1887

Abstract

Summary

The treatment of abscess homogenates with calcium ionophores stimulated the production of a bactericidal lipid with properties indistinguishable from those of a previously unidentified bactericidal lipid that had been detected in staphylococcal abscesses. The lipid was identified as a monoglyceride by thin layer chromatography. It resembled the unidentified lipid in that it had a high specific activity, exhibited differential activity, was inhibited by δ toxin, lecithin and Ca, and its activity was reduced by oxidation. Stimulation of monoglyceride production by calcium ionophore requires the joint presence of components from the sedimented and supernatant fractions of abscess homogenates, and was not produced if boiled homogenate was used. The addition of verapamil interfered with the production of monoglyceride in homogenates treated with calcium ionophore. Monoglyceride was produced only in abscess homogenates and not in homogenates of other normal tissues or tissues taken from mice infected with . Calcium ionophore could be replaced by inositol triphosphate, suggesting that monoglyceride production involved the release of calcium from intracellular stores. The 2-monoglyceride was the form originally produced in abscess homogenates, but this spontaneously isomerised to the 1-monoglyceride. The fatty-acid moiety of the monoglyceride consisted primarily of 16:0 and 16:1 fatty acids.

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1992-10-01
2022-01-24
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References

  1. Dye E. S., Kapral F. A. Survival of Staphylococcus aureus in intraperitoneal abscesses. J Med Microbiol 1981; 14:185–194
    [Google Scholar]
  2. Dye E. S., Kapral F. A. Characterization of a bactericidal lipid developing within staphylococcal abscesses. Infect Immun 1981; 32:98–104
    [Google Scholar]
  3. Dye E. S., Kapral F. A. Partial characterization of a bactericidal system in staphylococcal abscesses. Infect Immun 1980; 30:198–203
    [Google Scholar]
  4. Kapral F. A., Mortensen J. E. Mechanisms of resistance to staphylocidal fatty acids in abscesses. In Jeljaszewicz J. (ed) The staphylococci Zentralbl Bakteriol Suppl 14 Stuttgart: Gustav Fischer Verlag; 1985507–509
    [Google Scholar]
  5. Mortensen J. E., Shryock T. R., Kapral F. A. Modification of bactericidal fatty acids by an enzyme of Staphylococcus aureus . J Med Microbiol 1992; 36:293–298
    [Google Scholar]
  6. Kapral F. A., Godwin J. R., Dye E. S. Formation of intraperitoneal abscesses by Staphylococcus aureus. Infect Immun 1980; 30:204–211
    [Google Scholar]
  7. Dole V. P., Meinertz H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem 1960; 235:2595–2599
    [Google Scholar]
  8. Chemick S. S. Determination of glycerol in acyl glycerol. In Lowenstein J. M. (ed) Methods in enzymology vol XIV Lipids New York: Academic Press; 1969627–630
    [Google Scholar]
  9. Lambert M., Neish A. C. Rapid method for estimation of a glycerol in fermentation solutions. Cand J Res, Sect B 1950; 28:83–89
    [Google Scholar]
  10. Hanahan D. J., Olley J. N. Chemical nature of monophospho-inositides. J Biol Chem 1958; 231:813–828
    [Google Scholar]
  11. Kapral F. A., Smith S., Lai D. The esterification of fatty acids by Staphylococcus aureus fatty modifying enzyme (FAME) and its inhibition by glycerides. J Med Microbiol 1992; 37:235–237
    [Google Scholar]
  12. Nolte F. S., Kapral F. A. Hydrophobic interaction chromate graphy of Staphylococcus aureus delta-toxin. Infect Immun 1981; 31:1094–1098
    [Google Scholar]
  13. Vaskovsky V. E., Kostetsky E. Y. Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 1968; 9:396
    [Google Scholar]
  14. Svennerholm L. The quantitative estimation of cerebrosides in nervous tissue. J Neurochem 1956; 1:42–53
    [Google Scholar]
  15. Skipski V. P., Peterson R. F., Barclay M. Separation of phospha tidyl ethanolamine, phosphatidyl serine, and other phospholipids by thin-layer chromatography. J Lipid Res 1962; 3:467–470
    [Google Scholar]
  16. Christie W. W. Lipid analysis. 2nd edn Oxford: Pergamon Press; 1982120
    [Google Scholar]
  17. Svennerholm L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta 1957; 24:604–611
    [Google Scholar]
  18. Bischel M. D., Austin J. H. A modified benzidine method for the chromatographic detection of sphingolipids and acid polysaccharides. Biochim Biophys Acta 1963; 70:598–600
    [Google Scholar]
  19. Yamamoto A., Rouser G. Spectrophotometric determination of molar amounts of glycosphingolipids and ceramide by hydrolysis and reaction with trinitrobenzenesulfonic acid. Lipids 1970; 5:442–444
    [Google Scholar]
  20. Berridge M. J. The molecular basis of communication within the cell. Sci Am 1985; 253 (4):124–134
    [Google Scholar]
  21. Pollack J. D., Clark D. S., Somerson N. L. Four-directional-development thin-layer chromatography of lipids using trimethyl borate. J Lipid Res 1971; 12:563–569
    [Google Scholar]
  22. Fales H. M., Jaouni T. M., Babashak J. F. Simple device for preparing ethereal diazomethane without resorting to codistillation. Anal Chem 1973; 45:2302–2303
    [Google Scholar]
  23. Mattson F. H., Volpenhein R. A. Synthesis and properties of glycerides. J Lipid Res 1962; 3:281–296
    [Google Scholar]
  24. Berridge M. J. Inositol triphosphate and diacylglycerol as second messengers. Biochem J 1984; 220:345–360
    [Google Scholar]
  25. Berridge M. J., Irvine R. F. Inositol triphosphate, a novel second messenger in cellular signal transduction. Nature 1984; 312:315–321
    [Google Scholar]
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