1887

Abstract

Summary

Clinical (66) and reference (5) strains of pigmented gram-negative anaerobic bacilli, identified as (47), (1), (8), (12), (1) and (2), were examined by pyrolysis mass spectrometry (PMS) and in conventional tests. Numerical classification based on conventional test reaction patterns (CTRPs) resolved five clusters, four comprising strains identified as , and respectively, and one comprising strains identified as and . Numerical classification based on PMS showed a similar division, with decreasing homogeneity of chemical composition in the order , which agreed with the order of homogeneity in CTRPs. PMS clusters corresponding to the genus were clearly distinct from those of the genus . PMS and CTRP classification disagreed on cluster membership for six strains. PMS identification from blind challenge sets was in agreement with conventional identification for 64 of 67 strains.

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1992-07-01
2022-05-22
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References

  1. Oliver W. W., Wherry W. B. Notes on some bacterial parasites of the human mucous membranes. J Infect Dis 1921; 28:341–345
    [Google Scholar]
  2. Moore W. E. C., Holdeman L. V. New names and combinations in the genera Bacteroides Castellani and Chalmers, Fusobacterium Knorr, Eubacterium Prevot, Propionibacterium Delwich, and Lactobacillus OrlaJensen. Int J Syst Bac– teriol 1973; 23:69–74
    [Google Scholar]
  3. Finegold S. M., Barnes E. M. Report of the ICSB Taxonomic Subcommittee on Gram–negative Anaerobic Rods. Proposal that the saccharolytic and asaccharolytic strains at present classified in the species Bacteroides melaninogenicus (Oliver and Wherry) be reclassified in two species as Bacteroides melaninogenicus and Bacteroides asaccharolyticus . Int J Syst Bacteriol 1977; 27:388–391
    [Google Scholar]
  4. Coykendall A. L., Kaczmarek F. S., Slots J. Genetic heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) Finegold and Barnes 1977 (Approved Lists, 1980) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov. Int J Syst Bacteriol 1980; 30:559–564
    [Google Scholar]
  5. Shah H. N., Collins M. D. Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas . Int J Syst Bacteriol 1988; 38:128–131
    [Google Scholar]
  6. Shah H. N., Collins M. D. Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int J Syst Bacteriol 1989; 39:85–87
    [Google Scholar]
  7. Shah H. N., Collins M. D. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides . Int J Syst Bacteriol 1990; 40:205–208
    [Google Scholar]
  8. Socransky S. S., Gibbons R. J., Dale A. C., Bortnick L., Rosenthal E., MacDonald J. B. The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Arch Oral Biol 1963; 8:275–280
    [Google Scholar]
  9. Slots J. Microflora in the healthy gingival sulcus in man. Scand J Dent Res 1977; 85:247–254
    [Google Scholar]
  10. Duerden B. I. The isolation and identification of Bacteroides spp. from the normal human gingival flora. J Med Microbiol 1980; 13:89–101
    [Google Scholar]
  11. Gorbach S. L., Menda K. B., Thadepalli H., Keith L. Anaerobic microflora of the cervix in healthy women. Am J Obstet Gynecol 1973; 117:1053–1055
    [Google Scholar]
  12. Drasar B. S., Shiner M., McLeod G. M. Studies on the intestinal flora. Gastroenterology 1969; 56:71–79
    [Google Scholar]
  13. Slots J. Subgingival microflora and periodontal disease. J Clin Periodontol 1979; 6:351–382
    [Google Scholar]
  14. Finegold S. M. Anaerobic bacteria in human disease. New York: Academic Press; 1977
    [Google Scholar]
  15. Van Steenbergen T. J. M., van Winkelhoff A. J., van der Velden U., de Graaff J. Taxonomy, virulence and epidemiology of black– pigmented Bacteroides species in relation to oral infections. Infection 1989; 17:194–196
    [Google Scholar]
  16. Finegold S. M. Intra-abdominal, genitourinary, skin and soft tissue infections due to non–sporing anaerobic bacteria. In Phillips I., Sussman M. (eds) Infection with non–sporing bacteria Edinburgh: Churchill Livingstone; 1974160
    [Google Scholar]
  17. Thadepalli H., Gorbach S. L., Keith L. Anaerobic infections of the female genital tract: bacteriologic and therapeutic aspects. Am J Obstet Gynecol 1973; 117:1034–1040
    [Google Scholar]
  18. Slots J. Importance of black pigmented Bacteroides in human periodontal disease. In Genco R. J., Mergenhagan S. E. (eds) Host parasite interactions in periodontal diseases Washington DC: American Society for Microbiology; 198227
    [Google Scholar]
  19. Van Winkelhoff A. J., van Steenbergen T. J. M., de Graaff J. The role of black pigmented Bacteroides in human oral infections. J Clin Periodontol 1988; 15:145–155
    [Google Scholar]
  20. Slots J., Listgarten M. A. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontal 1988; 15:85–93
    [Google Scholar]
  21. Loesche W. J., Syed S. A., Morrison E. C., Laughton B., Grossman N. S. Treatment of periodontal infections due to anaerobic bacteria with short–term treatment with metronidazole. J Clin Periodontol 1981; 8:29–44
    [Google Scholar]
  22. Duerden B. I., Goodwin L., O’Neil T. C. A. Identification of Bacteroides species from adult periodontal disease. J Med Microbiol 1987; 24:133–137
    [Google Scholar]
  23. Duerden B. I., Holbrook W. P., Collee J. G., Watt B. The characterization of clinically important gram–negative anaerobic bacilli by conventional bacteriological tests. J Appl Bacteriol 1976; 40:163–188
    [Google Scholar]
  24. Duerden B. I., Collee J. G., Brown R., Deacon A. G., Holbrook W. P. A scheme for the identification of clinical isolates of gram-negative anaerobic bacilli by conventional bacteriological tests. J Med Microbiol 1980; 13:231–245
    [Google Scholar]
  25. Rotimi V. O., Faulkner J., Duerden B. I. Rapid methods for identification of clinical isolates of gram–negative anaerobic bacilli. Med Lab Sci 1980; 37:331–339
    [Google Scholar]
  26. Rawlinson A. The clinical and microbial changes during the treatment of periodontal disease with special reference to anaerobic gram–negative organisms. MDS thesis University of Sheffield; 1990
    [Google Scholar]
  27. Gutteridge C. S., Vallis L., MacFie H. J. H. Numerical methods in the classification of micro–organisms by pyrolysis mass spectrometry. In Goodfellow M., Jones D., Priest F. G. (eds) Computer–assisted bacterial systematics. (Special publications of the Society for General Microbiology 15.) London: Academic Press; 1985369–402
    [Google Scholar]
  28. Magee J. T. Whole organism fingerprinting. In Goodfellow M., O’Donnell K. (eds) The new bacterial taxonomy London: Academic Press; 1991 in press
    [Google Scholar]
  29. Magee J. T., Hindmarch J. M., Bennett K. W., Duerden B. I., Aries R. E. A pyrolysis mass spectrometry study of fusobacteria. J Med Microbiol 1989; 28:227–236
    [Google Scholar]
  30. Duerden B. I., Eley A., Goodwin L., Magee J. T., Hindmarch J. M., Bennett K. W. A comparison of Bacteroides ureolyticus isolates from different clinical sources. J Med Microbiol 1989; 29:63–73
    [Google Scholar]
  31. Magee J. T., Yousefi–Mashouf R., Hindmarch J. M., Duerden B. I. A pyrolysis mass spectrometry study of the non–pigmented Prevotella species. J Med Microbiol 1992 (in press)
    [Google Scholar]
  32. Holbrook W. P., Ogston S. A., Ross P. W. A method for the isolationof Bacteroides melaninogenicus from the human mouth. J Med Microbiol 1978; 11:203–207
    [Google Scholar]
  33. Casals J. B., Pringler N. Diagnostic tablets for bacterial identification. Rosco Diagnostica, Taastrup, Denmark 1987
    [Google Scholar]
  34. Slots J., Genco R. J. Direct hemagglutination technique for differentiating Bacteroides asaccharolyticus oral strains from nonoral strains. J Clin Microbiol 1979; 10:371–373
    [Google Scholar]
  35. Kaczmarek F. S., Coykendall A. L. Production of phenylacetic acid by strains of Bacteroides asaccharolyticus and Bacteroides gingivalis sp. nov. J Clin Microbiol 1980; 12:288–290
    [Google Scholar]
  36. Deacon A. G., Duerden B. I., Holbrook W. P. Gas–liquid chromatographic analysis of metabolic products in the identification of Bacteroidaceae of clinical interest. J Med Microbiol 1978; 11:81–99
    [Google Scholar]
  37. Brazier J. S. A note on ultra–violet red fluorescence of anaerobic bacteria. J Appl Bacteriol 1986; 60:121–126
    [Google Scholar]
  38. Rohlf F. J. Congruence of larval and adult classifications in Aedes (Diptera, Culicidae). Syst Zool 1963; 12:97–117
    [Google Scholar]
  39. Sokal R. R., Michener C. D. A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 1958; 38:1409–1438
    [Google Scholar]
  40. Hindmarch J. M., Magee J. T., Hadfield M. A., Duerden B. I. A pyrolysis-mass spectrometry study of Corynebacterium spp. J Med Microbiol 1990; 31:137–149
    [Google Scholar]
  41. Winstanley T. G., Magee J. T., Limb D. I. A numerical taxonomic study of the “Streptococcus milleri group” based upon conventional phenotypic tests and pyrolysis mass spectrometry. J Med Microbiol 36:149–155
    [Google Scholar]
  42. Wieten G., Haverkamp J., Meuzelaar H. L. C., Engel H. W. B., Berwald L. G. Pyrolysis mass spectrometry: a new method to differentiate between the mycobacteria of the “Tuberculosis complex” and other mycobacteria. J Gen Microbiol 1981; 122:109–118
    [Google Scholar]
  43. Wieten G., Haverkamp J., Engel H. W. B., Berwald L. G. Application of pyrolysis mass spectrometry to the classification and identification of mycobacteria. Rev Infect Dis 1981; 3:871–877
    [Google Scholar]
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