1887

Abstract

Summary

Genetic variability among 41 penicillinase non-producing strains, isolated in Spain, with different levels of resistance to penicillin was investigated by multilocus enzyme electrophoresis. Based on the results obtained by analysis at seven enzyme loci, the strains were separated into 17 electrophoretic types. The average number of alleles/enzyme locus was 2.85; the mean genetic diversity/locus was 0.49 for individual isolates and 0.516 for electrophoretic types. The results showed that these gonococcal strains were, genetically, a highly variable group of organisms.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-2-96
1992-08-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/2/medmicro-37-2-96.html?itemId=/content/journal/jmm/10.1099/00222615-37-2-96&mimeType=html&fmt=ahah

References

  1. Dowsett E. G. Penicillin–resistant gonococci. Lancet 1980; 2:202 (letter)
    [Google Scholar]
  2. Rice R. J., Biddle J. W., JeanLouis Y. A., DeWitt W. E., Blount J. H., Morse S. A. Chromosomally–mediated resistance in Neisseria gonorrhoeae in the United States: results of surveillance and reporting, 1983–4. J Infect Dis 1986; 153:340–345
    [Google Scholar]
  3. Van Klingeren B. Ansink–Schipper M. C. Doornbos L. Surveillance of the antibiotic susceptibility of non–penicillinase producing Neisseria gonorrhoeae in the Netherlands from 1983 to 1986. J Antimicrob Chemother 1988; 21:737–744
    [Google Scholar]
  4. Nogueira J. M., Palomares J. C., Vázquez J. A. Epidemiología del gonococo en España. Rev Iberoam Enf Trans Sex 1990; 4:9–15
    [Google Scholar]
  5. Maier T. W., Zubrzycki L., Coyle M. B., Chila M., Warner P. Genetic analysis of drug resistance in Neisseria gonorrhoeae; production of increased resistance by the combination of two antibiotic resistance loci. J Bacteriol 1975; 124:834–842
    [Google Scholar]
  6. Sparling P. F. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance. J Bacteriol 1966; 92:1364–1371
    [Google Scholar]
  7. Fenoll A., Berron S., Vazquez J. A. Analysis of penicillinase–producing Neisseria gonorrhoeae isolates in Madrid (Spain) from 1983–85. Epidemiol Infect 1987; 99:455–A62
    [Google Scholar]
  8. Bygdeman S. Polyclonal and monoclonal antibodies applied to the epidemiology of gonococcal infection. In Young H., McMillan A. (eds) Immunological diagnosis of sexually transmitted diseases New York: Marcel Dekker Inc; 1987117–165
    [Google Scholar]
  9. White L. A., Kellogg D. S. Neisseria gonorrhoeae identification in direct smears by a fluorescent antibody–counterstain method. Appl Microbiol 1965; 13:171–174
    [Google Scholar]
  10. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and sys– tematics. Appl Environ Microbiol 1986; 51:873–884
    [Google Scholar]
  11. Nei M. Estimation of average heterozygosity and genetic distance from a small sample of individuals. Genetics 1978; 89:583–590
    [Google Scholar]
  12. Hook E. W., Holmes K. K. Gonococcal infections. Ann Intern Med 1985; 102:229–243
    [Google Scholar]
  13. Reyn A., Komer B., Bentzon M. W. Effects of penicillin, strep tomycin, and tetracycline on Neisseria gonorrhoeae isolated in 1944 and in 1957. Br J Vener Dis 1958; 34:227–239
    [Google Scholar]
  14. Mendelman P. M., Caugant D. A., Kalaitzoglou G. Genetic diversity of penicillin G–resistant Neisseria meningitidis from Spain. Infect Immun 1989; 57:1025–1029
    [Google Scholar]
  15. Porras O., Caugant D. A., Gray B., Lagergard T., Levin B. R. Svanborg–Eden C.Difference in structure between type b and nontypable Haemophilus influenzae populations. Infect Immun 1986; 53:79–89
    [Google Scholar]
  16. Ochman H., Whittman T. S., Caugant D. A., Selander R. K. Enzyme polymorphism and genetic population structure in Escherichia coli and Shigella . J Gen Microbiol 1983; 129:2715–2726
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-2-96
Loading
/content/journal/jmm/10.1099/00222615-37-2-96
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error