1887

Abstract

Summary

Cytopathic effects of broth-culture filtrates from eight clinical isolates and one reference strain of on three cultured mammalian cell lines were investigated. All the strains, including NCTC 11637, produced cytotoxic factors that caused intracellular vacuolation on these cell lines. AGS and SflEp cells were more sensitive than HEp-2 cells. To examine the role of urease in the cytotoxic effect, a urease-negative mutant was produced. Filtrates from both wild-type and mutant strains produced similar vacuolation on Sfl Ep cells in the absence of urea, suggesting that produces a cytotoxic substance other than urease. In contrast, ammonia alone, or jack bean urease with urea, also induced rounding and detachment of Sfl Ep cells, whereas ammonium salts induced the production of small vacuoles. The combination of the broth filtrate of the wild-type strain and urea induced vacuolation followed by rounding and detachment of Sfl Ep cells. Evidence is presented that the latter changes are due to ammonia produced during incubation. Nevertheless, the amounts produced were less than that needed to induce cytopathic effects by itself. These results suggest that the cytotoxic substance induces intracellular vacuolation, and that the vacuolated cells are more susceptible to killing by ammonia. Thus both the cytotoxic substance and urease may contribute to the lethal cytotoxicity of

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-2-118
1992-08-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/2/medmicro-37-2-118.html?itemId=/content/journal/jmm/10.1099/00222615-37-2-118&mimeType=html&fmt=ahah

References

  1. Goodwin C. S., Armstrong J. A., Chilvers T. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb, nov., respectively. Int J Syst Bacteriol 1989; 39:397–405
    [Google Scholar]
  2. Blaser M. J. Helicobacter pylori and the pathogenesis of gas troduodenal inflammation. J Infect Dis 1990; 161:626–633
    [Google Scholar]
  3. Blaser M. J. Epidemiology and pathophysiology of Campy lobacter pylori infections. Rev Infect Dis 1990; 12: Suppl 1S99–S106
    [Google Scholar]
  4. Marshall B. J. Campylobacter pylori: its link to gastritis and peptic ulcer disease. Rev Infect Dis 1990; 12: Suppl 1S87–S93
    [Google Scholar]
  5. Marshall B. J., Armstrong J. A., McGechie D. B., Glancy R. J. Attempt to fulfil Koch’s postulates for pyloric Campylobacter. Med J Aust 1985; 142:436–439
    [Google Scholar]
  6. Morris A., Nicholson G. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol 1987; 82:192–199
    [Google Scholar]
  7. Chen X. G., Correa P., Offerhaus J. Ultrastructure of the gastric mucosa harboring campylobacter-like organisms. Am J Clin Pathol 1986; 86:575–582
    [Google Scholar]
  8. Fiocca R., Villani L., De Giacomo C. Morphological evidence of Campylobacter pylori pathogenicity in chronic gastritis and peptic ulcer. Acta Gastroenterol Belg 1989; 52:324–335
    [Google Scholar]
  9. Hupertz V., Czinn S. Demonstration of a cytotoxin from Campylobacter pylori. Eur J Clin Microbiol Infect Dis 1988; 7:576–578
    [Google Scholar]
  10. Leunk R. D., Johnson P. T., David B. C., Kraft W. G., Morgan D. R. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori . J Med Microbiol 1988; 26:93–99
    [Google Scholar]
  11. Leunk R. D., Ferguson M. A., Morgan D. R., Low D. E., Simor A. E. Antibody to cytotoxin in infection by Helicobacter pylori . J Clin Microbiol 1990; 28:1181–1184
    [Google Scholar]
  12. Figura N., Guglielmetti P., Rossolini A. Cytotoxin pro duction by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. J Clin Microbiol 1989; 27:225–226
    [Google Scholar]
  13. Cover T. L., Dooley C. P., Blaser M. J. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect Immun 1990; 58:603–610
    [Google Scholar]
  14. Barer M. R., Elliot T. S. J., Berkeley D., Thomas J. E., Eastham E. J. Cytopathic effects of Campylobacter pylori urease. J Clin Pathol 1988; 41:597 (letter)
    [Google Scholar]
  15. Goodwin C. S., Armstrong J. A. Microbiological aspects of Helicobacter pylori (Campylobacter pylori). Eur J Clin Microbiol Infect Dis 1990; 9:1–13
    [Google Scholar]
  16. Smoot D. T., Mobley H. L. T., Chippendale G. R., Lewison J. F., Resau J. H. Helicobacter pylori urease activity is toxic to human gastric epithelial cells. Infect Immun 1990; 58:1992–1994
    [Google Scholar]
  17. Xu J., Goodwin C. S., Cooper M., Robinson J. Intracellular vacuolisation caused by the urease of Helicobacter pylori . J Infect Dis 1990; 161:1302–1304
    [Google Scholar]
  18. Nakazawa T., Ishibashi M., Konishi H. Hemagglutination activity of Campylobacter pylori . Infect Immun 1989; 57:989–991
    [Google Scholar]
  19. Clowes R. R., Hayes W. Experiments in microbial genetics. New York: John Wiley and Sons; 1968187
    [Google Scholar]
  20. Kitajima K., Miyamura S. Direct determination of blood ammonia by Berthelot’s coloration of protein-free supernatant obtained by Folin-Wu deproteinization. Bull Yamaguchi Med Sch 1968; 15:207–217
    [Google Scholar]
  21. Mobley H. L. T., Cortesia M. J., Rosenthal L. E., Jones B. D. Characterization of urease from Campylobacter pylori . J Clin Microbiol 1988; 26:831–836
    [Google Scholar]
  22. Cover T. L., Puryar W., Perez-Perez G. I., Blaser M. J. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect Immun 1991; 59:1264–1270
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-2-118
Loading
/content/journal/jmm/10.1099/00222615-37-2-118
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error