Extracellular proteins as a potential marker of active infection in bone Free

Abstract

Summary

The potential of extracellular protein antigens of as markers of infection of bone was investigated by immunoblotting. Serum from patients with bone infection showed levels of IgG antibodies to a preparation of soluble extracellular proteins of 17–81 kDa which were significantly higher than those found in serum from normal controls. By contrast, immunoblots of various whole cell or cell wall-derived antigens gave complex patterns of response which were unsuitable for positive diagnosis of bone infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-4-227
1992-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/4/medmicro-37-4-227.html?itemId=/content/journal/jmm/10.1099/00222615-37-4-227&mimeType=html&fmt=ahah

References

  1. Wergeland H. I., Haaheim L. R., Natas O. B., Wesenberg F., Oeding P. Antibodies to staphylococcal peptidoglycan and its peptide epitopes, teichoic acid, and lipoteichoic acid in sera from blood donors and patients with staphylococcal infections. J Clin Microbiol 1989; 27:1286–1291
    [Google Scholar]
  2. Cheung A. L., Bayer A. S., Peter J., Ward J. I. Surface proteins of Staphylococcus aureus . Rev Infect Dis 1988; 10: Suppl 2S351–S355
    [Google Scholar]
  3. Thomas V. L., Sanford B. A., Keogh B. S., Triplett R. G. Antibody response to Staphylococcus aureus surface proteins in rabbits with persistent osteomyelitis after treatment with demineralized bone implants. Infect Immun 1989; 57:404–412
    [Google Scholar]
  4. Power M. E., Olson M. E., Domingue P. A. G., Costerton J. W. A rat model of Staphylococcus aureus chronic osteomyelitis that provides a suitable system for studying human infection. J Med Microbiol 1990; 33:189–198
    [Google Scholar]
  5. Arvidson S. O. Extracellular enzymes from Staphylococcusaureus . In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections London: Academic Press; 1983; 2745–808
    [Google Scholar]
  6. Krikler S. J., Pennington T. H., Petrie D. Typing of strains of Staphylococcus aureus by Western blot analysis of culture supemates. J Med Microbiol 1986; 21:169–171
    [Google Scholar]
  7. Lugtenberg B., Meijers J., Peters R., Van Der Hoek P., Van Alphen L. Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands. FEBS Lett 1975; 58:254–258
    [Google Scholar]
  8. Espersen F., Schiotz P. O. Normally–occurring precipitating antibody against Staphylococcus aureus in human serum and colostrum. Acta Pathol Microbiol Scand Sect C 1981; 89:93–98
    [Google Scholar]
  9. Bell J. A., Pennington T. H., Petrie D. T. Western blot analysis of staphylococcal antibodies present in human sera during health and disease. J Med Microbiol 1987; 23:95–99
    [Google Scholar]
  10. Jeljaszewicz J., Switalski L. M., Adlam C. Staphylocoagulase and clumping factor. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections London: Academic Press; 1983; 2525–527
    [Google Scholar]
  11. Bjӧrk I., Petersson B. A., Sjӧquist J. Some physiochemical properties of protein A from Staphylococcus aureus . Eur J Biochem 1972; 29:579–584
    [Google Scholar]
  12. Arbuthnott J. P. Epidermolytic toxins. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections London: Academic Press; 1983; 2599–617
    [Google Scholar]
  13. Schlievert P. M., Schoettle D. J., Watson D. W. Purification and physicochemical and biological characterisation of a staphylococcal pyrogenic exotoxin. Infect Immun 1979; 23:609–617
    [Google Scholar]
  14. Schlievert P. M. Purification and characterization of staphy-lococcal pyrogenic exotoxin type B. Biochemistry 1980; 19:6204–6208
    [Google Scholar]
  15. Schlievert P. M., Shands K. N., Dan B. B., Schmid G. P., Nishimura R. D. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome. J Infect Dis 1981; 143:509–516
    [Google Scholar]
  16. Igarashi H., Fijikawa H., Usami H., Kawabata S., Morita T. Purification and characterization of Staphylococcus aureus FRI 1169 and 587 toxic shock syndrome exotoxins. Infect Immun 1984; 44:175–181
    [Google Scholar]
  17. Bergdoll M. S. Enterotoxins. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections London: Academic Press; 1983; 2559–598
    [Google Scholar]
  18. Bergdoll M. S., Crass B. A., Reiser R. F., Robbins R. N., Davis J. P. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic–shock–syndrome Staphylococcus aureus isolates. Lancet 1981; 1:1017–1021
    [Google Scholar]
  19. Mollby R. Isolation and properties of membrane damaging toxins. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections London: Academic Press; 1983; 2619–669
    [Google Scholar]
  20. Mayberry-Carson K. J., Tober-Meyer B., Smith J. K., Lambe D. W., Costerton J. W. Bacterial adherence and glycocalyx formation in osteomyelitis experimentally induced with Staphylococcus aureus . Infect Immun 1984; 43:825–833
    [Google Scholar]
  21. HØiby N., Flensborg E. W., Beck B., Friis B., Jacobsen S. V., Jacobsen L. Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins by means of crossed immune-electrophoresis. Scand J Respir Dis 1977; 58:65–79
    [Google Scholar]
  22. Shorrock P. J., Lambert P. A., Aitchison E. J., Smith E. G., Farrell I. D., Gutschik E. Serological response in Enterococcus faecalis endocarditis determined by enzyme-linked immunosorbent assay. J Clin Microbiol 1990; 28:195–200
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-4-227
Loading
/content/journal/jmm/10.1099/00222615-37-4-227
Loading

Data & Media loading...

Most cited Most Cited RSS feed