factors. J Hyg 1973; 71:619–631
    [Google Scholar]
  25. Katouli M., Pachenary A., Jafari A. The role of Shigella spp. in childhood diarrhoea in Iran and their antibiotic resistance. Scand J Infect Dis 1989; 21:415–419
    [Google Scholar]
  26. Barker R. M., Old D. C., Sharp J. C. M. Phage type/biotype groups of Salmonella typhimurium in Scotland 1974–6: variation during spread of epidemic clones. J Hyg 1980; 84:115–125
    [Google Scholar]
  27. Anderson E. S. The modern ecological study of Salmonella typhimurium infection. In Peleaz Perez-Miravete A. (eds) Xth International Congress for Microbiology, Mexico City 1970 (Proceedings). Recent advances in microbiology 1971381
    [Google Scholar]
  28. Katouli M., Kuhn I., Mollby R. Evaluation of the stability of biochemical phenotypes of Escherichia coli upon subculturing and storage. J Gen Microbiol 1990; 136:1681–1688
    [Google Scholar]
  29. Morgenroth A., Duguid J. P. Demonstration of different muta tional sites controlling rhamnose fermentation in FIRN and non-FIRN rha-strains of Salmonella typhimurium: an essay in bacterial archaeology. Gen Res 1968; 11:151–169
    [Google Scholar]
  30. Anderson E. S., Ward De Saxe M. J., Old D. C., Barker R., Duguid J. P. Correlation of phage type, biotype and source in strains of Salmonella typhimurium . J Hyg 1978; 81:203–217
    [Google Scholar]
  31. Old D. C. Temperature-dependent utilization of meso-inositol: a useful biotyping marker in the genealogy of Salmonella typhimurium . J Bacteriol 1972; 112:779–783
    [Google Scholar]
  32. Old D. C., Duguid J. P. Transduction of fimbriation demonstrating common ancestry in FIRN strains of Salmonella typhimurium . J Gen Microbiol 1979; 112:251–259
    [Google Scholar]
  33. Old D. C. Phylogeny of strains of Salmonella typhimurium . Microbiol Sciences 1984; 1:69–72
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-4-252
Loading
/content/journal/jmm/10.1099/00222615-37-4-252
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error