Protection conferred on mice by combinations of monoclonal antibodies directed against outer-membrane proteins or smooth lipopolysaccharide of Brucella
The effect of monoclonal antibodies (MAbs) injected alone or in combination on brucella splenic infection in CD-1 mice was tested 7 and 21 days after a challenge with virulent Brucella abortus 544. Passive immunisation of mice with anti-25–27-kDa MAb alone, or mixed with protective anti-16.5 and anti-36–38-kDa MAbs, or with MAbs of the same specificity which were previously demonstrated to have no activity on CD-1 mice, produced a significant reduction of spleen counts of B. abortus (p < 0.01). Other combinations of MAbs did not reduce splenic infection in comparison with the untreated control group. BALB/c mice were used to test the possible interference of the immune response of CD-1 mice against MAbs that were produced in BALB/c mice. No reduction of splenic infection was shown with anti-25–27-or-36–38-kDa MAbs, whereas anti-lipopolysaccharide (LPS) MAb which was produced in CBA mice was effective. Combination of anti-protein MAbs with the anti-LPS MAb produced only the effect of the anti-LPS MAb at 7 and 21 days after challenge.
PavlovH.,
HogarthM.,
McKenzieI. F. C.,
CheersC.In vivo and in vitro effects of monoclonal antibody to Ly antigens on immunity to infection. Cell Immunol1982; 71:127–138
CloeckaertA.,
JacquesI.,
BosserayN. Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella
. J Med Microbiol1991; 34:175–180
LimetJ.,
PlommetA.-M.,
DubrayG.,
PlommetM. Immunity conferred upon mice by anti-LPS monoclonal antibodies in murine brucellosis. Ann Inst Pasteur Immunol1987; 138:417–424
LimetJ. N.,
BosserayN.,
Garin-BastujiB.,
DubrayG.,
PlommetM. Humoral immunity in mice mediated by monoclonal antibodies against the A and M antigens of Brucella
. J Med Microbiol1989; 30:37–43
PhillipsM.,
DeyoeB. L.,
CanningP. C. Protection of mice against Brucella abortus infection by inoculation with monoclonal antibodies recognizing Brucella O-antigen. Am J Vet Res1989; 50:2158–2161
MontarazJ. A.,
WinterA. J.,
HunterD. M.,
SowaB. A.,
WuA. M.,
AdamsL. G. Protection against Brucella abortus in mice with O-polysaccharide-specific monoclonal antibodies. Infect Immun1986; 51:961–963
AltonG. G.,
JonesL. M.,
AngusR. D.,
VergerJ. M. Techniques for the brucellosis laboratory. Paris: Institut National de la Recherche Agronomique; 198834–42
PlommetM.,
BosserayN. Le contrôle des vaccins anti-brucelliques par dénombrement des Brucella dans la rate de souris, vaccinées ou non, inoculees par voie intra-péritoneale. J Biol Stand1977; 5:261–274
BosserayN.,
PlommetA.-M.,
PlommetM. Theoretical, practical and statistical basis for a general control method of activity for anti-Brucella vaccines. Dev Biol Stand1984; 56:257–270
BosserayN.,
PlommetM. Transformation normalisant la distribution du nombre de Brucella dans la rate de souris inoculees par voie intrapéritoneale. J Biol Stand1976; 4:341–351
SvennerholmA.-M.,
WenneråsC.,
HolmgrenJ.,
McConnellM. M.,
RoweB. Roles of different coli surface antigens of colonization factor antigen II in colonization by and protective immunogenecity of enterotoxigenic Escherichia coli in rabbits. Infect Immun1990; 58:341–346
Protection conferred on mice by combinations of monoclonal antibodies directed against outer-membrane proteins or smooth lipopolysaccharide of Brucella