1887

Abstract

Summary

The iron uptake mechanisms of enteropathogenic (EPEC) were examined and compared with those of control strains. The incidence of aerobactin production was similar (39% and 37% respectively) in the two groups. The quantities of enterochelin produced by aerobactin-negative EPEC and control strains were similar, as were the quantities of enterochelin produced by aerobactin-positive EPEC and control strains. The ability to use haem or haemoglobin as an iron source in an iron-restricted environment was found in 80.4% and 60.8% of EPEC strains respectively, and in 76.6% and 56.6% of control strains. The ability of strains to use these compounds was not related to the production of enterochelin or aerobactin or to the production of haemolysins, and may be an important characteristic of bowel organisms. When growing in an iron-limited environment, the iron contained in haemoglobin was used in preference to ovotransferrin-bound iron. During periods of haemoglobin-stimulated growth, the enterochelin uptake system was shown to be fully expressed and may be involved in transport of haemoglobin-derived iron into the cell. Uptake of ovotransferrin-bound iron took place immediately upon exhaustion of haemoglobin-derived iron. The ability to use iron derived from haem compounds represents an alternative iron uptake mechanism for organisms growing in an iron-limited environment and allows greater flexibility during growth .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-37-1-15
1992-07-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/37/1/medmicro-37-1-15.html?itemId=/content/journal/jmm/10.1099/00222615-37-1-15&mimeType=html&fmt=ahah

References

  1. Levine M. M., Edelman R. Enteropathogenic Escherichia coli of classic serotypes associated with infant diarrhea; epidemiology and pathogenesis. Epidemiol Rev 1984; 6:31–51
    [Google Scholar]
  2. Levine M. M. Escherichia coli that cause diarrhea: Entero toxigenic, enteropathogenic, enteroinvasive, enterohem-morrhagic, and enteroadherent. J Infect Dis 1987; 155:377–389
    [Google Scholar]
  3. Law D. Virulence factors of enteropathogenic Escherichia coli . J Med Microbiol 1988; 26:1–10
    [Google Scholar]
  4. Griffiths E. The iron-uptake systems of pathogenic bacteria. In Bullen J. J., Griffiths E. (eds) Iron and infection Chichester, John Wiley; 198569–137
    [Google Scholar]
  5. Brown M. R. W., Williams P. The influence of environment on envelope properties affecting survival of bacteria in infection. Annu Rev Microbiol 1985; 39:527–556
    [Google Scholar]
  6. Ward K. H., Anwar H., Brown M. R. W., Wale J., Gowar J. Antibody response to outer-membrane antigens of Pseudomonas aeruginosa in human bum wound infection. J Med Microbiol 1988; 27:179–190
    [Google Scholar]
  7. Shand G. H., Anwar H., Kadurugamuwa J., Brown M. R. W., Silverman S. H., Melling J. In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun 1985; 48:35–39
    [Google Scholar]
  8. Bolin C. A., Jensen A. E. Passive immunization with antibodies against iron-regulated outer membrane proteins protects turkeys from Escherichia coli septicaemia. Infect Immun 1987; 55:1239–1242
    [Google Scholar]
  9. Sciortino C. V., Finkelstein R. A. Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo . Infect Immun 1983; 42:990–996
    [Google Scholar]
  10. Nassif X., Mazert M.-C., Mounier J., Sansonetti P. J. Evaluation with an iuc.: Tn 10 mutant of the role of aerobactin production in the virulence of Shigella flexneri . Infect Immun 1987; 55:1963–1969
    [Google Scholar]
  11. Levine M. M., Nataro J. P., Karch H. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis 1985; 152:550–559
    [Google Scholar]
  12. Stevenson P., Griffiths E. Growth of Escherichia coli under iron-restricted conditions. In Sussman M. (ed) The virulence of Escherichia coli. (Special publication of the Society for General Microbiology 13.) London: Academic Press; 1985413–417
    [Google Scholar]
  13. Amow L. E. Colorometric determination of the components of 3, 4-dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 1937; 11:531–537
    [Google Scholar]
  14. Carbonetti N. H., Williams P. H. Detection of synthesis of the hydroxamate siderophore aerobactin by pathogenic isolates of Escherichia coli . In Sussman M. (ed) The virulence of Escherichia coli. (Special publication of the Society for General Microbiology 13.) London: Academic Press; 1985419–424
    [Google Scholar]
  15. Achtman M., Mercer A., Kusecek B. Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun 1983; 39:315–335
    [Google Scholar]
  16. Stevenson P., Griffiths E. Detection of antibodies and antigens by immunoblotting. In Sussman M. (ed) The virulence of Escherichia coli. (Special publication of the Society for General Microbiology 13.) London: Academic Press; 1985457–463
    [Google Scholar]
  17. Der Vartanian M. Differences in excretion and efficiency of the aerobactin and enterochelin siderophores in a bovine pathogenic strain of Escherichia coli . Infect Immun 1988; 56:413–418
    [Google Scholar]
  18. Williams P. H., Carbonetti N. H. Iron, siderophores, and the pursuit of virulence: independence of the aerobactin and enterochelin iron uptake systems in Escherichia coli . Infect Immun 1986; 51:942–947
    [Google Scholar]
  19. Stoebner J. A., Payne S. M. Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae . Infect Immun 1988; 56:2891–2895
    [Google Scholar]
  20. Lawlor K. M., Daskaleros P. A., Robinson R. E., Payne S. M. Virulence of iron transport mutants of Shigella flexneri and utilization of host iron compounds. Infect Immun 1987; 55:594–599
    [Google Scholar]
  21. Buetin L., Montenegro M., Zimmermann S., Stephan R. Charac terization of hemolytic strains of Escherichia coli belonging to classical enteropathogenic O-serogroups. Zentralbl Bakteriol Mikrobiol Hyg A 1986; 261:266–279
    [Google Scholar]
  22. Ulshen M. H., Rollo J. L. Pathogenesis of Escherichia coli gas troenteritis in man–another mechanism. N Engl J Med 1980; 302:99–101
    [Google Scholar]
  23. Otto B. R., Sparrius M., Verweij-van Vught A. M. J. J., MacLaren D. M. Iron-regulated outer membrane protein of Bacteroides fragilis involved in heme uptake. Infect Immun 1990; 58:3954–3958
    [Google Scholar]
  24. Chart H., Stevenson P., Griffiths E. Iron-regulated outer-membrane proteins of Escherichia coli strains associated with enteric or extraintestinal diseases of man and animals. J Gen Microbiol 1988; 134:1549–1559
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-37-1-15
Loading
/content/journal/jmm/10.1099/00222615-37-1-15
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error