%0 Journal Article %A Thomas, W. %A Sellwood, R. %T Monoclonal antibodies to a 16-kDa antigen of Serpulina (Treponema) hyodysenteriae %D 1992 %J Journal of Medical Microbiology, %V 37 %N 3 %P 214-220 %@ 1473-5644 %R https://doi.org/10.1099/00222615-37-3-214 %I Microbiology Society, %X Summary Monoclonal antibodies (MAbs) were produced to an outer-envelope preparation from Serpulina (Treponema) hyodysenteriae, the aetiological agent of swine dysentery. Three MAbs (isotype IgG1) were obtained. All three recognised a 16-kDa antigen that was common to most strains of S. hyodysenteriae of different serotypes but was absent from non-pathogenic, porcine intestinal spirochaetes. Immunofluorescence and immunogold labelling studies showed that the 16-kDa antigen was exposed on the surface of intact spirochaetes. The MAbs agglutinated freshly grown cultures of spirochaetes and inhibited growth of S. hyodysenteriae strains in vitro. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-37-3-214