1887

Abstract

Summary

Lactobacilli isolated from the vaginas of healthy women (39 strains) and from the vaginal discharge of women with bacterial vaginosis (15 strains) were investigated for their binding to I-fibronectin. Nine of the 54 strains bound fibronectin at pH 7.2. The binding capacity of these nine strains was about the same as that observed with Cowan 1. The binding was specific; an excess of unlabelled fibronectin or its amino-terminal 29-kDa fragment effectively competed for binding, whereas bovine serum albumin, human IgG and orosomucoid did not. Incubation of lactobacilli with fibronectin for different periods revealed a time-dependent increase in binding. Lowering the pH to 4•0 increased the binding capacity of all of the lactobacilli tested; binding occurred with strains that had previously failed to bind at pH 7•2. The increased binding of lactobacilli to fibronectin at a low pH may play a role in the maintenance of the ecological balance of the vagina.

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1992-07-01
2024-04-24
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References

  1. Eschenbach D. A., Bekassy S., Blackwell A., Ekgren J., Hallen A., Wathne B. The diagnosis of bacterial vagVinosis. Scand J Urol Nephrol 1985 Suppl 86:260–261
    [Google Scholar]
  2. Hill G. B., Eschenbach D. A., Holmes K. K. Bacteriology of vagina. Scand J Urol Nephrol 1985 Suppl 86:23–39
    [Google Scholar]
  3. Skarin A., Mardh P.-A. Comma-shaped bacteria associated with vaginitis. Lancet 1982; 1:342
    [Google Scholar]
  4. Wylie J. G., Henderson A. Identity of glycogen-fermenting ability of lactobacilli isolated from the vagina of pregnant women. J Med Microbiol 1969; 2:363–366
    [Google Scholar]
  5. Mardh P.-A., Soltesz L. V. In vitro interactions between lacto bacilli and other microorganisms occurring in the vaginal flora. Scand J Infect Dis 1983 Suppl 40:47–51
    [Google Scholar]
  6. Eschenbach D. A., Davick P. R., Williams B. L. Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J Clin Microbiol 1989; 27:251–256
    [Google Scholar]
  7. Cohen M. S., Black J. R., Proctor R. A., Sparling P. F. Host defences and the vaginal mucosa: reevaluation. Scand J Urol Nephrol 1985 Suppl 86:13–22
    [Google Scholar]
  8. Mardh P.-A., Westrom L. Adherence of bacteria to vaginal epithelial cells. Infect Immun 1976; 13:661–666
    [Google Scholar]
  9. Sobel J. D., Schneider J., Kay D., Levison M. E. Adherence of bacteria to vaginal epithelial cells at various times in the menstrual cycle. Infect Immun 1981; 32:194–197
    [Google Scholar]
  10. Wood J. R., Sweet R. L., Catena A., Hadley W. K., Robbie M. In vitro adherence of Lactobacillus species to vaginal epithelial cells. Am J Obstet Gynecol 1985; 153:740–743
    [Google Scholar]
  11. Mosher D. F. Physiology of fibronectin. Annu Rev Med 1984; 35:561–575
    [Google Scholar]
  12. Proctor R. Fibronectin: a brief overview of its structure, function and physiology. Rev Infect Dis 1987; 9: Suppl 4S317–S321
    [Google Scholar]
  13. Hynes R. Molecular biology of fibronectin. Annu Rev Cell Biol 1985; 1:67–90
    [Google Scholar]
  14. Dunne W. M. Fibronectin: the biological Velcro. Clin Microbiol Newslett 1989; 11:57–59
    [Google Scholar]
  15. Espersen F., Clemmensen I. Isolation of fibronectin-binding protein from Staphylococcus aureus. Infect Immun 1982; 37:526–531
    [Google Scholar]
  16. Kuusela P., Vartio T., Vuento M., Myhre E. B. Attachment of staphylococci and streptococci on fibronectin, fibronectin fragments, and fibrinogen bound to solid phase. Infect Immun 1985; 50:77–81
    [Google Scholar]
  17. Switalski L. M., Ljungh A., Ryden C., Rubin K., Hook M., Wadstrom T. Binding of fibronectin to the surface of group A, group C, and group G streptococci isolated from human infections. Eur J Clin Microbiol Infect Dis 1982; 1:381–387
    [Google Scholar]
  18. Froman G., Switalski L. M., Faris A., Wadstrom T., Hook M. Binding of Escherichia coli to fibronectin. J Biol Chem 1984; 259:14899–14905
    [Google Scholar]
  19. Baloda S. B., Faris A., Froman G., Wadstrom T. Fibronectin binding to Salmonella strains. FEMS Microbiol Lett 1985; 28:1–5
    [Google Scholar]
  20. Thomas D. D., Baseman J. B., Alderete J. F. Enhanced levels of attachment of fibronectin-primed Treponema pallidum to extracellular matrix. Infect Immun 1986; 52:736–741
    [Google Scholar]
  21. Hunter W. M. Radioimmunoassay. In Weir D. M. (ed) Hand book of experimental immunology Oxford: Blackwell Scientific; 1978; 141–40
    [Google Scholar]
  22. Froman G., Switalski L. M., Speziale P., Hook M. Isolation and characterization of a fibronectin receptor from Staphylococcus aureus . J Biol Chem 1987; 262:6564–6571
    [Google Scholar]
  23. Antonas K. N., Yajko D. M., Hardley W. K. Assay of fibronectin in vaginal fluid and the role of fibronectin in bacterial colonization. Annual Meeting of the American Society of Microbiology, New Orleans 1983 Abstract no 1310
    [Google Scholar]
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