1887

Abstract

Summary

Enterotoxigenic (ETEC) strain P2200 of porcine origin possessed eight possibly plasmid-determined characters (K88 Raf HlyColSmTcSuSTa) and six plasmid DNA bands of 4.2–93 kb. Analysis of the spontaneous loss of characters and the results of matings with other strains revealed that the K88, Raf, Hly, Sm, Tc and Su characters could be transferred, and that the presence of the K88 and Raf characters was associated with an 83-kb plasmid. The presence and location of the STaI gene was investigated in several ETEC strains of bovine or porcine origin. Hybridisation with a non-radioactive polynucleotide probe associated the STaI gene with a plasmid in each strain; these plasmids were of 32–142 kb. In contrast, plasmids from a P2200 STa variant and plasmids from two STa variants of the bovine ETEC strain B41* (strain B41 obtained from a different source) did not hybridise with the probe. One of the B41*STa variants had lost the STa plasmid, whereas the second variant retained a plasmid of the same size which did not hybridise. In contrast, a third B41*STa variant retained a plasmid of the same size that still hybridised with the STaI probe. Plasmid DNA restriction fragment analysis, followed by hybridisation with the STaI probe, showed that the STaI gene was associated with 8.3-, 6.8- and 3.5-kb plasmid fragments in strain B41, and with 4.9-, 6.8- and 3.5-kb plasmid fragments in strain B41*, following digestion with RI, HI, or RI + HI, respectively. The STaI probe hybridised also with 12.2-kb RI and 4.6-kb HI fragments of the plasmid from the third B41*STa variant, that unexpectedly had given an initial positive hybridisation result. Different plasmid restriction fragment profiles were seen for strains B41 and B41*, the B41*STa variants and the transconjugant strains, thereby providing further evidence that molecular rearrangements of these plasmids can occur spontaneously.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-2-141
1992-08-01
2022-11-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/2/medmicro-37-2-141.html?itemId=/content/journal/jmm/10.1099/00222615-37-2-141&mimeType=html&fmt=ahah

References

  1. Gaastra W., De Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev 1982; 46:129–161
    [Google Scholar]
  2. Klemm P. Fimbrial adhesins of Escherichia coli . Rev Infect Dis 1985; 7:321–340
    [Google Scholar]
  3. De Graaf F. K., Mooi F. R. The fimbrial adhesins of Escherichia coli. Adv Microb Physiol 1986; 28:65–143
    [Google Scholar]
  4. Schifferli D. M., Beachey E. H., Taylor R. K. The 987P fimbrial gene cluster of enterotoxigenic Escherichia coli is plasmid encoded. Infect Immun 1990; 58:149–156
    [Google Scholar]
  5. Thompson M. R. Escherichia coli heat-stable enterotoxins and their receptors. PatholImmunopathol Res 1987; 6:103–116
    [Google Scholar]
  6. Gyles C. L., So M., Falkow S. The enterotoxin plasmids of Escherichia coli. J Infect Dis 1974; 130:40–49
    [Google Scholar]
  7. Elwell L. P., Shipley P. L. Plasmid-mediated factors associated with virulence of bacteria to animals. Annu Rev Microbiol 1980; 34:465–196
    [Google Scholar]
  8. Smith H. R., Scotland S. M., Rowe B. Genetics of Escherichia coli virulence. In Sussman M. (ed) The virulence of Escherichia coli London: Academic Press; 1985228–269
    [Google Scholar]
  9. Bertin A. Virulence factors of enterotoxigenic Escherichia coli studied in the infant mouse model. Ann Rech Vet 1983; 14:169–182
    [Google Scholar]
  10. Duchet-Suchaux M. F., Bertin A. M., Menanteau P. S. Susceptibility of Chinese Meishan and European Large White pigs to enterotoxigenic Escherichia coli strains bearing colonization factor K88, 987P, K99, or F41. Am J Vet Res 1991; 52:40–44
    [Google Scholar]
  11. Smith H. W. The bacteriology of the alimentary tract of domestic animals suffering from Escherichia coli infection. Ann NY AcadSci 1971; 176:110–125
    [Google Scholar]
  12. Bertin A. F41 antigen as a virulence factor in the infant mouse model of Escherichia coli diarrhoea. J Gen Microbiol 1985; 131:3037–3045
    [Google Scholar]
  13. Duchet-Suchaux M. Le souriceau, modèle d’étude de la diarrhée colibacillaire. Ann Microbiol (Inst Pasteur) 1980; 131B:239–250
    [Google Scholar]
  14. Duchet-Suchaux M., Le Maitre C., Bertin A. Differences in susceptibility of inbred and outbred infant mice to enterotoxigenic Escherichia coli of bovine, porcine and human origin. J Med Microbiol 1990; 31:185–190
    [Google Scholar]
  15. Duchet-Suchaux M. Protective antigens against enterotoxigenic Escherichia coli O101: K99, F41 in the infant mouse diarrhoea model. Infect Immun 1988; 56:1364–1370
    [Google Scholar]
  16. Fredericq P. Actions antibiotiques reciproques chez les enterobacteriaceae. Rev Beige Pathol Med Exp 1948; 19: Suppl IV1–107
    [Google Scholar]
  17. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis 1972; 125:407–411
    [Google Scholar]
  18. Kado C. L., Liu S.-T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145:1365–1373
    [Google Scholar]
  19. Davis L. G., Dibner M. D., Battey J. F. Basic methods in molecular biology. Amsterdam: Elsevier Science Publishing Co; 1986
    [Google Scholar]
  20. Birnboim H. C., Doly J. A. rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
    [Google Scholar]
  21. Holmes D. S., Quigley M. A. rapid boiling method for the preparation ofbacterialplasmids. AnalBiochem 1981; 114:193–197
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning. A laboratory manual. New York: Cold Spring Harbor Laboratory; 1982
    [Google Scholar]
  23. Bialoskowa-Hobrzanska H. Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes. J Clin Microbiol 1987; 25:338–341
    [Google Scholar]
  24. Casey T. A., Moon H. W. Genetic characterization and virulence of enterotoxigenic Escherichia coli mutants which have lost virulence gene in vivo . Infect Immun 1990; 58:4156–4158
    [Google Scholar]
  25. So M., Heffron F., McCarty B. J. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature 1979; 277:453–456
    [Google Scholar]
  26. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol 1976; 127:1529–1537
    [Google Scholar]
  27. So M., Boyer H. W., Betlach M., Falkow S. Molecular cloning of an Escherichia coli plasmid determinant that encodes for the production of heat-stable enterotoxin. J Bacteriol 1976; 128:463–472
    [Google Scholar]
  28. Sommerfelt H., Grewal H. M. S., Bhan M. K. Simplified and accurate nonradioactive polynucleotide gene probe assay for identification of Escherichia coli . J Clin Microbiol 1990; 28:49–54
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-2-141
Loading
/content/journal/jmm/10.1099/00222615-37-2-141
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error