1887

Journal of Medical Microbiology logo

Volume 37, Issue 3

Comparison of a blood-free medium and a filtration technique for the isolation of spp. from diarrhoeal stools of hospitalised patients in central Australia Free

Abstract

Summary

Single specimens of diarrhoeal stool from 676 patients, mostly aboriginals aged less than 5 years, admitted to Alice Springs Hospital, central Australia, for diarrhoea between Sept. 1988 and Feb. 1989, were examined for spp. by culture on a blood-free medium with selective supplement (BFM; Oxoid) and blood agar overlaid with a membrane filter (FM). spp. were isolated on either BFM or FM or both from 225 patients. spp. were isolated on BFM alone from 75 patients and on FM alone from 213 patients ( < 0.001; χ test). Most campylobacters isolated on BFM were . All subsp. , all “” except one, all subsp. and several uncharacterised isolates were isolated on FM only. was isolated on BFM but not FM from several patients, and . Serotyping of and isolated from both media showed the serotypes recovered from the two media to be different in some patients. In some patients concurrent infection with several species or serotypes (up to five) of , or both, was shown for the first time by the use of FM. We conclude that the use in combination of a selective medium and a non-selective medium with a filtration technique are better than either medium alone for the isolation of spp.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1992
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-3-176
1992-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/3/medmicro-37-3-176.html?itemId=/content/journal/jmm/10.1099/00222615-37-3-176&mimeType=html&fmt=ahah

References

  1. Blaser M. J., Reller L. B. Campylobacter enteritis. N Engl J Med 1981; 305:1444–1452
     [Google Scholar]
  2. Blaser M. J., Taylor D. N., Feldman R. A. Epidemiology of Campyl obacter jejuni infections. Epidemiol Rev 1983; 5:157–176
     [Google Scholar]
  3. Blaser M. J., Berkowitz I. D., LaForce F. M., Cravens J., Reller L. B., Wang W.-L. L. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med 1979; 91:179–185
     [Google Scholar]
  4. Bolton F. J., Robertson L. A selective medium for isolating Campylobacter jejuni/coli. J Clin Pathol 1982; 35:462–467
     [Google Scholar]
  5. Goossens H., DeBoeck M., Butzler J. P. A new selective medium for the isolation of Campylobacter jejuni from human faeces. Eur J Clin Microbiol 1983; 2:389–394
     [Google Scholar]
  6. Bolton F. J., Hutchinson D. N., Parker G. Reassessment of selective agars and filtration techniques for isolation of Campylobacter species from faeces. Eur J Clin Microbiol Infect Dis 1988; 7:155–160
     [Google Scholar]
  7. Goossens H., Vlaes L., DeBoeck M. Is “Campylobacter upsaliensis” an unrecognised cause of human diarrhoea?. Lancet 1990; 335:584–586
     [Google Scholar]
  8. Steele T. W., Sangster N., Lanser J. A. DNA relatedness and biochemical features of Campylobacter spp. isolated in Central and South Australia. J Clin Microbiol 1985; 22:71–74
     [Google Scholar]
  9. Steele T. W., McDermott S. N. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from faeces. Pathology 1984; 16:263–265
     [Google Scholar]
  10. Bolton F. J., Hutchinson D. N., Coates D. Blood-free selective medium for isolation of Campylobacter jejuni from feces. J Clin Microbiol 1984; 19:169–171
     [Google Scholar]
  11. Karmali M. A., Simor A. E., Roscoe M., Fleming P. C., Smith S. S., Lane J. Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces. J Clin Microbiol 1986; 23:456–459
     [Google Scholar]
  12. Tee W., Anderson B. N., Ross B. C., Dwyer B. Atypical campyl obacters associated with gastroenteritis. J Clin Microbiol 1987; 25:1248–1252
     [Google Scholar]
  13. Costas M., Owen R. J., Jackman P. J. H. Classification of Campylobacter sputorum and allied Campylobacters based on numerical analysis of electrophoretic protein patterns. Syst Appl Microbiol 1987; 9:125–131
     [Google Scholar]
  14. Lastovica A. J., Marshall R. B., Penner J. L. Cam€pylobacter sero-typing on shipped antigen extracts. Lancet 1986; 1:320
     [Google Scholar]
  15. Penner J. L., Hennessy J. N., Congi R. V. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol 1983; 2:378–383
     [Google Scholar]
  16. World Health Organization Programme for control of diarrhoeal diseases [CDD/83.3]. In Manual for laboratory investigations of acute enteric infections Geneva, World Health Organization; 1983
     [Google Scholar]
  17. Herrmann J. E., Perron D. M., Blacklow N. R. Enzyme immunoassay with monoclonal antibodies for the detection of rotavirus in stool specimens. J Infect Dis 1985; 152:830–832
     [Google Scholar]
  18. Herrmann J. E., Perron-Henry D. M., Blacklow N. R. Antigen detection with monoclonal antibodies for the diagnosis of adenovirus gastroenteritis. J Infect Dis 1987; 155:1167–1171
     [Google Scholar]
  19. Herrmann J. E., Nowak N. A., Perron-Henry D. M., Hudson R. W., Cubitt W. D., Blacklow N. R. Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies. J Infect Dis 1990; 161:226–229
     [Google Scholar]
  20. Simor A. E., Wilcox L. Enteritis associated with Campylobacter laridis . J Clin Microbiol 1987; 25:10–12
     [Google Scholar]
  21. Edmonds P., Patton C. M., Griffin P. M. Campylobacter hyointestinalis associated with human gastrointestinal disease in the United States. J Clin Microbiol 1987; 25:685–691
     [Google Scholar]
  22. Harvey S. M., Greenwood J. R. Probable Campylobacter fetus subsp. fetus gastroenteritis. J Clin Microbiol 1983; 18:1278–1279
     [Google Scholar]
  23. Steele T. W., Owen R. J. Campylobacter jejuni subsp. doylei subsp. nov., a subspecies of nitrate-negative Campylobacters isolated from human clinical specimens. IntJ Syst Bacteriol 1988; 38:316–318
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-3-176
Loading
Comparison of a blood-free medium and a filtration technique for the isolation of Campylobacter spp. from diarrhoeal stools of hospitalised patients in central Australia
J. Med. Microbiol. 37, 176 (1992); https://doi.org/10.1099/00222615-37-3-176
/content/journal/jmm/10.1099/00222615-37-3-176
/content/journal/jmm/10.1099/00222615-37-3-176
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error