Adhesion of bacteria to guinea-pig colonic epithelial cells in vitro was inhibited by fucose with all the four strains tested (two of Shigella dysenteriae type 1 and two of S. flexneri). N-acetyl neuraminic acid and N-acetyl mannosamine also caused inhibition, suggesting a multiplicity of receptors on the epithelial cell. Congo-red binding of the strains correlated with their adhesive ability, whereas haemagglutination of rabbit erythrocytes by the bacteria did not.
EvansD. J.,
EvansD. G.,
DuPontH. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea-pig erythrocytes in the presence and absence of mannose. Infect Immun1979; 23:336–346
JonesG. W.,
AbramsG. D.,
FreterR. Adhesive properties of Vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity. Infect Immun1976; 14:232–239
QadriF.,
HossainS. A.,
ČiznarI. Congo red binding and salt aggregation as indicators of virulence in Shigella species. J Clin Microbiol1988; 26:1343–1348
DeanE. A.,
IsaacsonR. E. In vitro adhesion of piliated Escherichia coli to small intestinal villous epithelial cells from rabbits and the identification of a soluble 987P pilus receptor-containing fraction. Infect Immun1982; 36:1192–1198
IzharM.,
NuchamowitzY.,
MirelmanD. Adherence of Shigella flexneri to guinea pig intestinal cells is mediated by a mucosal adhesin. Infect Immun1982; 35:1110–1118